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Conference Paper: Clinical relevance and functional role of galectin-1 in hepatocellular carcinoma

TitleClinical relevance and functional role of galectin-1 in hepatocellular carcinoma
Authors
Issue Date2016
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/ejca
Citation
The 24th Biennial Congress of the European Association for Cancer Research (EACR-24), Manchester, UK., 9-12 July 2016. In European Journal of Cancer, 2016, v. 61 suppl. 1, p. S65, abstract no. 329 How to Cite?
AbstractINTRODUCTION: Hepatocellular carcinoma (HCC) has one the world’s leading cancer incidences and despite extensive research, the numbers of HCC-causing deaths remain high, accounting for the 3rd leading cause of cancer death. Galectins are b-galactose specific binding proteins, which in turn facilitate the crosslinking of molecules and thus enabling vital cell-cell and cell-matrix interactions and signal transduction. Galectin-1 (Gal1), the first identified member of the galectin family, has been found to regulate cell processes; both intracellularly and extracellularly upon its secretion. The oncogenic role of gal1 in HCC has been increasingly studied, however, ambiguities concerning its non-classical secretion mechanism and any associated signalling pathways remain undisclosed. This study aims to further understand the clinical relevance and functional role of Gal1 in HCC tumourigenesis. MATERIALS AND METHODS: Clinical analysis of Gal1 was conducted with human HCC cell lines and patient RNA samples by quantitative real time PCR. ELISA analysis further highlighted the clinical relevance of Gal1 in detecting the secretory gal1 levels in patient blood serum samples. The functional role of Gal1 in HCC was subsequently explored in migration, invasion and soft agar assays after the stable expression of Gal1 in HCC cell lines. RESULTS AND DISCUSSION: Analysis of 48 HCC clinical samples revealed the significantly higher Gal1 expression in HCC late stage tumours compared to early stage. This overexpression was also observed in immunohistological staining of tumour tissues and elevated gal1 levels in patient blood serum samples. Overexpression corresponded to poorer disease-free survival, cirrhotic liver and venous invasion. By knocking down Gal1 expression in HCC cell lines BEL7402 and MHCC97L, functional experiment results showed reduced migration, invasion and anchorage independent growth. Similarly, when Gal1 was overexpressed in HCC cell line PLC/PRF/5, the migratory potential and the ability for anchorage independent growth increased. These results signify the importance of gal1 in regulating essential tumourigenic cell processes. CONCLUSION: Gal1 expression is significantly higher in HCC patient samples observed in the clinical setting and likewise in HCC cell lines. The oncogenic role of Gal1 has been implicated in HCC tumourigenesis by promoting cell migration, invasion and anchorage independent growth.
DescriptionConference Theme: From basic research to precision medicine
Poster Session: Cell and Tumour Biology 1: no. 329
This journal suppl. entitled: 24th Biennial Congress of the European Association for Cancer Research, 9–12 July 2016, Manchester, UK
Persistent Identifierhttp://hdl.handle.net/10722/229930
ISSN
2015 Impact Factor: 6.163
2015 SCImago Journal Rankings: 3.152

 

DC FieldValueLanguage
dc.contributor.authorLeung, Z-
dc.contributor.authorKo, FCF-
dc.contributor.authorYam, JWP-
dc.date.accessioned2016-08-23T14:14:08Z-
dc.date.available2016-08-23T14:14:08Z-
dc.date.issued2016-
dc.identifier.citationThe 24th Biennial Congress of the European Association for Cancer Research (EACR-24), Manchester, UK., 9-12 July 2016. In European Journal of Cancer, 2016, v. 61 suppl. 1, p. S65, abstract no. 329-
dc.identifier.issn0959-8049-
dc.identifier.urihttp://hdl.handle.net/10722/229930-
dc.descriptionConference Theme: From basic research to precision medicine-
dc.descriptionPoster Session: Cell and Tumour Biology 1: no. 329-
dc.descriptionThis journal suppl. entitled: 24th Biennial Congress of the European Association for Cancer Research, 9–12 July 2016, Manchester, UK-
dc.description.abstractINTRODUCTION: Hepatocellular carcinoma (HCC) has one the world’s leading cancer incidences and despite extensive research, the numbers of HCC-causing deaths remain high, accounting for the 3rd leading cause of cancer death. Galectins are b-galactose specific binding proteins, which in turn facilitate the crosslinking of molecules and thus enabling vital cell-cell and cell-matrix interactions and signal transduction. Galectin-1 (Gal1), the first identified member of the galectin family, has been found to regulate cell processes; both intracellularly and extracellularly upon its secretion. The oncogenic role of gal1 in HCC has been increasingly studied, however, ambiguities concerning its non-classical secretion mechanism and any associated signalling pathways remain undisclosed. This study aims to further understand the clinical relevance and functional role of Gal1 in HCC tumourigenesis. MATERIALS AND METHODS: Clinical analysis of Gal1 was conducted with human HCC cell lines and patient RNA samples by quantitative real time PCR. ELISA analysis further highlighted the clinical relevance of Gal1 in detecting the secretory gal1 levels in patient blood serum samples. The functional role of Gal1 in HCC was subsequently explored in migration, invasion and soft agar assays after the stable expression of Gal1 in HCC cell lines. RESULTS AND DISCUSSION: Analysis of 48 HCC clinical samples revealed the significantly higher Gal1 expression in HCC late stage tumours compared to early stage. This overexpression was also observed in immunohistological staining of tumour tissues and elevated gal1 levels in patient blood serum samples. Overexpression corresponded to poorer disease-free survival, cirrhotic liver and venous invasion. By knocking down Gal1 expression in HCC cell lines BEL7402 and MHCC97L, functional experiment results showed reduced migration, invasion and anchorage independent growth. Similarly, when Gal1 was overexpressed in HCC cell line PLC/PRF/5, the migratory potential and the ability for anchorage independent growth increased. These results signify the importance of gal1 in regulating essential tumourigenic cell processes. CONCLUSION: Gal1 expression is significantly higher in HCC patient samples observed in the clinical setting and likewise in HCC cell lines. The oncogenic role of Gal1 has been implicated in HCC tumourigenesis by promoting cell migration, invasion and anchorage independent growth.-
dc.languageeng-
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/ejca-
dc.relation.ispartofEuropean Journal of Cancer-
dc.rightsPosting accepted manuscript (postprint): © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.titleClinical relevance and functional role of galectin-1 in hepatocellular carcinoma-
dc.typeConference_Paper-
dc.identifier.emailKo, FCF: bokcf@hku.hk-
dc.identifier.emailYam, JWP: judyyam@pathology.hku.hk-
dc.identifier.authorityYam, JWP=rp00468-
dc.identifier.doi10.1016/S0959-8049(16)61225-X-
dc.identifier.hkuros261337-
dc.identifier.volume61-
dc.identifier.issuesuppl. 1-
dc.identifier.spageS65, abstract no. 329-
dc.identifier.epageS65, abstract no. 329-
dc.publisher.placeUnited Kingdom-

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