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Article: Gordonia hongkongensis sp. nov., isolated from blood culture and peritoneal dialysis effluent of patients in Hong Kong

TitleGordonia hongkongensis sp. nov., isolated from blood culture and peritoneal dialysis effluent of patients in Hong Kong
Authors
KeywordsBlood
Gordonia
Hongkongensis
Novel
Peritoneal dialysis effluent
Issue Date2016
PublisherSociety for General Microbiology. The Journal's web site is located at http://ijs.sgmjournals.org
Citation
International Journal of Systematic and Evolutionary Microbiology, 2016, v. 66, p. 3942-3950 How to Cite?
AbstractTwo bacterial strains, HKU50Tand HKU46, were isolated in Hong Kong from the blood culture and the peritoneal dialysis effluent of two patients. The strains are Gram-stain-positive, acid-fast, non-motile, nonsporulating bacilli. They grow on Columbia agar with 5%defibrinated sheep blood and brain–heart infusion agar under aerobic conditions with 5%CO2at 37°C as pink-to-orange, non-haemolytic colonies. The strains are catalase-positive and oxidase-negative, and have a unique biochemical profile distinguishable from other closely related species. DNA sequencing revealed that both isolates possessed multiple intra-genomic 16S rRNA gene copies (99.8–100%sequence identities to Gordonia lacunae NRRL B-24551Tand Gordonia terrae NRRL B-16283T). Phylogenetic analysis of the 16S rRNA gene, secA1 and gyrB showed that the two isolates formed a distinct branch within the genus Gordonia and were most closely related to G. lacunae and G. terrae. DNA–DNA hybridization demonstrated ≤ 53.7% and ≤ 49.4%DNA relatedness between the two isolates and G. lacunae, and between the two isolates and G. terrae, respectively. Hierarchical cluster analysis of MALDI–TOF MS main spectrum profiles showed that strains HKU50T and HKU46 were closely related to each other, but were distinct from G. lacunae, G. terrae, or any other species of the genus Gordonia in the Bruker database. The chemotaxonomic traits of the two strains were highly similar, and the major fatty acids were summed feature 4 (iso-C15: 02-OH/C16: 1trans-9), C16: 0, C18: 1cis-9, and tuberculostearic acid. A novel species named Gordonia hongkongensis sp. nov. is proposed to accommodate strains HKU50Tand HKU46, with strain HKU50T (=CCOS 955T=CIP 111027T=NBRC 111234T=NCCP 16210T) as the type strain.
Persistent Identifierhttp://hdl.handle.net/10722/234070
ISSN
2017 Impact Factor: 1.932
2015 SCImago Journal Rankings: 1.088
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTsang, CC-
dc.contributor.authorXiong, L-
dc.contributor.authorPoon, WSR-
dc.contributor.authorChen, HKJ-
dc.contributor.authorLeung, KW-
dc.contributor.authorLam, JYW-
dc.contributor.authorWu, AKL-
dc.contributor.authorChan, JFW-
dc.contributor.authorLau, SKP-
dc.contributor.authorWoo, PCY-
dc.date.accessioned2016-10-14T06:58:52Z-
dc.date.available2016-10-14T06:58:52Z-
dc.date.issued2016-
dc.identifier.citationInternational Journal of Systematic and Evolutionary Microbiology, 2016, v. 66, p. 3942-3950-
dc.identifier.issn1466-5026-
dc.identifier.urihttp://hdl.handle.net/10722/234070-
dc.description.abstractTwo bacterial strains, HKU50Tand HKU46, were isolated in Hong Kong from the blood culture and the peritoneal dialysis effluent of two patients. The strains are Gram-stain-positive, acid-fast, non-motile, nonsporulating bacilli. They grow on Columbia agar with 5%defibrinated sheep blood and brain–heart infusion agar under aerobic conditions with 5%CO2at 37°C as pink-to-orange, non-haemolytic colonies. The strains are catalase-positive and oxidase-negative, and have a unique biochemical profile distinguishable from other closely related species. DNA sequencing revealed that both isolates possessed multiple intra-genomic 16S rRNA gene copies (99.8–100%sequence identities to Gordonia lacunae NRRL B-24551Tand Gordonia terrae NRRL B-16283T). Phylogenetic analysis of the 16S rRNA gene, secA1 and gyrB showed that the two isolates formed a distinct branch within the genus Gordonia and were most closely related to G. lacunae and G. terrae. DNA–DNA hybridization demonstrated ≤ 53.7% and ≤ 49.4%DNA relatedness between the two isolates and G. lacunae, and between the two isolates and G. terrae, respectively. Hierarchical cluster analysis of MALDI–TOF MS main spectrum profiles showed that strains HKU50T and HKU46 were closely related to each other, but were distinct from G. lacunae, G. terrae, or any other species of the genus Gordonia in the Bruker database. The chemotaxonomic traits of the two strains were highly similar, and the major fatty acids were summed feature 4 (iso-C15: 02-OH/C16: 1trans-9), C16: 0, C18: 1cis-9, and tuberculostearic acid. A novel species named Gordonia hongkongensis sp. nov. is proposed to accommodate strains HKU50Tand HKU46, with strain HKU50T (=CCOS 955T=CIP 111027T=NBRC 111234T=NCCP 16210T) as the type strain.-
dc.languageeng-
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://ijs.sgmjournals.org-
dc.relation.ispartofInternational Journal of Systematic and Evolutionary Microbiology-
dc.rightsInternational Journal of Systematic and Evolutionary Microbiology. Copyright © Society for General Microbiology.-
dc.subjectBlood-
dc.subjectGordonia-
dc.subjectHongkongensis-
dc.subjectNovel-
dc.subjectPeritoneal dialysis effluent-
dc.titleGordonia hongkongensis sp. nov., isolated from blood culture and peritoneal dialysis effluent of patients in Hong Kong-
dc.typeArticle-
dc.identifier.emailXiong, L: lfxiong@hku.hk-
dc.identifier.emailPoon, WSR: rosana@hkucc.hku.hk-
dc.identifier.emailChen, HKJ: jonchk@hku.hk-
dc.identifier.emailWu, AKL: alanklwu@hkucc.hku.hk-
dc.identifier.emailChan, JFW: jfwchan@hku.hk-
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hk-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.authorityTsang, CC=rp02492-
dc.identifier.authorityChan, JFW=rp01736-
dc.identifier.authorityLau, SKP=rp00486-
dc.identifier.authorityWoo, PCY=rp00430-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1099/ijsem.0.001292-
dc.identifier.pmid27406710-
dc.identifier.scopuseid_2-s2.0-84994481889-
dc.identifier.hkuros267611-
dc.identifier.volume66-
dc.identifier.spage3942-
dc.identifier.epage3950-
dc.identifier.isiWOS:000386871600029-
dc.publisher.placeUnited Kingdom-

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