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Conference Paper: Quantification of influenza virus RNA in aerosols in pediatrics patient rooms

TitleQuantification of influenza virus RNA in aerosols in pediatrics patient rooms
Authors
Issue Date2016
PublisherInternational Society for Influenza and Other Respiratory Virus Diseases.
Citation
The 9th International Scientific Conference of Options for the Control of Influenza (Options-9), Chicago, IL., 24-28 August 2016. In Conference Program, 2016, p. 118, abstract no. P-101 How to Cite?
AbstractBACKGROUND: Children are considered as the key driver of influenza transmission, and the potential for human influenza viruses to spread through fine particle aerosols from children remains controversial. The objective of our study was to determine whether influenza viruses could be detected in fine particles in hospital pediatrics patient rooms. METHOD: We sampled the air in 5-bed pediatrics patient rooms for four hours, placing two cyclone air samplers at different distances from the bed with pediatrics patient with laboratory-confirmed influenza infection. We collected air samples in 3 size fractions, and tested the samples by reverse transcription polymerase chain reaction (RT-PCR). RESULTS: We collected air samples in 10 occasions with at least one pediatrics patient with laboratory-confirmed influenza infection in the patient room. We recovered influenza A virus RNA from 7/10 collections (70%): influenza A virus RNA can be recovered in particles>4µm in 7/10, in particles 1-4µm in 3/10, and in particles<1µm in 4/10 collections. CONCLUSION: Frequent detection of influenza virus RNA in aerosols in the proximity of pediatric patients suggests that children could have an important contribution to influenza transmission via the aerosol route.
DescriptionPoster Sessions: no. P-101
Persistent Identifierhttp://hdl.handle.net/10722/233612

 

DC FieldValueLanguage
dc.contributor.authorShiu, EYC-
dc.contributor.authorLeung, NHL-
dc.contributor.authorHuang, WW-
dc.contributor.authorXie, Y-
dc.contributor.authorChu, KW-
dc.contributor.authorSeto, WH-
dc.contributor.authorIp, DKM-
dc.contributor.authorYen, H-
dc.contributor.authorLi, Y-
dc.contributor.authorMok, KP-
dc.contributor.authorPeiris, JSM-
dc.contributor.authorYang, Z-
dc.contributor.authorCowling, BJ-
dc.contributor.authorYe, D-
dc.date.accessioned2016-09-20T05:37:57Z-
dc.date.available2016-09-20T05:37:57Z-
dc.date.issued2016-
dc.identifier.citationThe 9th International Scientific Conference of Options for the Control of Influenza (Options-9), Chicago, IL., 24-28 August 2016. In Conference Program, 2016, p. 118, abstract no. P-101-
dc.identifier.urihttp://hdl.handle.net/10722/233612-
dc.descriptionPoster Sessions: no. P-101-
dc.description.abstractBACKGROUND: Children are considered as the key driver of influenza transmission, and the potential for human influenza viruses to spread through fine particle aerosols from children remains controversial. The objective of our study was to determine whether influenza viruses could be detected in fine particles in hospital pediatrics patient rooms. METHOD: We sampled the air in 5-bed pediatrics patient rooms for four hours, placing two cyclone air samplers at different distances from the bed with pediatrics patient with laboratory-confirmed influenza infection. We collected air samples in 3 size fractions, and tested the samples by reverse transcription polymerase chain reaction (RT-PCR). RESULTS: We collected air samples in 10 occasions with at least one pediatrics patient with laboratory-confirmed influenza infection in the patient room. We recovered influenza A virus RNA from 7/10 collections (70%): influenza A virus RNA can be recovered in particles>4µm in 7/10, in particles 1-4µm in 3/10, and in particles<1µm in 4/10 collections. CONCLUSION: Frequent detection of influenza virus RNA in aerosols in the proximity of pediatric patients suggests that children could have an important contribution to influenza transmission via the aerosol route.-
dc.languageeng-
dc.publisherInternational Society for Influenza and Other Respiratory Virus Diseases.-
dc.relation.ispartofInternational Scientific Conference of Options for the Control of Influenza, Options-9-
dc.titleQuantification of influenza virus RNA in aerosols in pediatrics patient rooms-
dc.typeConference_Paper-
dc.identifier.emailShiu, EYC: eyshiu@HKUCC-COM.hku.hk-
dc.identifier.emailLeung, NHL: nanleung@connect.hku.hk-
dc.identifier.emailXie, Y: yjxie@hku.hk-
dc.identifier.emailChu, KW: dkwchu@hku.hk-
dc.identifier.emailSeto, WH: whseto@hkucc.hku.hk-
dc.identifier.emailIp, DKM: dkmip@hku.hk-
dc.identifier.emailYen, H: hyen@hku.hk-
dc.identifier.emailMok, KP: ch02mkp@hkucc.hku.hk-
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hk-
dc.identifier.emailCowling, BJ: bcowling@hku.hk-
dc.identifier.authorityIp, DKM=rp00256-
dc.identifier.authorityYen, H=rp00304-
dc.identifier.authorityMok, KP=rp01805-
dc.identifier.authorityPeiris, JSM=rp00410-
dc.identifier.authorityCowling, BJ=rp01326-
dc.identifier.hkuros266503-
dc.identifier.hkuros270570-
dc.identifier.spage118, abstract no. P-101-
dc.identifier.epage118, abstract no. P-101-
dc.publisher.placeUnited States-

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