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Article: Gene expression profiles of periodontal ligament cells treated with enamel matrix proteins in vitro: Analysis using cDNA arrays

TitleGene expression profiles of periodontal ligament cells treated with enamel matrix proteins in vitro: Analysis using cDNA arrays
Authors
KeywordsPeriodontal ligament
Periodontal regeneration
Wound healing
Enamel matrix derivative
Gene expression
Issue Date2004
Citation
Journal of Periodontology, 2004, v. 75, n. 11, p. 1539-1546 How to Cite?
AbstractBackground: A number of procedures have been used to achieve periodontal regeneration. Recently, enamel matrix derivative (EMD) has been the subject of significant basic and clinical investigations. The precise molecular events involved in EMD modulation of periodontal wound healing are not completely under stood; however, cDNA microarray technology may enable rapid and accurate examination of EMD-mediated changes in gene expression in periodontal ligament (PDL) cells in vitro. The present study was undertaken to explore the selective effects of EMD on the activities of 268 cytokine, growth factor, and receptor genes in PDL. Methods: PDL cells were cultured in the absence and presence of EMD at a concentration of 100 μg/ml for 4 days. RNA was extracted and used to generate labeled cDNA probes. These were hybridized to cDNA arrays comprising 268 genes and exposed to x-ray films. Autoradiographs were digitized and analyzed. Results: Forty-six percent (125 of 268) of the tested genes were found to be expressed by the PDL cells. Of these 125 genes, 38 were differentially expressed by PDL cells which had been cultured in the presence of EMD. Among the 38, 12 were found to be downregulated, notably mostly inflammatory genes, whereas 26 genes demonstrated upregulation, many of these coding for growth factors and growth factor receptors. Conclusions: The present study has shown that EMD downregulates the expression of genes involved in the early inflammatory phases of wound healing while simultaneously upregulating genes encoding growth and repair-promoting molecules. This may partly explain the apparent efficacy of EMD application in periodontal regeneration.
Persistent Identifierhttp://hdl.handle.net/10722/230726
ISSN
2015 Impact Factor: 2.844
2015 SCImago Journal Rankings: 1.070

 

DC FieldValueLanguage
dc.contributor.authorParkar, Mohamed H.-
dc.contributor.authorTonetti, Maurizio-
dc.date.accessioned2016-09-01T06:06:39Z-
dc.date.available2016-09-01T06:06:39Z-
dc.date.issued2004-
dc.identifier.citationJournal of Periodontology, 2004, v. 75, n. 11, p. 1539-1546-
dc.identifier.issn0022-3492-
dc.identifier.urihttp://hdl.handle.net/10722/230726-
dc.description.abstractBackground: A number of procedures have been used to achieve periodontal regeneration. Recently, enamel matrix derivative (EMD) has been the subject of significant basic and clinical investigations. The precise molecular events involved in EMD modulation of periodontal wound healing are not completely under stood; however, cDNA microarray technology may enable rapid and accurate examination of EMD-mediated changes in gene expression in periodontal ligament (PDL) cells in vitro. The present study was undertaken to explore the selective effects of EMD on the activities of 268 cytokine, growth factor, and receptor genes in PDL. Methods: PDL cells were cultured in the absence and presence of EMD at a concentration of 100 μg/ml for 4 days. RNA was extracted and used to generate labeled cDNA probes. These were hybridized to cDNA arrays comprising 268 genes and exposed to x-ray films. Autoradiographs were digitized and analyzed. Results: Forty-six percent (125 of 268) of the tested genes were found to be expressed by the PDL cells. Of these 125 genes, 38 were differentially expressed by PDL cells which had been cultured in the presence of EMD. Among the 38, 12 were found to be downregulated, notably mostly inflammatory genes, whereas 26 genes demonstrated upregulation, many of these coding for growth factors and growth factor receptors. Conclusions: The present study has shown that EMD downregulates the expression of genes involved in the early inflammatory phases of wound healing while simultaneously upregulating genes encoding growth and repair-promoting molecules. This may partly explain the apparent efficacy of EMD application in periodontal regeneration.-
dc.languageeng-
dc.relation.ispartofJournal of Periodontology-
dc.subjectPeriodontal ligament-
dc.subjectPeriodontal regeneration-
dc.subjectWound healing-
dc.subjectEnamel matrix derivative-
dc.subjectGene expression-
dc.titleGene expression profiles of periodontal ligament cells treated with enamel matrix proteins in vitro: Analysis using cDNA arrays-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1902/jop.2004.75.11.1539-
dc.identifier.pmid15633332-
dc.identifier.scopuseid_2-s2.0-10944243836-
dc.identifier.volume75-
dc.identifier.issue11-
dc.identifier.spage1539-
dc.identifier.epage1546-

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