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Article: A Mass Spectrometric-Derived Cell Surface Protein Atlas

TitleA Mass Spectrometric-Derived Cell Surface Protein Atlas
Authors
Issue Date2015
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2015, v. 10 n. 4, p. e0121314 How to Cite?
AbstractCell surface proteins are major targets of biomedical research due to their utility as cellular markers and their extracellular accessibility for pharmacological intervention. However, information about the cell surface protein repertoire (the surfaceome) of individual cells is only sparsely available. Here, we applied the Cell Surface Capture (CSC) technology to 41 human and 31 mouse cell types to generate a mass-spectrometry derived Cell Surface Protein Atlas (CSPA) providing cellular surfaceome snapshots at high resolution. The CSPA is presented in form of an easy-to-navigate interactive database, a downloadable data matrix and with tools for targeted surfaceome rediscovery (http://wlab.ethz.ch/cspa). The cellular surfaceome snapshots of different cell types, including cancer cells, resulted in a combined dataset of 1492 human and 1296 mouse cell surface glycoproteins, providing experimental evidence for their cell surface expression on different cell types, including 136 G-protein coupled receptors and 75 membrane receptor tyrosine-protein kinases. Integrated analysis of the CSPA reveals that the concerted biological function of individual cell types is mainly guided by quantitative rather than qualitative surfaceome differences. The CSPA will be useful for the evaluation of drug targets, for the improved classification of cell types and for a better understanding of the surfaceome and its concerted biological functions in complex signaling microenvironments.
Persistent Identifierhttp://hdl.handle.net/10722/227011
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBausch-Fluck, D-
dc.contributor.authorHofmann, A-
dc.contributor.authorBock, T-
dc.contributor.authorFrei, AP-
dc.contributor.authorCerciello, F-
dc.contributor.authorJacobs, A-
dc.contributor.authorMoest, H-
dc.contributor.authorOmasits, U-
dc.contributor.authorGundry, RL-
dc.contributor.authorYoon, C-
dc.contributor.authorSchiess, R-
dc.contributor.authorSchmidt, A-
dc.contributor.authorMirkowska, P-
dc.contributor.authorHärtlová, A-
dc.contributor.authorVan Eyk, JE-
dc.contributor.authorBourquin, JP-
dc.contributor.authorAebersold, R-
dc.contributor.authorBoheler, KR-
dc.contributor.authorZandstra, P-
dc.contributor.authorWollscheid, B-
dc.date.accessioned2016-07-14T04:59:10Z-
dc.date.available2016-07-14T04:59:10Z-
dc.date.issued2015-
dc.identifier.citationPlos One, 2015, v. 10 n. 4, p. e0121314-
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10722/227011-
dc.description.abstractCell surface proteins are major targets of biomedical research due to their utility as cellular markers and their extracellular accessibility for pharmacological intervention. However, information about the cell surface protein repertoire (the surfaceome) of individual cells is only sparsely available. Here, we applied the Cell Surface Capture (CSC) technology to 41 human and 31 mouse cell types to generate a mass-spectrometry derived Cell Surface Protein Atlas (CSPA) providing cellular surfaceome snapshots at high resolution. The CSPA is presented in form of an easy-to-navigate interactive database, a downloadable data matrix and with tools for targeted surfaceome rediscovery (http://wlab.ethz.ch/cspa). The cellular surfaceome snapshots of different cell types, including cancer cells, resulted in a combined dataset of 1492 human and 1296 mouse cell surface glycoproteins, providing experimental evidence for their cell surface expression on different cell types, including 136 G-protein coupled receptors and 75 membrane receptor tyrosine-protein kinases. Integrated analysis of the CSPA reveals that the concerted biological function of individual cell types is mainly guided by quantitative rather than qualitative surfaceome differences. The CSPA will be useful for the evaluation of drug targets, for the improved classification of cell types and for a better understanding of the surfaceome and its concerted biological functions in complex signaling microenvironments.-
dc.languageeng-
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action-
dc.relation.ispartofPlos One-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleA Mass Spectrometric-Derived Cell Surface Protein Atlas-
dc.typeArticle-
dc.identifier.emailBoheler, KR: bohelerk@hku.hk-
dc.identifier.authorityBoheler, KR=rp01884-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0121314-
dc.identifier.pmid25894527-
dc.identifier.volume10-
dc.identifier.issue4-
dc.identifier.spagee0121314-
dc.identifier.epagee0121314-
dc.identifier.isiWOS:000353211700009-
dc.publisher.placeUnited States-

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