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Article: Interleukin-6 polymorphisms are associated with pathogenic bacteria in subjects with periodontitis

TitleInterleukin-6 polymorphisms are associated with pathogenic bacteria in subjects with periodontitis
Authors
KeywordsInterleukin-6
Bacteria
Genetic polymorphisms
Periodontitis
Issue Date2008
Citation
Journal of Periodontology, 2008, v. 79, n. 4, p. 677-683 How to Cite?
AbstractBackground: Growing evidence suggests that individual genetic susceptibility may influence the host's response to infections. Previously, we showed that a common variation in the interleukin (IL)-6 gene was associated with increased odds of detection of common periodontal pathogens from individuals with aggressive periodontitis. The aim of this study was to investigate the association between IL-6 polymorphisms and periodontopathogenic bacteria in a larger, ethnically mixed population of subjects with periodontitis. Methods: Genomic DNA was extracted from 107 subjects diagnosed with severe forms of periodontitis to study a cluster of polymorphisms in inflammatory genes, including IL-6. The presence of Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans), Porphyromonas gingivalis, and Tannereila forsythia (previously T. forsythensis) in their subgingival biofilm was determined by polymerase chain reaction analysis. Serum IL-6 and C-reactive protein (CRP) concentrations were analyzed by enzyme-linked immunosorbent assay. Results: Multiple logistic regression analysis revealed that the IL-6 -6106 polymorphism was associated with the detection of A. actinomycetemcomitans (P = 0.009; odds ratio [OR] = 3.5; 95% confidence interval [CI]: 1.38 to 9.16) and the concomitant detection of A. actinomycetemcomitans and P. gingivalis (P = 0.015; OR = 3.6; 95% CI: 1.28 to 10.04). The IL-6 -174 polymorphism was associated with increased odds of the concomitant detection of A. actinomycetemcomitans and P. gingivalis (P = 0.042; OR = 2.8; 95% CI: 1.04 to 7.75). Haplotype analysis of all five IL-6 polymorphisms confirmed an association with the detection of A. actinomycetemcomitans (P = 0.046). The IL-6 -6106 polymorphism was also associated with CRP serum levels at multivariate analysis (P = 0.024). Conclusions: These findings confirm the hypothesis that complex interactions between the microbiota and host genome are at the basis of susceptibility to periodontitis. Periodontal disease may represent a useful model to study the pathways and mechanisms of host-pathogen interactions in inflammatory diseases.
Persistent Identifierhttp://hdl.handle.net/10722/230807
ISSN
2015 Impact Factor: 2.844
2015 SCImago Journal Rankings: 1.070

 

DC FieldValueLanguage
dc.contributor.authorNibali, Luigi-
dc.contributor.authorTonetti, Maurizio S.-
dc.contributor.authorReady, Derren-
dc.contributor.authorParkar, Mohamed-
dc.contributor.authorBrett, Peter M.-
dc.contributor.authorDonos, Nikos-
dc.contributor.authorD'Aiuto, Francesco-
dc.date.accessioned2016-09-01T06:06:51Z-
dc.date.available2016-09-01T06:06:51Z-
dc.date.issued2008-
dc.identifier.citationJournal of Periodontology, 2008, v. 79, n. 4, p. 677-683-
dc.identifier.issn0022-3492-
dc.identifier.urihttp://hdl.handle.net/10722/230807-
dc.description.abstractBackground: Growing evidence suggests that individual genetic susceptibility may influence the host's response to infections. Previously, we showed that a common variation in the interleukin (IL)-6 gene was associated with increased odds of detection of common periodontal pathogens from individuals with aggressive periodontitis. The aim of this study was to investigate the association between IL-6 polymorphisms and periodontopathogenic bacteria in a larger, ethnically mixed population of subjects with periodontitis. Methods: Genomic DNA was extracted from 107 subjects diagnosed with severe forms of periodontitis to study a cluster of polymorphisms in inflammatory genes, including IL-6. The presence of Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans), Porphyromonas gingivalis, and Tannereila forsythia (previously T. forsythensis) in their subgingival biofilm was determined by polymerase chain reaction analysis. Serum IL-6 and C-reactive protein (CRP) concentrations were analyzed by enzyme-linked immunosorbent assay. Results: Multiple logistic regression analysis revealed that the IL-6 -6106 polymorphism was associated with the detection of A. actinomycetemcomitans (P = 0.009; odds ratio [OR] = 3.5; 95% confidence interval [CI]: 1.38 to 9.16) and the concomitant detection of A. actinomycetemcomitans and P. gingivalis (P = 0.015; OR = 3.6; 95% CI: 1.28 to 10.04). The IL-6 -174 polymorphism was associated with increased odds of the concomitant detection of A. actinomycetemcomitans and P. gingivalis (P = 0.042; OR = 2.8; 95% CI: 1.04 to 7.75). Haplotype analysis of all five IL-6 polymorphisms confirmed an association with the detection of A. actinomycetemcomitans (P = 0.046). The IL-6 -6106 polymorphism was also associated with CRP serum levels at multivariate analysis (P = 0.024). Conclusions: These findings confirm the hypothesis that complex interactions between the microbiota and host genome are at the basis of susceptibility to periodontitis. Periodontal disease may represent a useful model to study the pathways and mechanisms of host-pathogen interactions in inflammatory diseases.-
dc.languageeng-
dc.relation.ispartofJournal of Periodontology-
dc.subjectInterleukin-6-
dc.subjectBacteria-
dc.subjectGenetic polymorphisms-
dc.subjectPeriodontitis-
dc.titleInterleukin-6 polymorphisms are associated with pathogenic bacteria in subjects with periodontitis-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1902/jop.2008.070453-
dc.identifier.pmid18380561-
dc.identifier.scopuseid_2-s2.0-42949098404-
dc.identifier.volume79-
dc.identifier.issue4-
dc.identifier.spage677-
dc.identifier.epage683-

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