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Article: Single Cell Transfection through Precise Microinjection with Quantitatively Controlled Injection Volumes

TitleSingle Cell Transfection through Precise Microinjection with Quantitatively Controlled Injection Volumes
Authors
Issue Date2016
PublisherNature Publishing Group: Open Access Journals. The Journal's web site is located at http://www.nature.com/srep/index.html
Citation
Scientific Reports, 2016, v. 6, p. 24127:1-9 How to Cite?
AbstractCell transfection is a technique wherein foreign genetic molecules are delivered into cells. To elucidate distinct responses during cell genetic modification, methods to achieve transfection at the single-cell level are of great value. Herein, we developed an automated micropipette-based quantitative microinjection technology that can deliver precise amounts of materials into cells. The developed microinjection system achieved precise single-cell microinjection by pre-patterning cells in an array and controlling the amount of substance delivered based on injection pressure and time. The precision of the proposed injection technique was examined by comparing the fluorescence intensities of fluorescent dye droplets with a standard concentration and water droplets with a known injection amount of the dye in oil. Injection of synthetic modified mRNA (modRNA) encoding green fluorescence proteins or a cocktail of plasmids encoding green and red fluorescence proteins into human foreskin fibroblast cells demonstrated that the resulting green fluorescence intensity or green/red fluorescence intensity ratio were well correlated with the amount of genetic material injected into the cells. Single-cell transfection via the developed microinjection technique will be of particular use in cases where cell transfection is challenging and genetically modified of selected cells are desired
Persistent Identifierhttp://hdl.handle.net/10722/234430
ISSN
2015 Impact Factor: 5.228
2015 SCImago Journal Rankings: 2.073

 

DC FieldValueLanguage
dc.contributor.authorChow, YT-
dc.contributor.authorChen, S-
dc.contributor.authorWang, R-
dc.contributor.authorLiu, C-
dc.contributor.authorKong, MCW-
dc.contributor.authorLi, RA-
dc.contributor.authorCheng, SH-
dc.contributor.authorSun, D-
dc.date.accessioned2016-10-14T13:46:50Z-
dc.date.available2016-10-14T13:46:50Z-
dc.date.issued2016-
dc.identifier.citationScientific Reports, 2016, v. 6, p. 24127:1-9-
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/10722/234430-
dc.description.abstractCell transfection is a technique wherein foreign genetic molecules are delivered into cells. To elucidate distinct responses during cell genetic modification, methods to achieve transfection at the single-cell level are of great value. Herein, we developed an automated micropipette-based quantitative microinjection technology that can deliver precise amounts of materials into cells. The developed microinjection system achieved precise single-cell microinjection by pre-patterning cells in an array and controlling the amount of substance delivered based on injection pressure and time. The precision of the proposed injection technique was examined by comparing the fluorescence intensities of fluorescent dye droplets with a standard concentration and water droplets with a known injection amount of the dye in oil. Injection of synthetic modified mRNA (modRNA) encoding green fluorescence proteins or a cocktail of plasmids encoding green and red fluorescence proteins into human foreskin fibroblast cells demonstrated that the resulting green fluorescence intensity or green/red fluorescence intensity ratio were well correlated with the amount of genetic material injected into the cells. Single-cell transfection via the developed microinjection technique will be of particular use in cases where cell transfection is challenging and genetically modified of selected cells are desired-
dc.languageeng-
dc.publisherNature Publishing Group: Open Access Journals. The Journal's web site is located at http://www.nature.com/srep/index.html-
dc.relation.ispartofScientific Reports-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleSingle Cell Transfection through Precise Microinjection with Quantitatively Controlled Injection Volumes-
dc.typeArticle-
dc.identifier.emailKong, MCW: mkcwkong@hku.hk-
dc.identifier.emailLi, RA: ronaldli@hkucc.hku.hk-
dc.identifier.authorityKong, MCW=rp01563-
dc.identifier.authorityLi, RA=rp01352-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/srep24127-
dc.identifier.hkuros269099-
dc.identifier.volume6-
dc.identifier.spage24127:1-
dc.identifier.epage9-
dc.publisher.placeUnited Kingdom-

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