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Article: Tetratricopeptide repeat domain 9A modulates anxiety-like behavior in female mice

TitleTetratricopeptide repeat domain 9A modulates anxiety-like behavior in female mice
Authors
Issue Date2016
PublisherNature Publishing Group: Open Access Journals. The Journal's web site is located at http://www.nature.com/srep/index.html
Citation
Scientific Reports, 2016, v. 6, p. 37568:1-14 How to Cite?
AbstractTetratricopeptide repeat domain 9A (TTC9A) expression is abundantly expressed in the brain. Previous studies in TTC9A knockout (TTC9A−/−) mice have indicated that TTC9A negatively regulates the action of estrogen. In this study we investigated the role of TTC9A on anxiety-like behavior through its functional interaction with estrogen using the TTC9A−/− mice model. A battery of tests on anxiety related behaviors was conducted. Our results demonstrated that TTC9A−/− mice exhibited an increase in anxiety-like behaviors compared to the wild type TTC9A+/+ mice. This difference was abolished after ovariectomy, and administration of 17-β-estradiol benzoate (EB) restored this escalated anxiety-like behavior in TTC9A−/− mice. Since serotonin is well-known to be the key neuromodulator involved in anxiety behaviors, the mRNA levels of tryptophan hydroxylase (TPH) 1, TPH2 (both are involved in serotonin synthesis), and serotonin transporter (5-HTT) were measured in the ventromedial prefrontal cortex (vmPFC) and dorsal raphe nucleus (DRN). Interestingly, the heightened anxiety in TTC9A−/− mice under EB influence is consistent with a greater induction of TPH 2, and 5-HTT by EB in DRN that play key roles in emotion regulation. In conclusion, our data indicate that TTC9A modulates the anxiety-related behaviors through modulation of estrogen action on the serotonergic system in the DRN.
Persistent Identifierhttp://hdl.handle.net/10722/236983
ISSN
2017 Impact Factor: 4.122
2015 SCImago Journal Rankings: 2.073

 

DC FieldValueLanguage
dc.contributor.authorLim, LW-
dc.contributor.authorShrestha, S-
dc.contributor.authorOr, YZ-
dc.contributor.authorTAN, ZKS-
dc.contributor.authorChung, HH-
dc.contributor.authorSun, YANG-
dc.contributor.authorLim, CHEW LENG-
dc.contributor.authorMUHAMMAD SHARAFUDDIN BIN, MK-
dc.contributor.authorLufkin, T-
dc.contributor.authorLin, VCL-
dc.date.accessioned2016-12-20T06:14:24Z-
dc.date.available2016-12-20T06:14:24Z-
dc.date.issued2016-
dc.identifier.citationScientific Reports, 2016, v. 6, p. 37568:1-14-
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/10722/236983-
dc.description.abstractTetratricopeptide repeat domain 9A (TTC9A) expression is abundantly expressed in the brain. Previous studies in TTC9A knockout (TTC9A−/−) mice have indicated that TTC9A negatively regulates the action of estrogen. In this study we investigated the role of TTC9A on anxiety-like behavior through its functional interaction with estrogen using the TTC9A−/− mice model. A battery of tests on anxiety related behaviors was conducted. Our results demonstrated that TTC9A−/− mice exhibited an increase in anxiety-like behaviors compared to the wild type TTC9A+/+ mice. This difference was abolished after ovariectomy, and administration of 17-β-estradiol benzoate (EB) restored this escalated anxiety-like behavior in TTC9A−/− mice. Since serotonin is well-known to be the key neuromodulator involved in anxiety behaviors, the mRNA levels of tryptophan hydroxylase (TPH) 1, TPH2 (both are involved in serotonin synthesis), and serotonin transporter (5-HTT) were measured in the ventromedial prefrontal cortex (vmPFC) and dorsal raphe nucleus (DRN). Interestingly, the heightened anxiety in TTC9A−/− mice under EB influence is consistent with a greater induction of TPH 2, and 5-HTT by EB in DRN that play key roles in emotion regulation. In conclusion, our data indicate that TTC9A modulates the anxiety-related behaviors through modulation of estrogen action on the serotonergic system in the DRN.-
dc.languageeng-
dc.publisherNature Publishing Group: Open Access Journals. The Journal's web site is located at http://www.nature.com/srep/index.html-
dc.relation.ispartofScientific Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleTetratricopeptide repeat domain 9A modulates anxiety-like behavior in female mice-
dc.typeArticle-
dc.identifier.emailLim, LW: limlw@hku.hk-
dc.identifier.authorityLim, LW=rp02088-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/srep37568-
dc.identifier.hkuros270692-
dc.identifier.volume6-
dc.identifier.spage37568:1-
dc.identifier.epage14-
dc.publisher.placeUnited Kingdom-

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