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Conference Paper: Development of osteoarthritis-like changes in transgenic endothelin-1 over-expressed mice

TitleDevelopment of osteoarthritis-like changes in transgenic endothelin-1 over-expressed mice
Authors
Issue Date2013
PublisherHong Kong Academy of Medicine Press.
Citation
The 33rd Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, 23-24 November 2013, p. 17 Abstract no. 2.2 How to Cite?
AbstractIntroduction: Hypertension and type 2 diabetes mellitus are frequently encountered in knee osteoarthritis (OA) patients at the age of 65 years and above. Endothelin-1 (ET-1), known as a potent vasoconstrictor, has been implicated in pathogenesis of essential hypertension and diabetic vascular complications. High level of ET-1 was observed in osteoarthritic cartilage. The unanswered question was whether the sustained high level of ET-1 would induce development of OA. This study aimed to investigate the phenotypes of articular cartilage and subchondral bone in transgenic mice with endothelial cellspecific overexpression of ET-1 (TET-1 mice). Materials and Methods: Male heterozygous TET-1 mice were generated by microinjection of the ET-1 construct driven by Tie-1 promoter. These TET-1 mice developed systemic hypertension with altered vascular reactivity since 8 weeks after birth. Tibiae of male, heterozygous TET-1 mice (n = 4) and their littermates (n = 4) of 35-week-old were obtained. Microcomputed tomographic (Micro-CT) scan on tibiae were performed before tissue processed to wax blocks. Tibiae in wax blocks were sectioned for histology. Results: The Micro-CT data showed a decrease of mean (± standard deviation) trabecular bone density (10.9 ± 0.4%) in primary spongiosa of TET-1 mice compared with the littermates (12.8 ± 0.5%; p < 0.05). Histologically, articular chondrocytes underwent hypertrophic changes together with thickening of calcified cartilage in TET-1 mice when compared with their littermates. Discussion and Conclusion: Endothelial-specific overexpression of ET-1 induced OA-like changes, which might be a therapeutic target.
Persistent Identifierhttp://hdl.handle.net/10722/224627

 

DC FieldValueLanguage
dc.contributor.authorWen, C-
dc.contributor.authorOliver, B-
dc.contributor.authorChung, SK-
dc.contributor.authorXu, AM-
dc.contributor.authorYan, CH-
dc.contributor.authorChiu, PKY-
dc.date.accessioned2016-04-11T09:32:45Z-
dc.date.available2016-04-11T09:32:45Z-
dc.date.issued2013-
dc.identifier.citationThe 33rd Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, 23-24 November 2013, p. 17 Abstract no. 2.2-
dc.identifier.urihttp://hdl.handle.net/10722/224627-
dc.description.abstractIntroduction: Hypertension and type 2 diabetes mellitus are frequently encountered in knee osteoarthritis (OA) patients at the age of 65 years and above. Endothelin-1 (ET-1), known as a potent vasoconstrictor, has been implicated in pathogenesis of essential hypertension and diabetic vascular complications. High level of ET-1 was observed in osteoarthritic cartilage. The unanswered question was whether the sustained high level of ET-1 would induce development of OA. This study aimed to investigate the phenotypes of articular cartilage and subchondral bone in transgenic mice with endothelial cellspecific overexpression of ET-1 (TET-1 mice). Materials and Methods: Male heterozygous TET-1 mice were generated by microinjection of the ET-1 construct driven by Tie-1 promoter. These TET-1 mice developed systemic hypertension with altered vascular reactivity since 8 weeks after birth. Tibiae of male, heterozygous TET-1 mice (n = 4) and their littermates (n = 4) of 35-week-old were obtained. Microcomputed tomographic (Micro-CT) scan on tibiae were performed before tissue processed to wax blocks. Tibiae in wax blocks were sectioned for histology. Results: The Micro-CT data showed a decrease of mean (± standard deviation) trabecular bone density (10.9 ± 0.4%) in primary spongiosa of TET-1 mice compared with the littermates (12.8 ± 0.5%; p < 0.05). Histologically, articular chondrocytes underwent hypertrophic changes together with thickening of calcified cartilage in TET-1 mice when compared with their littermates. Discussion and Conclusion: Endothelial-specific overexpression of ET-1 induced OA-like changes, which might be a therapeutic target.-
dc.languageeng-
dc.publisherHong Kong Academy of Medicine Press. -
dc.relation.ispartofAbstracts of the 33rd Annual Congress of the Hong Kong Orthopaedic Association-
dc.rightsAbstracts of the 33rd Annual Congress of the Hong Kong Orthopaedic Association. Copyright © Hong Kong Academy of Medicine Press.-
dc.titleDevelopment of osteoarthritis-like changes in transgenic endothelin-1 over-expressed mice-
dc.typeConference_Paper-
dc.identifier.emailWen, C: paulwen@hku.hk-
dc.identifier.emailYan, CH: yanchoi@hku.hk-
dc.identifier.emailChiu, PKY: pkychiu@hkucc.hku.hk-
dc.identifier.authorityYan, CH=rp00303-
dc.identifier.authorityChiu, PKY=rp00379-
dc.identifier.hkuros240256-
dc.identifier.spage17 Abstract no. 2.2-
dc.identifier.epage17 Abstract no. 2.2-
dc.publisher.placeHong Kong-

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