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Article: The in vitro effect of different PRP concentrations on osteoblasts and fibroblasts

TitleThe in vitro effect of different PRP concentrations on osteoblasts and fibroblasts
Authors
KeywordsPlatelet-rich plasma
PRP
Osteoprotegerin
Osteoblasts
Ostecalcin
Fibroblasts
Issue Date2006
Citation
Clinical Oral Implants Research, 2006, v. 17, n. 2, p. 212-219 How to Cite?
AbstractObjectives: The aim of this study was to assess the biological rationale for the use of platelet-rich plasma (PRP) by evaluating the effect of different concentrations of PRP on osteoblasts (OB) and fibroblasts (FB) function in vitro. Material and methods: PRP was obtained from volunteer donors using standard protocols. Primary human cultures of oral FBs and OBs were exposed to both activated and non-activated plasma as well as various concentrations of PRP (2.5 ×, 3.5 × and max (4.2-5.5 ×)). Cell proliferation was evaluated after 24 and 72 h using an MTT proliferation assay. Production of osteocalcin (OCN), osteoprotegerin (OPG) and transforming growth factor β1 (TGF-β1) was evaluated in OB after 24 and 72 h. Statistical analysis was performed using one-way ANOVA. Results: PRP-stimulated cell proliferation in both OBs and FBs. The effect of different PRP concentrations on cell proliferation was most notable at 72 h. The maximum effect was achieved with a concentration of 2.5 ×, with higher concentrations resulting in a reduction of cell proliferation. Upregulation of OCN levels and downregulation of OPG levels were noted with increasing PRP concentrations at both 24 and 72 h. TGF-β1 levels were stimulated by increasing concentrations of PRP, with the increased levels being maintained at 72 h. Conclusions: PRP preparations exert a dose-specific effect on oral FBs and OBs. Optimal results were observed at a platelet concentration of 2.5 ×, which was approximately half of the maximal concentrate that could be obtained. Increased concentrations resulted in a reduction in proliferation and a suboptimal effect on OB function. Hence, different PRP concentrations may have an impact on the results that can be obtained in vivo. Copyright © Blackwell Munksgaard 2005.
Persistent Identifierhttp://hdl.handle.net/10722/230760
ISSN
2015 Impact Factor: 3.464
2015 SCImago Journal Rankings: 1.427

 

DC FieldValueLanguage
dc.contributor.authorGraziani, Filippo-
dc.contributor.authorIvanovski, Saso-
dc.contributor.authorCei, Silvia-
dc.contributor.authorDucci, Francesco-
dc.contributor.authorTonetti, Maurizio-
dc.contributor.authorGabriele, Mario-
dc.date.accessioned2016-09-01T06:06:44Z-
dc.date.available2016-09-01T06:06:44Z-
dc.date.issued2006-
dc.identifier.citationClinical Oral Implants Research, 2006, v. 17, n. 2, p. 212-219-
dc.identifier.issn0905-7161-
dc.identifier.urihttp://hdl.handle.net/10722/230760-
dc.description.abstractObjectives: The aim of this study was to assess the biological rationale for the use of platelet-rich plasma (PRP) by evaluating the effect of different concentrations of PRP on osteoblasts (OB) and fibroblasts (FB) function in vitro. Material and methods: PRP was obtained from volunteer donors using standard protocols. Primary human cultures of oral FBs and OBs were exposed to both activated and non-activated plasma as well as various concentrations of PRP (2.5 ×, 3.5 × and max (4.2-5.5 ×)). Cell proliferation was evaluated after 24 and 72 h using an MTT proliferation assay. Production of osteocalcin (OCN), osteoprotegerin (OPG) and transforming growth factor β1 (TGF-β1) was evaluated in OB after 24 and 72 h. Statistical analysis was performed using one-way ANOVA. Results: PRP-stimulated cell proliferation in both OBs and FBs. The effect of different PRP concentrations on cell proliferation was most notable at 72 h. The maximum effect was achieved with a concentration of 2.5 ×, with higher concentrations resulting in a reduction of cell proliferation. Upregulation of OCN levels and downregulation of OPG levels were noted with increasing PRP concentrations at both 24 and 72 h. TGF-β1 levels were stimulated by increasing concentrations of PRP, with the increased levels being maintained at 72 h. Conclusions: PRP preparations exert a dose-specific effect on oral FBs and OBs. Optimal results were observed at a platelet concentration of 2.5 ×, which was approximately half of the maximal concentrate that could be obtained. Increased concentrations resulted in a reduction in proliferation and a suboptimal effect on OB function. Hence, different PRP concentrations may have an impact on the results that can be obtained in vivo. Copyright © Blackwell Munksgaard 2005.-
dc.languageeng-
dc.relation.ispartofClinical Oral Implants Research-
dc.subjectPlatelet-rich plasma-
dc.subjectPRP-
dc.subjectOsteoprotegerin-
dc.subjectOsteoblasts-
dc.subjectOstecalcin-
dc.subjectFibroblasts-
dc.titleThe in vitro effect of different PRP concentrations on osteoblasts and fibroblasts-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1600-0501.2005.01203.x-
dc.identifier.pmid16584418-
dc.identifier.scopuseid_2-s2.0-33644858477-
dc.identifier.volume17-
dc.identifier.issue2-
dc.identifier.spage212-
dc.identifier.epage219-
dc.identifier.eissn1600-0501-

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