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Article: Extracellular Protease Inhibition Alters the Phenotype of Chondrogenically Differentiating Human Mesenchymal Stem Cells (MSCs) in 3D Collagen Microspheres

TitleExtracellular Protease Inhibition Alters the Phenotype of Chondrogenically Differentiating Human Mesenchymal Stem Cells (MSCs) in 3D Collagen Microspheres
Authors
Issue Date2016
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2016, v. 11 n. 1, p. e0146928 How to Cite?
AbstractMatrix remodeling of cells is highly regulated by proteases and their inhibitors. Nevertheless, how would the chondrogenesis of mesenchymal stem cells (MSCs) be affected, when the balance of the matrix remodeling is disturbed by inhibiting matrix proteases, is incompletely known. Using a previously developed collagen microencapsulation platform, we investigated whether exposing chondrogenically differentiating MSCs to intracellular and extracellular protease inhibitors will affect the extracellular matrix remodeling and hence the outcomes of chondrogenesis. Results showed that inhibition of matrix proteases particularly the extracellular ones favors the phenotype of fibrocartilage rather than hyaline cartilage in chondrogenically differentiating hMSCs by upregulating type I collagen protein deposition and type II collagen gene expression without significantly altering the hypertrophic markers at gene level. This study suggests the potential of manipulating extracellular proteases to alter the outcomes of hMSC chondrogenesis, contributing to future development of differentiation protocols for fibrocartilage tissues for intervertebral disc and meniscus tissue engineering.
Persistent Identifierhttp://hdl.handle.net/10722/227806
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
ISI Accession Number ID
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DC FieldValueLanguage
dc.contributor.authorHan, S-
dc.contributor.authorLin, YY-
dc.contributor.authorChan, BP-
dc.date.accessioned2016-07-19T02:09:42Z-
dc.date.available2016-07-19T02:09:42Z-
dc.date.issued2016-
dc.identifier.citationPlos One, 2016, v. 11 n. 1, p. e0146928-
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10722/227806-
dc.description.abstractMatrix remodeling of cells is highly regulated by proteases and their inhibitors. Nevertheless, how would the chondrogenesis of mesenchymal stem cells (MSCs) be affected, when the balance of the matrix remodeling is disturbed by inhibiting matrix proteases, is incompletely known. Using a previously developed collagen microencapsulation platform, we investigated whether exposing chondrogenically differentiating MSCs to intracellular and extracellular protease inhibitors will affect the extracellular matrix remodeling and hence the outcomes of chondrogenesis. Results showed that inhibition of matrix proteases particularly the extracellular ones favors the phenotype of fibrocartilage rather than hyaline cartilage in chondrogenically differentiating hMSCs by upregulating type I collagen protein deposition and type II collagen gene expression without significantly altering the hypertrophic markers at gene level. This study suggests the potential of manipulating extracellular proteases to alter the outcomes of hMSC chondrogenesis, contributing to future development of differentiation protocols for fibrocartilage tissues for intervertebral disc and meniscus tissue engineering.-
dc.languageeng-
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action-
dc.relation.ispartofPlos One-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleExtracellular Protease Inhibition Alters the Phenotype of Chondrogenically Differentiating Human Mesenchymal Stem Cells (MSCs) in 3D Collagen Microspheres-
dc.typeArticle-
dc.identifier.emailChan, BP: bpchan@hkucc.hku.hk-
dc.identifier.authorityChan, BP=rp00087-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0146928-
dc.identifier.pmid26760956-
dc.identifier.volume11-
dc.identifier.issue1-
dc.identifier.spagee0146928-
dc.identifier.epagee0146928-
dc.identifier.isiWOS:000368033100054-
dc.publisher.placeUnited States-
dc.relation.projectBioengineering a human mesenchymal stem cell-based osteochondral construct with calcified cartilage interface for cartilage repair-
dc.relation.projectDevelopment of a human mesenchymal stem cell (MSC)-based tri-layered osteochondral graft for osteoarthritis (OA) treatment-

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