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Conference Paper: Small molecule approach to differentiation of human induced pluripotent stem cells to sensory neurons

TitleSmall molecule approach to differentiation of human induced pluripotent stem cells to sensory neurons
Authors
Issue Date2016
PublisherThe University of Hong Kong.
Citation
The 2016 Neuroscience Symposium and Annual Scientific Conference of the Hong Kong Society of Neurosciences, The University of Hong Kong, Hong Kong, 18 May 2016. In Programme Book, 2016, p. 30, abstract no. P4 How to Cite?
AbstractIn this study, we took an alternative approach and selected five small molecule inhibitors (SMIs) of key signaling pathways to test out a new protocol for the derivation of sensory neurons from human iPSCs. Within 8 days of the differentiation protocol, iPSC-derived sensory neurons were achieved at >80% efficiency. The derived cells were positive for cytoskeletal markers common to neurons, Tuj-1 and neurofilament, and specific markers for sensory neurons, Islet, peripherin and Brn3a. Whole-cell patch-clamp recordings of the neurons showed firing in response to membrane depolarization, capable of generating action potentials sensitive to tetrodotoxin. In co-culture with rat Schwann cells in myelinating medium, axon bundles of the derived sensory neurons underwent myelination, showing internodal segments that were positive for myelin basic protein. The phenotype of the iPSC-derived sensory neurons was sustainable in Neurobasal medium supplemented with maintenance growth factors but without SMIs. Our rapid and efficient induction protocol promises controlled production of sensory neurons from human iPSCs as a pool for developmental studies and disease modeling.
DescriptionConference Theme: Nature and Nurture in Brain Functions
Persistent Identifierhttp://hdl.handle.net/10722/231493

 

DC FieldValueLanguage
dc.contributor.authorCai, S-
dc.contributor.authorHan, L-
dc.contributor.authorChan, YS-
dc.contributor.authorShum, DKY-
dc.date.accessioned2016-09-20T05:23:31Z-
dc.date.available2016-09-20T05:23:31Z-
dc.date.issued2016-
dc.identifier.citationThe 2016 Neuroscience Symposium and Annual Scientific Conference of the Hong Kong Society of Neurosciences, The University of Hong Kong, Hong Kong, 18 May 2016. In Programme Book, 2016, p. 30, abstract no. P4-
dc.identifier.urihttp://hdl.handle.net/10722/231493-
dc.descriptionConference Theme: Nature and Nurture in Brain Functions-
dc.description.abstractIn this study, we took an alternative approach and selected five small molecule inhibitors (SMIs) of key signaling pathways to test out a new protocol for the derivation of sensory neurons from human iPSCs. Within 8 days of the differentiation protocol, iPSC-derived sensory neurons were achieved at >80% efficiency. The derived cells were positive for cytoskeletal markers common to neurons, Tuj-1 and neurofilament, and specific markers for sensory neurons, Islet, peripherin and Brn3a. Whole-cell patch-clamp recordings of the neurons showed firing in response to membrane depolarization, capable of generating action potentials sensitive to tetrodotoxin. In co-culture with rat Schwann cells in myelinating medium, axon bundles of the derived sensory neurons underwent myelination, showing internodal segments that were positive for myelin basic protein. The phenotype of the iPSC-derived sensory neurons was sustainable in Neurobasal medium supplemented with maintenance growth factors but without SMIs. Our rapid and efficient induction protocol promises controlled production of sensory neurons from human iPSCs as a pool for developmental studies and disease modeling.-
dc.languageeng-
dc.publisherThe University of Hong Kong.-
dc.relation.ispartofNeuroscience Symposium & Annual Scientific Conference of the Hong Kong Society of Neurosciences-
dc.titleSmall molecule approach to differentiation of human induced pluripotent stem cells to sensory neurons-
dc.typeConference_Paper-
dc.identifier.emailCai, S: caisa@hku.hk-
dc.identifier.emailHan, L: rahanlei@hku.hk-
dc.identifier.emailChan, YS: yschan@hku.hk-
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hk-
dc.identifier.authorityChan, YS=rp00318-
dc.identifier.authorityShum, DKY=rp00321-
dc.identifier.hkuros266192-
dc.identifier.hkuros267854-
dc.identifier.spage30, abstract no. P4-
dc.identifier.epage30, abstract no. P4-
dc.publisher.placeHong Kong-

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