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Article: AUF1-like protein binds specifically to DAS cis-acting element that regulates mouse α-fetoprotein gene expression

TitleAUF1-like protein binds specifically to DAS cis-acting element that regulates mouse α-fetoprotein gene expression
Authors
Issue Date2006
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503
Citation
Journal of Cellular Biochemistry, 2006, v. 98 n. 5, p. 1257-1270 How to Cite?
AbstractAlpha-fetoprotein (AFP) is one of the major serum proteins in the early life of mammals. We have previously identified a novel cis-acting element designated as DAS at the 5′-flanking region of the AFP gene and demonstrated that the DAS sequence can be specifically recognized by nuclear protein DAP-II in AFP-producing hepatoma cells and retinoic acid (RA)-induced AFP-producing F9 cells. In this study, we used DNA affinity chromatography to purify the DAP-II proteins from the nuclear extracts (NE) of RA-treated F9 cells. The purified DAP-II complex mainly contained five proteins, with molecular weights of 45, 42, 32, 30, and 20 kDa, respectively. The identification of these proteins was determined by MALDI-TOF mass spectrometric analysis and a database search. These proteins were found to belong to the AUF1 RNA-binding protein family. Protein (30 kDa), one of five proteins in an isolated DAP-II complex, was matched with amino acid sequence highly similar to muAUF1-3. The expression of this protein is inducible by RA, and the pattern of the protein expression is the same as DAP-II proteins in F9 cells after treatment with RA during differentiation. Our results suggest that the 30-kDa protein is a novel isoform of AUF1 family and is the main component of the DAP-II complex that binds to the DAS sequence. J. Cell. Biochem. 98: 1257–1270, 2006. © 2006 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/223740
ISSN
2015 Impact Factor: 3.446
2015 SCImago Journal Rankings: 1.520

 

DC FieldValueLanguage
dc.contributor.authorJiao, R-
dc.contributor.authorHe, QY-
dc.contributor.authorChen, H-
dc.contributor.authorHua, Z-
dc.contributor.authorJiao, Q-
dc.contributor.authorChiu, JF-
dc.date.accessioned2016-03-14T02:20:57Z-
dc.date.available2016-03-14T02:20:57Z-
dc.date.issued2006-
dc.identifier.citationJournal of Cellular Biochemistry, 2006, v. 98 n. 5, p. 1257-1270-
dc.identifier.issn0730-2312-
dc.identifier.urihttp://hdl.handle.net/10722/223740-
dc.description.abstractAlpha-fetoprotein (AFP) is one of the major serum proteins in the early life of mammals. We have previously identified a novel cis-acting element designated as DAS at the 5′-flanking region of the AFP gene and demonstrated that the DAS sequence can be specifically recognized by nuclear protein DAP-II in AFP-producing hepatoma cells and retinoic acid (RA)-induced AFP-producing F9 cells. In this study, we used DNA affinity chromatography to purify the DAP-II proteins from the nuclear extracts (NE) of RA-treated F9 cells. The purified DAP-II complex mainly contained five proteins, with molecular weights of 45, 42, 32, 30, and 20 kDa, respectively. The identification of these proteins was determined by MALDI-TOF mass spectrometric analysis and a database search. These proteins were found to belong to the AUF1 RNA-binding protein family. Protein (30 kDa), one of five proteins in an isolated DAP-II complex, was matched with amino acid sequence highly similar to muAUF1-3. The expression of this protein is inducible by RA, and the pattern of the protein expression is the same as DAP-II proteins in F9 cells after treatment with RA during differentiation. Our results suggest that the 30-kDa protein is a novel isoform of AUF1 family and is the main component of the DAP-II complex that binds to the DAS sequence. J. Cell. Biochem. 98: 1257–1270, 2006. © 2006 Wiley-Liss, Inc.-
dc.languageeng-
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503-
dc.relation.ispartofJournal of Cellular Biochemistry-
dc.rightsJournal of Cellular Biochemistry. Copyright © John Wiley & Sons, Inc.-
dc.subject.meshDNA - genetics - metabolism-
dc.subject.meshGene Expression Regulation - genetics-
dc.subject.meshHeterogeneous-Nuclear Ribonucleoprotein D - chemistry - classification - metabolism-
dc.subject.meshResponse Elements - genetics-
dc.subject.meshalpha-Fetoproteins - genetics - metabolism-
dc.titleAUF1-like protein binds specifically to DAS cis-acting element that regulates mouse α-fetoprotein gene expression-
dc.typeArticle-
dc.identifier.emailChiu, JF: jfchiu@hkucc.hku.hk-
dc.identifier.doi10.1002/jcb.20843-
dc.identifier.pmid16514630-
dc.identifier.hkuros128947-
dc.identifier.volume98-
dc.identifier.issue5-
dc.identifier.spage1257-
dc.identifier.epage1270-
dc.publisher.placeUnited States-

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