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Article: Evaluation of chemically modified SLA implants (modSLA) biofunctionalized with integrin (RGD)- and heparin (KRSR)-binding peptides

TitleEvaluation of chemically modified SLA implants (modSLA) biofunctionalized with integrin (RGD)- and heparin (KRSR)-binding peptides
Authors
Keywordsremoval torque value
extracellular matrix protein binding sequences
histomorphometry
titanium surfaces biofunctionalized with biologically active peptides
chemically modified sandblasted and acid-etched titanium implant surfaces
Issue Date2012
Citation
Journal of Biomedical Materials Research - Part A, 2012, v. 100 A, n. 3, p. 703-711 How to Cite?
AbstractEnhancing osseointegration through surface immobilization of multiple short peptide sequences that mimic extracellular matrix (ECM) proteins, such as arginine-glycine-aspartic acid (RGD) and lysine-arginine-serine-arginine (KRSR), has not yet been extensively explored. Additionally, the effect of biofunctionalizing chemically modified sandblasted and acid-etched surfaces (modSLA) is unknown. The present study evaluated modSLA implant surfaces modified with RGD and KRSR for potentially enhanced effects on bone apposition and interfacial shear strength during early stages of bone regeneration. Two sets of experimental implants were placed in the maxillae of eight miniature pigs, known for their rapid wound healing kinetics: bone chamber implants creating two circular bone defects for histomorphometric analysis on one side and standard thread configuration implants for removal torque testing on the other side. Three different biofunctionalized modSLA surfaces using poly-L-lysine-graft-poly(ethylene glycol) (PLL-g-PEG) as a carrier minimizing nonspecific protein adsorption [(i) 20 pmol cm -2 KRSR alone (KRSR); or in combination with RGD in two different concentrations; (ii) 0.05 pmol cm -2 RGD (KRSR/RGD-1); (iii) 1.26 pmol cm -2 RGD (KRSR/RGD-2)] were compared with (iv) control modSLA. Animals were sacrificed at 2 weeks. Removal torque values (701.48-780.28 N mm), bone-to-implant contact (BIC) (35.22%-41.49%), and new bone fill (28.58%-30.62%) demonstrated no significant differences among treatments. It may be concluded that biofunctionalizing modSLA surfaces with KRSR and RGD derivatives of PLL-g-PEG polymer does not increase BIC, bone fill, or interfacial shear strength. Copyright © 2011 Wiley Periodicals, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/236191
ISSN
2017 Impact Factor: 3.231
2015 SCImago Journal Rankings: 0.979
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBroggini, Nina-
dc.contributor.authorTosatti, Samuele-
dc.contributor.authorFerguson, Stephen J.-
dc.contributor.authorSchuler, Martin-
dc.contributor.authorTextor, Marcus-
dc.contributor.authorBornstein, Michael M.-
dc.contributor.authorBosshardt, Dieter D.-
dc.contributor.authorBuser, Daniel-
dc.date.accessioned2016-11-11T07:43:11Z-
dc.date.available2016-11-11T07:43:11Z-
dc.date.issued2012-
dc.identifier.citationJournal of Biomedical Materials Research - Part A, 2012, v. 100 A, n. 3, p. 703-711-
dc.identifier.issn1549-3296-
dc.identifier.urihttp://hdl.handle.net/10722/236191-
dc.description.abstractEnhancing osseointegration through surface immobilization of multiple short peptide sequences that mimic extracellular matrix (ECM) proteins, such as arginine-glycine-aspartic acid (RGD) and lysine-arginine-serine-arginine (KRSR), has not yet been extensively explored. Additionally, the effect of biofunctionalizing chemically modified sandblasted and acid-etched surfaces (modSLA) is unknown. The present study evaluated modSLA implant surfaces modified with RGD and KRSR for potentially enhanced effects on bone apposition and interfacial shear strength during early stages of bone regeneration. Two sets of experimental implants were placed in the maxillae of eight miniature pigs, known for their rapid wound healing kinetics: bone chamber implants creating two circular bone defects for histomorphometric analysis on one side and standard thread configuration implants for removal torque testing on the other side. Three different biofunctionalized modSLA surfaces using poly-L-lysine-graft-poly(ethylene glycol) (PLL-g-PEG) as a carrier minimizing nonspecific protein adsorption [(i) 20 pmol cm -2 KRSR alone (KRSR); or in combination with RGD in two different concentrations; (ii) 0.05 pmol cm -2 RGD (KRSR/RGD-1); (iii) 1.26 pmol cm -2 RGD (KRSR/RGD-2)] were compared with (iv) control modSLA. Animals were sacrificed at 2 weeks. Removal torque values (701.48-780.28 N mm), bone-to-implant contact (BIC) (35.22%-41.49%), and new bone fill (28.58%-30.62%) demonstrated no significant differences among treatments. It may be concluded that biofunctionalizing modSLA surfaces with KRSR and RGD derivatives of PLL-g-PEG polymer does not increase BIC, bone fill, or interfacial shear strength. Copyright © 2011 Wiley Periodicals, Inc.-
dc.languageeng-
dc.relation.ispartofJournal of Biomedical Materials Research - Part A-
dc.subjectremoval torque value-
dc.subjectextracellular matrix protein binding sequences-
dc.subjecthistomorphometry-
dc.subjecttitanium surfaces biofunctionalized with biologically active peptides-
dc.subjectchemically modified sandblasted and acid-etched titanium implant surfaces-
dc.titleEvaluation of chemically modified SLA implants (modSLA) biofunctionalized with integrin (RGD)- and heparin (KRSR)-binding peptides-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jbm.a.34004-
dc.identifier.pmid22213622-
dc.identifier.scopuseid_2-s2.0-84856235129-
dc.identifier.volume100 A-
dc.identifier.issue3-
dc.identifier.spage703-
dc.identifier.epage711-
dc.identifier.eissn1552-4965-
dc.identifier.isiWOS:000299414400018-

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