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Article: Effects of methotrexate on the viscoelastic properties of single cells probed by atomic force microscopy

TitleEffects of methotrexate on the viscoelastic properties of single cells probed by atomic force microscopy
Authors
Issue Date2016
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0092-0606
Citation
Journal of Biological Physics, 2016, v. 42 n. 4, p. 551-569 How to Cite?
AbstractMethotrexate is a commonly used anti-cancer chemotherapy drug. Cellular mechanical properties are fundamental parameters that reflect the physiological state of a cell. However, so far the role of cellular mechanical properties in the actions of methotrexate is still unclear. In recent years, probing the behaviors of single cells with the use of atomic force microscopy (AFM) has contributed much to the field of cell biomechanics. In this work, with the use of AFM, the effects of methotrexate on the viscoelastic properties of four types of cells were quantitatively investigated. The inhibitory and cytotoxic effects of methotrexate on the proliferation of cells were observed by optical and fluorescence microscopy. AFM indenting was used to measure the changes of cellular viscoelastic properties (Young’s modulus and relaxation time) by using both conical tip and spherical tip, quantitatively showing that the stimulation of methotrexate resulted in a significant decrease of both cellular Young’s modulus and relaxation times. The morphological changes of cells induced by methotrexate were visualized by AFM imaging. The study improves our understanding of methotrexate action and offers a novel way to quantify drug actions at the single-cell level by measuring cellular viscoelastic properties, which may have potential impacts on developing label-free methods for drug evaluation.
Persistent Identifierhttp://hdl.handle.net/10722/234597
ISSN
2015 Impact Factor: 1.394
2015 SCImago Journal Rankings: 0.481

 

DC FieldValueLanguage
dc.contributor.authorLI, M-
dc.contributor.authorLIU, L-
dc.contributor.authorXIAO, X-
dc.contributor.authorXi, N-
dc.contributor.authorWANG, Y-
dc.date.accessioned2016-10-14T13:47:56Z-
dc.date.available2016-10-14T13:47:56Z-
dc.date.issued2016-
dc.identifier.citationJournal of Biological Physics, 2016, v. 42 n. 4, p. 551-569-
dc.identifier.issn0092-0606-
dc.identifier.urihttp://hdl.handle.net/10722/234597-
dc.description.abstractMethotrexate is a commonly used anti-cancer chemotherapy drug. Cellular mechanical properties are fundamental parameters that reflect the physiological state of a cell. However, so far the role of cellular mechanical properties in the actions of methotrexate is still unclear. In recent years, probing the behaviors of single cells with the use of atomic force microscopy (AFM) has contributed much to the field of cell biomechanics. In this work, with the use of AFM, the effects of methotrexate on the viscoelastic properties of four types of cells were quantitatively investigated. The inhibitory and cytotoxic effects of methotrexate on the proliferation of cells were observed by optical and fluorescence microscopy. AFM indenting was used to measure the changes of cellular viscoelastic properties (Young’s modulus and relaxation time) by using both conical tip and spherical tip, quantitatively showing that the stimulation of methotrexate resulted in a significant decrease of both cellular Young’s modulus and relaxation times. The morphological changes of cells induced by methotrexate were visualized by AFM imaging. The study improves our understanding of methotrexate action and offers a novel way to quantify drug actions at the single-cell level by measuring cellular viscoelastic properties, which may have potential impacts on developing label-free methods for drug evaluation.-
dc.languageeng-
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0092-0606-
dc.relation.ispartofJournal of Biological Physics-
dc.rightsThe final publication is available at Springer via http://dx.doi.org/[insert DOI]-
dc.titleEffects of methotrexate on the viscoelastic properties of single cells probed by atomic force microscopy-
dc.typeArticle-
dc.identifier.emailXi, N: xining@hku.hk-
dc.identifier.authorityXi, N=rp02044-
dc.identifier.doi10.1007/s10867-016-9423-6-
dc.identifier.hkuros269167-
dc.identifier.volume42-
dc.identifier.issue4-
dc.identifier.spage551-
dc.identifier.epage569-
dc.publisher.placeNetherlands-

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