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Article: The constitutive activity of the ghrelin receptor attenuates apoptosis via a protein kinase C-dependent pathway

TitleThe constitutive activity of the ghrelin receptor attenuates apoptosis via a protein kinase C-dependent pathway
Authors
KeywordsConstitutive activity
Apoptosis
Ghrelin receptors
Issue Date2009
Citation
Molecular and Cellular Endocrinology, 2009, v. 299, n. 2, p. 232-239 How to Cite?
AbstractThe ghrelin receptor (GHS-R1a) displays a high level of constitutive signaling through a phospholipase C/protein kinase C-dependent pathway. Therefore, we have investigated the role of agonist-dependent and agonist-independent signaling of GHS-R1a in apoptosis using the seabream GHS-R1a stably expressed in human embryonic kidney 293 cells (HEK-sbGHS-R1a cells). Cadmium-induced activation of caspase-3 was significantly attenuated in HEK-sbGHS-R1a cells compared to wild-type HEK293 cells, while the apoptotic responses to the protein kinase C inhibitor staurosporine were similar. GHS-R1a ligands had no effect on caspase-3 activation or on cell proliferation. Concentrations of the inverse agonist [d-Arg1,d-Phe5,d-Trp7,9,Leu11]-substance P sufficient to inhibit constitutive inositol phosphate generation did not enhance caspase-3 activity, suggesting a possible role of phosphatidylcholine-specific phospholipase C in the anti-apoptotic activity of GHS-R1a. In conclusion, our data suggests that the constitutive activity of sbGHS-R1a may be sufficient alone to attenuate apoptosis via a protein kinase C-dependent pathway. © 2008 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/225047
ISSN
2015 Impact Factor: 3.859
2015 SCImago Journal Rankings: 2.116

 

DC FieldValueLanguage
dc.contributor.authorLau, Pui Ngan-
dc.contributor.authorChow, Kevin B S-
dc.contributor.authorChan, Chi Bun-
dc.contributor.authorCheng, Christopher H K-
dc.contributor.authorWise, Helen-
dc.date.accessioned2016-04-18T11:16:37Z-
dc.date.available2016-04-18T11:16:37Z-
dc.date.issued2009-
dc.identifier.citationMolecular and Cellular Endocrinology, 2009, v. 299, n. 2, p. 232-239-
dc.identifier.issn0303-7207-
dc.identifier.urihttp://hdl.handle.net/10722/225047-
dc.description.abstractThe ghrelin receptor (GHS-R1a) displays a high level of constitutive signaling through a phospholipase C/protein kinase C-dependent pathway. Therefore, we have investigated the role of agonist-dependent and agonist-independent signaling of GHS-R1a in apoptosis using the seabream GHS-R1a stably expressed in human embryonic kidney 293 cells (HEK-sbGHS-R1a cells). Cadmium-induced activation of caspase-3 was significantly attenuated in HEK-sbGHS-R1a cells compared to wild-type HEK293 cells, while the apoptotic responses to the protein kinase C inhibitor staurosporine were similar. GHS-R1a ligands had no effect on caspase-3 activation or on cell proliferation. Concentrations of the inverse agonist [d-Arg1,d-Phe5,d-Trp7,9,Leu11]-substance P sufficient to inhibit constitutive inositol phosphate generation did not enhance caspase-3 activity, suggesting a possible role of phosphatidylcholine-specific phospholipase C in the anti-apoptotic activity of GHS-R1a. In conclusion, our data suggests that the constitutive activity of sbGHS-R1a may be sufficient alone to attenuate apoptosis via a protein kinase C-dependent pathway. © 2008 Elsevier Ireland Ltd. All rights reserved.-
dc.languageeng-
dc.relation.ispartofMolecular and Cellular Endocrinology-
dc.subjectConstitutive activity-
dc.subjectApoptosis-
dc.subjectGhrelin receptors-
dc.titleThe constitutive activity of the ghrelin receptor attenuates apoptosis via a protein kinase C-dependent pathway-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.mce.2008.12.006-
dc.identifier.pmid19135127-
dc.identifier.scopuseid_2-s2.0-58649097003-
dc.identifier.volume299-
dc.identifier.issue2-
dc.identifier.spage232-
dc.identifier.epage239-

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