File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL
Supplementary

Conference Paper: Cellular internalization and epithelial penetration of fluorescent-labeled mesoporous silica nanoparticles

TitleCellular internalization and epithelial penetration of fluorescent-labeled mesoporous silica nanoparticles
Authors
Issue Date2016
PublisherSage Publications, Inc. The Journal's web site is located at http://jdr.sagepub.com/
Citation
The 94th General Session & Exhibition of the IADR, 3rd Meeting of the IADR Asia Pacific Region & 35th Annual Meeting of the IADR Korean Division, Seoul, Korea, 22-25 June 2016. In Journal of Dental Research, 2016, v. 95 Spec. Iss. B, abstract no. 2022 How to Cite?
AbstractOBJECTIVES: Understanding of cellular internalization and epithelial penetration capacity is crucial to develop the nano-vehicles for drug application in dentistry. This study investigated the cellular uptake, internalization and tissue penetration of our newly synthesized fluorescent spherical mesoporous silica nanoparticles (S-MSNs). METHODS: The fabricated MSNs were surface-modified with amine groups, and subsequently labeled with rhodamine B isothiocyanate (RITC-NP). The RITC-NP fluorescence was detected by fluorescence spectrometer, and the RITC-NP cytotoxicity was evaluated in primary human gingival epithelial cells (HGECs) and differentiated THP-1 cells using CCK-8 kit. Meanwhile, the cellular uptake of RITC-NP was accessed by the confocal scanning laser microscopy after 24h of treatment. Furthermore, the isolated porcine ear skins and reconstituted human gingival epithelia (RHGE) were treated with 200 μg/mL of RITC-NP for 2, 6 and 24 h, and then fixed in 4% paraformaldehyde. The tissue cryosections were stained with DAPI, and subsequently the fluorescence-labeled MSNs were assessed by the fluorescent microscope. The H&E stain was conducted on the cryosections for histological analysis. RESULTS: The RITC-NP (average size of 250 nm) with shifted emission wavelength at 580 nm exhibited a relatively low cytotoxicity on the cells. These labeled-MSNs were mainly distributed around the cellular nuclei after 24 h of treatment. Moreover, the RITC-NP began to penetrate the corneum of porcine ear skin after 2 h of treatment, and notably these particles could be detected in the deep layers of epithelia at 24 h. Interestingly, the RITC-NP accumulated merely in the corneum layer of RHGE during the 24h experiment without causing tissue damage. CONCLUSIONS: This pioneering study demonstrates the penetration and biodistribution of the MSNs in both cells and tissues. It may facilitate further development of nano-based drug delivery for clinical application.
DescriptionPoster Session - Antimicrobial & Host Modulating Approaches to Treat Periodontal Diseases: no. 2022
Persistent Identifierhttp://hdl.handle.net/10722/227495
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorLi, X-
dc.contributor.authorPang, KY-
dc.contributor.authorLeung, KCF-
dc.contributor.authorJin, L-
dc.date.accessioned2016-07-18T09:11:03Z-
dc.date.available2016-07-18T09:11:03Z-
dc.date.issued2016-
dc.identifier.citationThe 94th General Session & Exhibition of the IADR, 3rd Meeting of the IADR Asia Pacific Region & 35th Annual Meeting of the IADR Korean Division, Seoul, Korea, 22-25 June 2016. In Journal of Dental Research, 2016, v. 95 Spec. Iss. B, abstract no. 2022-
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/227495-
dc.descriptionPoster Session - Antimicrobial & Host Modulating Approaches to Treat Periodontal Diseases: no. 2022-
dc.description.abstractOBJECTIVES: Understanding of cellular internalization and epithelial penetration capacity is crucial to develop the nano-vehicles for drug application in dentistry. This study investigated the cellular uptake, internalization and tissue penetration of our newly synthesized fluorescent spherical mesoporous silica nanoparticles (S-MSNs). METHODS: The fabricated MSNs were surface-modified with amine groups, and subsequently labeled with rhodamine B isothiocyanate (RITC-NP). The RITC-NP fluorescence was detected by fluorescence spectrometer, and the RITC-NP cytotoxicity was evaluated in primary human gingival epithelial cells (HGECs) and differentiated THP-1 cells using CCK-8 kit. Meanwhile, the cellular uptake of RITC-NP was accessed by the confocal scanning laser microscopy after 24h of treatment. Furthermore, the isolated porcine ear skins and reconstituted human gingival epithelia (RHGE) were treated with 200 μg/mL of RITC-NP for 2, 6 and 24 h, and then fixed in 4% paraformaldehyde. The tissue cryosections were stained with DAPI, and subsequently the fluorescence-labeled MSNs were assessed by the fluorescent microscope. The H&E stain was conducted on the cryosections for histological analysis. RESULTS: The RITC-NP (average size of 250 nm) with shifted emission wavelength at 580 nm exhibited a relatively low cytotoxicity on the cells. These labeled-MSNs were mainly distributed around the cellular nuclei after 24 h of treatment. Moreover, the RITC-NP began to penetrate the corneum of porcine ear skin after 2 h of treatment, and notably these particles could be detected in the deep layers of epithelia at 24 h. Interestingly, the RITC-NP accumulated merely in the corneum layer of RHGE during the 24h experiment without causing tissue damage. CONCLUSIONS: This pioneering study demonstrates the penetration and biodistribution of the MSNs in both cells and tissues. It may facilitate further development of nano-based drug delivery for clinical application.-
dc.languageeng-
dc.publisherSage Publications, Inc. The Journal's web site is located at http://jdr.sagepub.com/-
dc.relation.ispartofJournal of Dental Research-
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.-
dc.titleCellular internalization and epithelial penetration of fluorescent-labeled mesoporous silica nanoparticles-
dc.typeConference_Paper-
dc.identifier.emailPang, KY: pangky@HKUCC-COM.hku.hk-
dc.identifier.emailJin, L: ljjin@hkucc.hku.hk-
dc.identifier.authorityJin, L=rp00028-
dc.identifier.hkuros259725-
dc.identifier.volume95-
dc.identifier.issueSpec. Iss. B-
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats