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Conference Paper: Schwann cells demonstrated lineage plasticity in culture to acquire oligodendrocyte phenotypes

TitleSchwann cells demonstrated lineage plasticity in culture to acquire oligodendrocyte phenotypes
Authors
Issue Date2016
PublisherThe University of Hong Kong.
Citation
The 2016 Neuroscience Symposium and Annual Scientific Conference of the Hong Kong Society of Neurosciences, The University of Hong Kong, Hong Kong, 18 May 2016. In Programme Book, 2016, p. 23, abstract no. OP5 How to Cite?
AbstractSchwann cells and oligodendrocytes are myelin-forming glial cells found in the peripheral and central nervous systems, respectively. Despite being similar in function, the two cell types have distinct developmental origins. In cultured Schwann cells, however, we serendipitously observed expression of the oligodendrocyte lineage fate determining factor Olig2. Purified Schwann cell cultures were prepared from neonatal rat sciatic nerves. Olig2-positive Schwann cells (OL2- SCs) were detected at 25-30 DIV. By 50-60 DIV, OL2-SCs acquired polydendritic morphology typical of oligodendrocyte precursors. This was accompanied by a decline in Schwann cell marker expression. The OP-like cells were termed Schwann cell-derived oligodendrocyte precursors (SC-OP). We therefore hypothesized that the peripheral nervous system environment is essential for the maintenance of Schwann cell identity. Co-culture of OL2-SCs with dorsal root ganglia neurons prevented conversion in SC-OPs. In contrast, SC-OPs co-cultured with dorsal root ganglia neurons continued differentiation into mature oligodendrocyte-like cells with myelin basic protein-positive segments along multiple axons. Our results revealed 'lineage switching' capability of Schwann cells isolated from the peripheral nervous system. Preservation of Schwann cell identity in vitro requires signalling cues derived from peripheral neurons.
DescriptionConference Theme: Nature and Nurture in Brain Functions
Persistent Identifierhttp://hdl.handle.net/10722/231466

 

DC FieldValueLanguage
dc.contributor.authorTsui, YP-
dc.contributor.authorTam, KW-
dc.contributor.authorShum, DKY-
dc.contributor.authorChan, YS-
dc.date.accessioned2016-09-20T05:23:19Z-
dc.date.available2016-09-20T05:23:19Z-
dc.date.issued2016-
dc.identifier.citationThe 2016 Neuroscience Symposium and Annual Scientific Conference of the Hong Kong Society of Neurosciences, The University of Hong Kong, Hong Kong, 18 May 2016. In Programme Book, 2016, p. 23, abstract no. OP5-
dc.identifier.urihttp://hdl.handle.net/10722/231466-
dc.descriptionConference Theme: Nature and Nurture in Brain Functions-
dc.description.abstractSchwann cells and oligodendrocytes are myelin-forming glial cells found in the peripheral and central nervous systems, respectively. Despite being similar in function, the two cell types have distinct developmental origins. In cultured Schwann cells, however, we serendipitously observed expression of the oligodendrocyte lineage fate determining factor Olig2. Purified Schwann cell cultures were prepared from neonatal rat sciatic nerves. Olig2-positive Schwann cells (OL2- SCs) were detected at 25-30 DIV. By 50-60 DIV, OL2-SCs acquired polydendritic morphology typical of oligodendrocyte precursors. This was accompanied by a decline in Schwann cell marker expression. The OP-like cells were termed Schwann cell-derived oligodendrocyte precursors (SC-OP). We therefore hypothesized that the peripheral nervous system environment is essential for the maintenance of Schwann cell identity. Co-culture of OL2-SCs with dorsal root ganglia neurons prevented conversion in SC-OPs. In contrast, SC-OPs co-cultured with dorsal root ganglia neurons continued differentiation into mature oligodendrocyte-like cells with myelin basic protein-positive segments along multiple axons. Our results revealed 'lineage switching' capability of Schwann cells isolated from the peripheral nervous system. Preservation of Schwann cell identity in vitro requires signalling cues derived from peripheral neurons.-
dc.languageeng-
dc.publisherThe University of Hong Kong.-
dc.relation.ispartofNeuroscience Symposium & Annual Scientific Conference of the Hong Kong Society of Neurosciences-
dc.titleSchwann cells demonstrated lineage plasticity in culture to acquire oligodendrocyte phenotypes-
dc.typeConference_Paper-
dc.identifier.emailTsui, YP: alex2013@hku.hk-
dc.identifier.emailTam, KW: tamkw@hku.hk-
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hk-
dc.identifier.emailChan, YS: yschan@hku.hk-
dc.identifier.authorityShum, DKY=rp00321-
dc.identifier.authorityChan, YS=rp00318-
dc.identifier.hkuros263578-
dc.identifier.spage23, abstract no. OP5-
dc.identifier.epage23, abstract no. OP5-
dc.publisher.placeHong Kong-

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