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Conference Paper: Profile of metronidazole resistance among subgingival anaerobic bacteria

TitleProfile of metronidazole resistance among subgingival anaerobic bacteria
Authors
KeywordsPeriodontics
Plaque
Bacterial
Antimicrobials
Antimicrobial agents/inhibitors
Issue Date2010
PublisherSage Publications, Inc. The Journal's web site is located at http://jdr.sagepub.com/
Citation
The 24th IADR-SEA Division Annual Scientific Meeting, Taipei, Taiwan, 19-21 September 2010. In Journal of Dental Research, 2010, v. 89 n. Spec Iss C, p. Abstract no. 104 How to Cite?
AbstractObjectives: Metronidazole resistance among subgingival anaerobic bacteria has been demonstrated phenotypically and genotypically in various populations. This study aimed to screen for metronidazole resistance among anaerobic clinical culture collection from human subgingival plaque in a southern Chinese population and to detect the prevalence of nimA gene of the metronidazole resistant strains. Methods: 47 subgingival periodontal isolates were cultured on blood agar and incubated anaerobically. Pure colonies were used for antimicrobial susceptibility testing by broth microdilution in metronidazole concentrations of 0.25-to-512µg/ml. The presence of nimA gene in resistance strains as determined using PCR method. Identification of metronidazole resistant strains was confirmed by 16SrDNA sequencing. Results: Majority of presumptive identified Fusobacterium spp. (7/10) and Propionibacterium spp. (8/9) were found to be highly resistant to metronidazole (>128 µg/ml). A few of Bacteroides spp. (5/17) were found to be metronidazole resistant. Nearly all of Peptostreptococcus spp. (6/7) and Prevotella spp. (3/4) were susceptible to metronidazole. None of the resistance strains were positive upon PCR-based nimA gene detection. Conclusion: Fusobacterium and Propionibacterium from subgingival periodontal isolates were found to be highly resistant to metronidazole. However, none of these resistant strains were found to possess nimA gene. Reasons for metronidazole resistance in these clinical isolates, remains to be determined.
Persistent Identifierhttp://hdl.handle.net/10722/224278
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorRamli, H-
dc.contributor.authorLeung, WK-
dc.contributor.authorWatt, RM-
dc.contributor.authorCorbet, EF-
dc.date.accessioned2016-03-31T02:43:42Z-
dc.date.available2016-03-31T02:43:42Z-
dc.date.issued2010-
dc.identifier.citationThe 24th IADR-SEA Division Annual Scientific Meeting, Taipei, Taiwan, 19-21 September 2010. In Journal of Dental Research, 2010, v. 89 n. Spec Iss C, p. Abstract no. 104-
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/224278-
dc.description.abstractObjectives: Metronidazole resistance among subgingival anaerobic bacteria has been demonstrated phenotypically and genotypically in various populations. This study aimed to screen for metronidazole resistance among anaerobic clinical culture collection from human subgingival plaque in a southern Chinese population and to detect the prevalence of nimA gene of the metronidazole resistant strains. Methods: 47 subgingival periodontal isolates were cultured on blood agar and incubated anaerobically. Pure colonies were used for antimicrobial susceptibility testing by broth microdilution in metronidazole concentrations of 0.25-to-512µg/ml. The presence of nimA gene in resistance strains as determined using PCR method. Identification of metronidazole resistant strains was confirmed by 16SrDNA sequencing. Results: Majority of presumptive identified Fusobacterium spp. (7/10) and Propionibacterium spp. (8/9) were found to be highly resistant to metronidazole (>128 µg/ml). A few of Bacteroides spp. (5/17) were found to be metronidazole resistant. Nearly all of Peptostreptococcus spp. (6/7) and Prevotella spp. (3/4) were susceptible to metronidazole. None of the resistance strains were positive upon PCR-based nimA gene detection. Conclusion: Fusobacterium and Propionibacterium from subgingival periodontal isolates were found to be highly resistant to metronidazole. However, none of these resistant strains were found to possess nimA gene. Reasons for metronidazole resistance in these clinical isolates, remains to be determined.-
dc.languageeng-
dc.publisherSage Publications, Inc. The Journal's web site is located at http://jdr.sagepub.com/-
dc.relation.ispartofJournal of Dental Research-
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.-
dc.subjectPeriodontics-
dc.subjectPlaque-
dc.subjectBacterial-
dc.subjectAntimicrobials-
dc.subjectAntimicrobial agents/inhibitors-
dc.titleProfile of metronidazole resistance among subgingival anaerobic bacteria-
dc.typeConference_Paper-
dc.identifier.emailLeung, WK: ewkleung@hkucc.hku.hk-
dc.identifier.emailWatt, RM: rmwatt@hkucc.hku.hk-
dc.identifier.emailCorbet, EF: efcorbet@hkusua.hku.hk-
dc.identifier.authorityLeung, WK=rp00019-
dc.identifier.authorityWatt, RM=rp00043-
dc.identifier.authorityCorbet, EF=rp00005-
dc.identifier.hkuros181585-
dc.identifier.volume89-
dc.identifier.issueSpec Iss C-
dc.identifier.spageAbstract no. 104-
dc.identifier.epageAbstract no. 104-
dc.publisher.placeUnited States-

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