File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The function of mitochondria in presynaptic development at the neuromuscular junction

TitleThe function of mitochondria in presynaptic development at the neuromuscular junction
Authors
Issue Date2008
PublisherAmerican Society for Cell Biology. The Journal's web site is located at http://www.molbiolcell.org/
Citation
Molecular Biology of the Cell, 2008, v. 19 n. 1, p. 150-158 How to Cite?
AbstractMitochondria with high membrane potential (ΔΨm) are enriched in the presynaptic nerve terminal at vertebrate neuromuscular junctions, but the exact function of these localized synaptic mitochondria remains unclear. Here, we investigated the correlation between mitochondrial ΔΨm and the development of synaptic specializations. Using mitochondrial ΔΨm-sensitive probe JC-1, we found that ΔΨm in Xenopus spinal neurons could be reversibly elevated by creatine and suppressed by FCCP. Along naïve neurites, preexisting synaptic vesicle (SV) clusters were positively correlated with mitochondrial ΔΨm, suggesting a potential regulatory role of mitochondrial activity in synaptogenesis. Indicating a specific role of mitochondrial activity in presynaptic development, mitochondrial ATP synthase inhibitor oligomycin, but not mitochondrial Na+/Ca2+ exchanger inhibitor CGP-37157, inhibited the clustering of SVs induced by growth factor–coated beads. Local F-actin assembly induced along spinal neurites by beads was suppressed by FCCP or oligomycin. Our results suggest that a key role of presynaptic mitochondria is to provide ATP for the assembly of actin cytoskeleton involved in the assembly of the presynaptic specialization including the clustering of SVs and mitochondria themselves.
Persistent Identifierhttp://hdl.handle.net/10722/225409
ISSN
2015 Impact Factor: 4.037
2015 SCImago Journal Rankings: 3.665
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorLee, CW-
dc.contributor.authorPeng, HB-
dc.date.accessioned2016-05-06T01:50:19Z-
dc.date.available2016-05-06T01:50:19Z-
dc.date.issued2008-
dc.identifier.citationMolecular Biology of the Cell, 2008, v. 19 n. 1, p. 150-158-
dc.identifier.issn1059-1524-
dc.identifier.urihttp://hdl.handle.net/10722/225409-
dc.description.abstractMitochondria with high membrane potential (ΔΨm) are enriched in the presynaptic nerve terminal at vertebrate neuromuscular junctions, but the exact function of these localized synaptic mitochondria remains unclear. Here, we investigated the correlation between mitochondrial ΔΨm and the development of synaptic specializations. Using mitochondrial ΔΨm-sensitive probe JC-1, we found that ΔΨm in Xenopus spinal neurons could be reversibly elevated by creatine and suppressed by FCCP. Along naïve neurites, preexisting synaptic vesicle (SV) clusters were positively correlated with mitochondrial ΔΨm, suggesting a potential regulatory role of mitochondrial activity in synaptogenesis. Indicating a specific role of mitochondrial activity in presynaptic development, mitochondrial ATP synthase inhibitor oligomycin, but not mitochondrial Na+/Ca2+ exchanger inhibitor CGP-37157, inhibited the clustering of SVs induced by growth factor–coated beads. Local F-actin assembly induced along spinal neurites by beads was suppressed by FCCP or oligomycin. Our results suggest that a key role of presynaptic mitochondria is to provide ATP for the assembly of actin cytoskeleton involved in the assembly of the presynaptic specialization including the clustering of SVs and mitochondria themselves.-
dc.languageeng-
dc.publisherAmerican Society for Cell Biology. The Journal's web site is located at http://www.molbiolcell.org/-
dc.relation.ispartofMolecular Biology of the Cell-
dc.rightsMolecular Biology of the Cell. Copyright © American Society for Cell Biology.-
dc.titleThe function of mitochondria in presynaptic development at the neuromuscular junction-
dc.typeArticle-
dc.identifier.emailLee, CW: chiwai.lee@hku.hk-
dc.identifier.authorityLee, CW=rp02089-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1091/mbc.E07-05-0515-
dc.identifier.pmcidPMC2174173-
dc.identifier.volume19-
dc.identifier.issue1-
dc.identifier.spage150-
dc.identifier.epage158-
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats