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Article: κ-Opioid receptor stimulation increases the expression of NA+-H+ exchange gene in the heart

Titleκ-Opioid receptor stimulation increases the expression of NA+-H+ exchange gene in the heart
Authors
Issue Date1999
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 1999, v. 66 n. 4, p. 355-361 How to Cite?
Abstractκ-opioid receptor (OR) stimulation increases intracellular pH (pHi) via activating the Na+-H+ exchange (NHE). In the present study, we determined the expression of the gene of NHE 1, the predominant NHE isoform in the heart, and intracellular pH (pHi) upon κ-OR stimulation in the rat heart. We found that 1μM U50,488H (trans-3,4-dichloro-N-methyl-N-(2-(1 pyrrolidinyl)cyclohexyl)benzeneacetamide), a selective κ-OR agonist, increased the expression of the NHE 1 gene. We also found that U50,488H dose-dependently increased pHi in the heart. The effects were abolished by 1 μM nor-binaltorphimine (nor-BNI), a selective κ-OR antagonist, indicating that the events were κ-OR mediated. The effects on both NHE 1 gene expression and pHi were also abolished by 5 μM chelerythrine and 5 μM BSM (bisyndolylmaleimide), protein kinase C (PKC) inhibitors, indicating that PKC mediated the actions. In addition, the effect of U50,488H on pHi was blocked by 10 μM EIPA (ethylisopropyl amiloride), a NHE 1 inhibitor, indicating that NHE 1 also mediated the action of U50,488H. The present study provides evidence for the first time that κ-OR stimulation increased the NHE 1 gene expression in the heart via a PKC dependent pathway. κ-OR stimulation also increases pHi via PKC and NHE in the heart.
Persistent Identifierhttp://hdl.handle.net/10722/224113
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056

 

DC FieldValueLanguage
dc.contributor.authorGan, T-
dc.contributor.authorPei, J-
dc.contributor.authorChan, I-
dc.contributor.authorWong, T-
dc.date.accessioned2016-03-24T02:31:21Z-
dc.date.available2016-03-24T02:31:21Z-
dc.date.issued1999-
dc.identifier.citationLife Sciences, 1999, v. 66 n. 4, p. 355-361-
dc.identifier.issn0024-3205-
dc.identifier.urihttp://hdl.handle.net/10722/224113-
dc.description.abstractκ-opioid receptor (OR) stimulation increases intracellular pH (pHi) via activating the Na+-H+ exchange (NHE). In the present study, we determined the expression of the gene of NHE 1, the predominant NHE isoform in the heart, and intracellular pH (pHi) upon κ-OR stimulation in the rat heart. We found that 1μM U50,488H (trans-3,4-dichloro-N-methyl-N-(2-(1 pyrrolidinyl)cyclohexyl)benzeneacetamide), a selective κ-OR agonist, increased the expression of the NHE 1 gene. We also found that U50,488H dose-dependently increased pHi in the heart. The effects were abolished by 1 μM nor-binaltorphimine (nor-BNI), a selective κ-OR antagonist, indicating that the events were κ-OR mediated. The effects on both NHE 1 gene expression and pHi were also abolished by 5 μM chelerythrine and 5 μM BSM (bisyndolylmaleimide), protein kinase C (PKC) inhibitors, indicating that PKC mediated the actions. In addition, the effect of U50,488H on pHi was blocked by 10 μM EIPA (ethylisopropyl amiloride), a NHE 1 inhibitor, indicating that NHE 1 also mediated the action of U50,488H. The present study provides evidence for the first time that κ-OR stimulation increased the NHE 1 gene expression in the heart via a PKC dependent pathway. κ-OR stimulation also increases pHi via PKC and NHE in the heart.-
dc.languageeng-
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie-
dc.relation.ispartofLife Sciences-
dc.rightsPosting accepted manuscript (postprint): © <year>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.subject.meshMyocardium - metabolism-
dc.subject.meshNaltrexone - analogs & derivatives - pharmacology-
dc.subject.meshProtein Kinase C - physiology-
dc.subject.meshReceptors, Opioid, kappa - physiology-
dc.subject.meshSodium-Hydrogen Antiporter - genetics-
dc.titleκ-Opioid receptor stimulation increases the expression of NA+-H+ exchange gene in the heart-
dc.typeArticle-
dc.identifier.emailWong, T: wongtakm@hkucc.hku.hk-
dc.identifier.doi10.1016/S0024-3205(99)00597-4-
dc.identifier.pmid10665987-
dc.identifier.hkuros54134-
dc.identifier.volume66-
dc.identifier.issue4-
dc.identifier.spage355-
dc.identifier.epage361-
dc.publisher.placeUnited States-

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