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Conference Paper: Autophagy: a new target for the treatment of salivary gland adenoid cystic carcinoma

TitleAutophagy: a new target for the treatment of salivary gland adenoid cystic carcinoma
Authors
Issue Date2015
PublisherChurchill Livingstone. The Journal's web site is located at http://www.elsevier.com/locate/ijom
Citation
The 22nd International Conference on Oral and Maxillofacial Surgery (ICOMS 2015), Melbourne, Australia, 27-30 October 2015. In International Journal of Oral and Maxillofacial Surgery, 2015, v. 44 suppl. 1, p. e291 How to Cite?
AbstractBACKGROUND: Our previous studies found that expression of Beclin 1 and other autophagy related genes in salivary gland adenoid cystic carcinoma (ACC) was lower than tumour-adjacent normal tissue. Beclin 1 protein and mRNA expression were independent prognostic factors for ACC patients. We hypothesized that autophagy may influence chemosensitivity and radiosensitivity of ACC. OBJECTIVES: To investigate the role of autophagy in radiotherapy and chemotherapy of salivary gland ACC. METHODS: The autophagy level and the expression of Beclin 1 were investigated when ACC cells treated with Obatoclax, Cisplatin, radiation, or Cisplatin+ radiation. Then the cytotoxicity was evaluated when down-regulating or up-regulating expression of Beclin 1, with or without pharmacologically inhibiting autophagy level or apoptosis activity. FINDINGS: Obatoclax increased autophagy level and expression of Beclin 1 in ACC cells. These effects were suppressed by 3-MA or CQ. Autophagy level was decreased when suppressing Beclin 1 expression. When autophagy was suppressed by Beclin 1 shRNA, 3-MA or CQ, sensitivity to Cisplatin of ACC cells was significantly increased. However radiosensitivity of cells decreased when autophagy was suppressed. CONCLUSIONS: Autophagy participates in responding to chemotherapy and radiation of human salivary gland ACC cells. Regulation of autophagy level can influence chemosensitivity and radiosensitivity of ACC cells. These findings indicated regulating of autophagy would be a new therapeutic strategy for salivary gland ACC. The project was supported by Seed Funding Programme for Basic Research, the University of Hong Kong (No. 201501159006).
DescriptionPoster
This journal suppl. entitled: 22nd International Conference on Oral & Maxillofacial Surgery
Persistent Identifierhttp://hdl.handle.net/10722/229742
ISSN
2015 Impact Factor: 1.563
2015 SCImago Journal Rankings: 0.854

 

DC FieldValueLanguage
dc.contributor.authorSu, Y-
dc.contributor.authorLiao, GQ-
dc.contributor.authorLiang, LZ-
dc.contributor.authorMa, B-
dc.contributor.authorOuyang, DQ-
dc.date.accessioned2016-08-23T14:12:59Z-
dc.date.available2016-08-23T14:12:59Z-
dc.date.issued2015-
dc.identifier.citationThe 22nd International Conference on Oral and Maxillofacial Surgery (ICOMS 2015), Melbourne, Australia, 27-30 October 2015. In International Journal of Oral and Maxillofacial Surgery, 2015, v. 44 suppl. 1, p. e291-
dc.identifier.issn0901-5027-
dc.identifier.urihttp://hdl.handle.net/10722/229742-
dc.descriptionPoster-
dc.descriptionThis journal suppl. entitled: 22nd International Conference on Oral & Maxillofacial Surgery-
dc.description.abstractBACKGROUND: Our previous studies found that expression of Beclin 1 and other autophagy related genes in salivary gland adenoid cystic carcinoma (ACC) was lower than tumour-adjacent normal tissue. Beclin 1 protein and mRNA expression were independent prognostic factors for ACC patients. We hypothesized that autophagy may influence chemosensitivity and radiosensitivity of ACC. OBJECTIVES: To investigate the role of autophagy in radiotherapy and chemotherapy of salivary gland ACC. METHODS: The autophagy level and the expression of Beclin 1 were investigated when ACC cells treated with Obatoclax, Cisplatin, radiation, or Cisplatin+ radiation. Then the cytotoxicity was evaluated when down-regulating or up-regulating expression of Beclin 1, with or without pharmacologically inhibiting autophagy level or apoptosis activity. FINDINGS: Obatoclax increased autophagy level and expression of Beclin 1 in ACC cells. These effects were suppressed by 3-MA or CQ. Autophagy level was decreased when suppressing Beclin 1 expression. When autophagy was suppressed by Beclin 1 shRNA, 3-MA or CQ, sensitivity to Cisplatin of ACC cells was significantly increased. However radiosensitivity of cells decreased when autophagy was suppressed. CONCLUSIONS: Autophagy participates in responding to chemotherapy and radiation of human salivary gland ACC cells. Regulation of autophagy level can influence chemosensitivity and radiosensitivity of ACC cells. These findings indicated regulating of autophagy would be a new therapeutic strategy for salivary gland ACC. The project was supported by Seed Funding Programme for Basic Research, the University of Hong Kong (No. 201501159006).-
dc.languageeng-
dc.publisherChurchill Livingstone. The Journal's web site is located at http://www.elsevier.com/locate/ijom-
dc.relation.ispartofInternational Journal of Oral and Maxillofacial Surgery-
dc.rightsPosting accepted manuscript (postprint): © <year>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.titleAutophagy: a new target for the treatment of salivary gland adenoid cystic carcinoma-
dc.typeConference_Paper-
dc.identifier.emailSu, Y: richsu@hku.hk-
dc.identifier.authoritySu, Y=rp01916-
dc.identifier.doi10.1016/j.ijom.2015.08.330-
dc.identifier.hkuros261072-
dc.identifier.hkuros261266-
dc.identifier.volume44-
dc.identifier.issuesuppl. 1-
dc.identifier.spagee291-
dc.identifier.epagee291-
dc.publisher.placeUnited Kingdom-

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