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postgraduate thesis: Discovery and comparative genomics of a novel enterovirus in camels

TitleDiscovery and comparative genomics of a novel enterovirus in camels
Authors
Issue Date2015
PublisherThe University of Hong Kong (Pokfulam, Hong Kong)
Citation
Li, T. [李彤]. (2015). Discovery and comparative genomics of a novel enterovirus in camels. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5719472
AbstractIn 2012, the Middle East Respiratory Syndrome Coronavirus was identified in the Middle East region, which contributes to severe respiratory diseases in humans. The virus was found from dromedary camels in the Middle East, an animal that the residents and travellers usually contact with. It promoted the interest to discover previously undescribed viruses in dromedary camels. Since the picornavirus is a common pathogen in animals and humans but there were few reports about picornavirus infection in dromedaries, we hypothesized that some previously unknown picornaviruses might exist in dromedaries. In the surveillance study for 215 fecal samples from dromedaries in Dubai, we discovered a novel dromedary camel enterovirus (DcEV) in four dromedary samples (one from adult camel and three from camel calves). The complete genome of DcEV was sequenced and analyzed. The genome size was 7426 bases excluding the polyadenylated tract and the open reading frame encoded 2168 amino acids. The phylogenetic result showed DcEV was clustered with members in the genus Enterovirusand was most closely related to representatives of the species Enterovirus F. DcEV and Enterovirus F shared the highes amino acid identities in the comparisons of DcEV and representatives of other species in the Enterovirus. DcEV also possessed a putative type 1 internal ribosome entry site element and gene and protein motifs similar to members of the Enterovirus. The G+C contents of DcEV were 45%, differing from those of Enterovirus F (50%) by 5%. The low Ka/Ks values of each coding gene suggested DcEV was stable evolving in dromedaries. In conclusion, DcEV belongs to the species Enterovirus in the family Picornaviridae and represents a novel species. The serological study in 172 serum samples from dromedaries in Dubai showed 52% of the dromedaries had been infected by DcEV. Further studies on the epidemiological and pathogenical features of DcEV are important to understand the novel picornavirus in this special group of animals.
DegreeMaster of Philosophy
SubjectEnteroviruses
Dept/ProgramMicrobiology
Persistent Identifierhttp://hdl.handle.net/10722/223617

 

DC FieldValueLanguage
dc.contributor.authorLi, Tong-
dc.contributor.author李彤-
dc.date.accessioned2016-03-03T23:16:50Z-
dc.date.available2016-03-03T23:16:50Z-
dc.date.issued2015-
dc.identifier.citationLi, T. [李彤]. (2015). Discovery and comparative genomics of a novel enterovirus in camels. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5719472-
dc.identifier.urihttp://hdl.handle.net/10722/223617-
dc.description.abstractIn 2012, the Middle East Respiratory Syndrome Coronavirus was identified in the Middle East region, which contributes to severe respiratory diseases in humans. The virus was found from dromedary camels in the Middle East, an animal that the residents and travellers usually contact with. It promoted the interest to discover previously undescribed viruses in dromedary camels. Since the picornavirus is a common pathogen in animals and humans but there were few reports about picornavirus infection in dromedaries, we hypothesized that some previously unknown picornaviruses might exist in dromedaries. In the surveillance study for 215 fecal samples from dromedaries in Dubai, we discovered a novel dromedary camel enterovirus (DcEV) in four dromedary samples (one from adult camel and three from camel calves). The complete genome of DcEV was sequenced and analyzed. The genome size was 7426 bases excluding the polyadenylated tract and the open reading frame encoded 2168 amino acids. The phylogenetic result showed DcEV was clustered with members in the genus Enterovirusand was most closely related to representatives of the species Enterovirus F. DcEV and Enterovirus F shared the highes amino acid identities in the comparisons of DcEV and representatives of other species in the Enterovirus. DcEV also possessed a putative type 1 internal ribosome entry site element and gene and protein motifs similar to members of the Enterovirus. The G+C contents of DcEV were 45%, differing from those of Enterovirus F (50%) by 5%. The low Ka/Ks values of each coding gene suggested DcEV was stable evolving in dromedaries. In conclusion, DcEV belongs to the species Enterovirus in the family Picornaviridae and represents a novel species. The serological study in 172 serum samples from dromedaries in Dubai showed 52% of the dromedaries had been infected by DcEV. Further studies on the epidemiological and pathogenical features of DcEV are important to understand the novel picornavirus in this special group of animals.-
dc.languageeng-
dc.publisherThe University of Hong Kong (Pokfulam, Hong Kong)-
dc.relation.ispartofHKU Theses Online (HKUTO)-
dc.rightsThe author retains all proprietary rights, (such as patent rights) and the right to use in future works.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.lcshEnteroviruses-
dc.titleDiscovery and comparative genomics of a novel enterovirus in camels-
dc.typePG_Thesis-
dc.identifier.hkulb5719472-
dc.description.thesisnameMaster of Philosophy-
dc.description.thesislevelMaster-
dc.description.thesisdisciplineMicrobiology-
dc.description.naturepublished_or_final_version-

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