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Conference Paper: Salivary interleukin-1β response is associated with microbiome diversity

TitleSalivary interleukin-1β response is associated with microbiome diversity
Authors
Issue Date2016
PublisherSage Publications, Inc. The Journal's web site is located at http://jdr.sagepub.com/
Citation
The 94th General Session & Exhibition of the IADR, 3rd Meeting of the IADR Asia Pacific Region & 35th Annual Meeting of the IADR Korean Division, Seoul, Korea, 22-25 June 2016. In Journal of Dental Research, 2016, v. 95 Spec. Iss. B, abstract no. 1247 How to Cite?
AbstractOBJECTIVES: The study investigated the association of the salivary microbiome diversity with salivary Interleukin- 1 β (IL-1β) levels. METHODS: 90 systemically healthy subjects’ under supportive dental care were included. Unstimulated whole saliva, clinical and demographic parameters including history of chronic periodontitis were recorded. 16SrRNA V3-V4 gene amplicons from salivary DNA were subjected to Illumina MISEQ 300 base paired-end sequencing. Data was processed in the Qiime pipeline to generate an OTU table and normalized for differences in read counts per sample. Alpha and beta microbial diversity measures were generated. Mann Whitney U tests for variation of IL- 1β distribution with clinical measures, multiple alpha diversity measures’ (Chao1, Phylogenetic diversity, Shannon) correlation with IL- 1β (Spearman’s rank correlation coefficient) was analyzed. Percentage variation in IL- 1β effected by global community composition was analyzed by relating with weighted Unifrac distance beta metrics using Adonis, a nonparametric statistical method. RESULTS: Salivary IL- 1β distribution did not vary significantly with past history of chronic periodontitis (p= 0.596, Mann-Whitney U test). 4187694 non-chimeric 16SrRNA sequences were clustered into 5238 OTUs and mapped to 301 unique taxa. Alpha diversity metrics; Chao1 (r=-0.293,p=0.009), Phylogenetic diversity (r =-0.333, p=0.001) Shannon (r=-0.282, p=0.007) were significantly negatively correlated with salivary IL- 1β levels. Weighted Unifrac beta diversity metric was significantly associated with IL- 1β (p= 0.4296). CONCLUSIONS: Decreased richness and phylogenetic diversity of the salivary microbiome was linked to greater salivary IL- 1β levels in subjects under supportive dental care. The study demonstrated that differences in community composition and a richer salivary microbiome seem to associate with a healthier host physiologic status.
DescriptionPoster Session - Salivary Research III: no. 1247
Persistent Identifierhttp://hdl.handle.net/10722/227494
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorAcharya, A-
dc.contributor.authorKheur, S-
dc.contributor.authorGopalakrishnan, D-
dc.contributor.authorWatt, RM-
dc.contributor.authorJin, L-
dc.contributor.authorMattheos, N-
dc.date.accessioned2016-07-18T09:11:03Z-
dc.date.available2016-07-18T09:11:03Z-
dc.date.issued2016-
dc.identifier.citationThe 94th General Session & Exhibition of the IADR, 3rd Meeting of the IADR Asia Pacific Region & 35th Annual Meeting of the IADR Korean Division, Seoul, Korea, 22-25 June 2016. In Journal of Dental Research, 2016, v. 95 Spec. Iss. B, abstract no. 1247-
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/227494-
dc.descriptionPoster Session - Salivary Research III: no. 1247-
dc.description.abstractOBJECTIVES: The study investigated the association of the salivary microbiome diversity with salivary Interleukin- 1 β (IL-1β) levels. METHODS: 90 systemically healthy subjects’ under supportive dental care were included. Unstimulated whole saliva, clinical and demographic parameters including history of chronic periodontitis were recorded. 16SrRNA V3-V4 gene amplicons from salivary DNA were subjected to Illumina MISEQ 300 base paired-end sequencing. Data was processed in the Qiime pipeline to generate an OTU table and normalized for differences in read counts per sample. Alpha and beta microbial diversity measures were generated. Mann Whitney U tests for variation of IL- 1β distribution with clinical measures, multiple alpha diversity measures’ (Chao1, Phylogenetic diversity, Shannon) correlation with IL- 1β (Spearman’s rank correlation coefficient) was analyzed. Percentage variation in IL- 1β effected by global community composition was analyzed by relating with weighted Unifrac distance beta metrics using Adonis, a nonparametric statistical method. RESULTS: Salivary IL- 1β distribution did not vary significantly with past history of chronic periodontitis (p= 0.596, Mann-Whitney U test). 4187694 non-chimeric 16SrRNA sequences were clustered into 5238 OTUs and mapped to 301 unique taxa. Alpha diversity metrics; Chao1 (r=-0.293,p=0.009), Phylogenetic diversity (r =-0.333, p=0.001) Shannon (r=-0.282, p=0.007) were significantly negatively correlated with salivary IL- 1β levels. Weighted Unifrac beta diversity metric was significantly associated with IL- 1β (p= 0.4296). CONCLUSIONS: Decreased richness and phylogenetic diversity of the salivary microbiome was linked to greater salivary IL- 1β levels in subjects under supportive dental care. The study demonstrated that differences in community composition and a richer salivary microbiome seem to associate with a healthier host physiologic status.-
dc.languageeng-
dc.publisherSage Publications, Inc. The Journal's web site is located at http://jdr.sagepub.com/-
dc.relation.ispartofJournal of Dental Research-
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.-
dc.titleSalivary interleukin-1β response is associated with microbiome diversity-
dc.typeConference_Paper-
dc.identifier.emailWatt, RM: rmwatt@hku.hk-
dc.identifier.emailJin, L: ljjin@hkucc.hku.hk-
dc.identifier.emailMattheos, N: mattheos@hku.hk-
dc.identifier.authorityWatt, RM=rp00043-
dc.identifier.authorityJin, L=rp00028-
dc.identifier.authorityMattheos, N=rp01662-
dc.identifier.hkuros259724-
dc.identifier.volume95-
dc.identifier.issueSpec. Iss. B-
dc.publisher.placeUnited States-

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