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Article: A comparison of hemagglutination inhibition and neutralization assays for characterizing immunity to seasonal influenza A

TitleA comparison of hemagglutination inhibition and neutralization assays for characterizing immunity to seasonal influenza A
Authors
Issue Date2016
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/subs.asp?ref=1750-2640&site=1
Citation
Influenza and Other Respiratory Viruses, 2016, v. 10 n. 6, p. 518-524 How to Cite?
AbstractBACKGROUND: Serum antibody to influenza can be used to identify past exposure and measure current immune status. The two most common methods for measuring this are the hemagglutination inhibition assay (HI) and the viral neutralization assay (NT), which have not been systematically compared for a large number of influenza viruses. METHODS: A total of 151 study participants from near Guangzhou, China, were enrolled in 2009 and provided serum. HI and NT assays were performed for 12 historic and recently circulating strains of seasonal influenza A. We compared titers using Spearman correlation and fit models to predict NT using HI results. RESULTS: We observed high positive mean correlation between HI and NT assays (Spearman's rank correlation, ρ=.86) across all strains. Correlation was highest within subtypes and within close proximity in time. Overall, an HI=20 corresponded to NT=10, and HI=40 corresponded to NT=20. Linear regression of log(NT) on log(HI) was statistically significant, with age modifying this relationship. Strain-specific area under a curve (AUC) indicated good accuracy (>80%) for predicting NT with HI. CONCLUSIONS: While we found high overall correspondence of titers between NT and HI assays for seasonal influenza A, no exact equivalence between assays could be determined. This was further complicated by correspondence between titers changing with age. These findings support generalized comparison of results between assays and give further support for use of the hemagglutination inhibition assay over the more resource intensive viral neutralization assay for seasonal influenza A, although attention should be given to the effect of age on these assays.
Persistent Identifierhttp://hdl.handle.net/10722/236526
ISSN
2017 Impact Factor: 2.954
2015 SCImago Journal Rankings: 1.570

 

DC FieldValueLanguage
dc.contributor.authorTruelove, S-
dc.contributor.authorZhu, H-
dc.contributor.authorLessler, J-
dc.contributor.authorRiley, S-
dc.contributor.authorRead, JM-
dc.contributor.authorWang, S-
dc.contributor.authorKwok, KO-
dc.contributor.authorGuan, Y-
dc.contributor.authorJiang, CQ-
dc.contributor.authorCummings, DA-
dc.date.accessioned2016-11-25T00:54:37Z-
dc.date.available2016-11-25T00:54:37Z-
dc.date.issued2016-
dc.identifier.citationInfluenza and Other Respiratory Viruses, 2016, v. 10 n. 6, p. 518-524-
dc.identifier.issn1750-2640-
dc.identifier.urihttp://hdl.handle.net/10722/236526-
dc.description.abstractBACKGROUND: Serum antibody to influenza can be used to identify past exposure and measure current immune status. The two most common methods for measuring this are the hemagglutination inhibition assay (HI) and the viral neutralization assay (NT), which have not been systematically compared for a large number of influenza viruses. METHODS: A total of 151 study participants from near Guangzhou, China, were enrolled in 2009 and provided serum. HI and NT assays were performed for 12 historic and recently circulating strains of seasonal influenza A. We compared titers using Spearman correlation and fit models to predict NT using HI results. RESULTS: We observed high positive mean correlation between HI and NT assays (Spearman's rank correlation, ρ=.86) across all strains. Correlation was highest within subtypes and within close proximity in time. Overall, an HI=20 corresponded to NT=10, and HI=40 corresponded to NT=20. Linear regression of log(NT) on log(HI) was statistically significant, with age modifying this relationship. Strain-specific area under a curve (AUC) indicated good accuracy (>80%) for predicting NT with HI. CONCLUSIONS: While we found high overall correspondence of titers between NT and HI assays for seasonal influenza A, no exact equivalence between assays could be determined. This was further complicated by correspondence between titers changing with age. These findings support generalized comparison of results between assays and give further support for use of the hemagglutination inhibition assay over the more resource intensive viral neutralization assay for seasonal influenza A, although attention should be given to the effect of age on these assays.-
dc.languageeng-
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/subs.asp?ref=1750-2640&site=1-
dc.relation.ispartofInfluenza and Other Respiratory Viruses-
dc.rightsThe definitive version is available at www.blackwell-synergy.com-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleA comparison of hemagglutination inhibition and neutralization assays for characterizing immunity to seasonal influenza A-
dc.typeArticle-
dc.identifier.emailZhu, H: zhuhch@hku.hk-
dc.identifier.emailKwok, KO: kkocuhk@hkucc.hku.hk-
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hk-
dc.identifier.authorityZhu, H=rp01535-
dc.identifier.authorityGuan, Y=rp00397-
dc.description.naturepostprint-
dc.identifier.doi10.1111/irv.12408-
dc.identifier.hkuros270634-
dc.identifier.volume10-
dc.identifier.issue6-
dc.identifier.spage518-
dc.identifier.epage524-
dc.publisher.placeUnited Kingdom-

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