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Article: Regulating the Membrane Transport Activity and Death of Cells via Electroosmotic Manipulation

TitleRegulating the Membrane Transport Activity and Death of Cells via Electroosmotic Manipulation
Authors
Issue Date2016
PublisherCell Press. The Journal's web site is located at http://www.cell.com/biophysj/
Citation
Biophysical Journal, 2016, v. 110, p. 2769-2778 How to Cite?
AbstractAlthough the volume of living cells has been known to heavily influence their behavior and fate, a method allowing us to control the cell size in a programmable manner is still lacking. Here, we develop a technique in which precise changes in the cellular volume can be conveniently introduced by varying the voltage applied across a Nafion membrane that separates the culture medium from a reservoir. It is found that, unlike sudden osmotic shocks, active ion transport across the membrane of leukemia K562 cells will not be triggered by a gradual change in the extracellular osmolarity. Furthermore, when subjected to the same applied voltage, different lung and nasopharyngeal epithelial cancer cells will undergo larger volumetric changes and have a 5-10% higher death rate compared to their normal counterparts. We show that such distinct response is largely caused by the overexpression of aquaporin-4 in tumor cells, with knockout of this water channel protein resulting in a markedly reduced change in the cellular volume. Finally, by taking into account the exchange of water/ion molecules across the Nafion film and the cell membrane, a theoretical model is also proposed to describe the voltage-induced size changes of cells, which explain our experimental observations very well.
Persistent Identifierhttp://hdl.handle.net/10722/229270
ISSN
2015 Impact Factor: 3.632
2015 SCImago Journal Rankings: 2.188
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorHui, THK-
dc.contributor.authorKwan, KW-
dc.contributor.authorYip, TC-
dc.contributor.authorFong, HW-
dc.contributor.authorNgan, KC-
dc.contributor.authorYu, M-
dc.contributor.authorYao, S-
dc.contributor.authorNgan, AHW-
dc.contributor.authorLin, Y-
dc.date.accessioned2016-08-23T14:10:01Z-
dc.date.available2016-08-23T14:10:01Z-
dc.date.issued2016-
dc.identifier.citationBiophysical Journal, 2016, v. 110, p. 2769-2778-
dc.identifier.issn0006-3495-
dc.identifier.urihttp://hdl.handle.net/10722/229270-
dc.description.abstractAlthough the volume of living cells has been known to heavily influence their behavior and fate, a method allowing us to control the cell size in a programmable manner is still lacking. Here, we develop a technique in which precise changes in the cellular volume can be conveniently introduced by varying the voltage applied across a Nafion membrane that separates the culture medium from a reservoir. It is found that, unlike sudden osmotic shocks, active ion transport across the membrane of leukemia K562 cells will not be triggered by a gradual change in the extracellular osmolarity. Furthermore, when subjected to the same applied voltage, different lung and nasopharyngeal epithelial cancer cells will undergo larger volumetric changes and have a 5-10% higher death rate compared to their normal counterparts. We show that such distinct response is largely caused by the overexpression of aquaporin-4 in tumor cells, with knockout of this water channel protein resulting in a markedly reduced change in the cellular volume. Finally, by taking into account the exchange of water/ion molecules across the Nafion film and the cell membrane, a theoretical model is also proposed to describe the voltage-induced size changes of cells, which explain our experimental observations very well.-
dc.languageeng-
dc.publisherCell Press. The Journal's web site is located at http://www.cell.com/biophysj/-
dc.relation.ispartofBiophysical Journal-
dc.rightsPosting accepted manuscript (postprint): © <year>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.titleRegulating the Membrane Transport Activity and Death of Cells via Electroosmotic Manipulation-
dc.typeArticle-
dc.identifier.emailHui, THK: bluesp12@hku.hk-
dc.identifier.emailKwan, KW: kwan15@hku.hk-
dc.identifier.emailNgan, AHW: hwngan@hku.hk-
dc.identifier.emailLin, Y: ylin@hkucc.hku.hk-
dc.identifier.authorityNgan, AHW=rp00225-
dc.identifier.authorityLin, Y=rp00080-
dc.identifier.doi10.1016/j.bpj.2016.05.011-
dc.identifier.pmid27332135-
dc.identifier.pmcidPMC4919594-
dc.identifier.hkuros262552-
dc.identifier.hkuros261874-
dc.identifier.volume110-
dc.identifier.spage2769-
dc.identifier.epage2778-
dc.publisher.placeUnited States-

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