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Article: Genetic variation of Alternaria alternata, an endophytic fungus isolated from Pinus tabulaeformis as determined by random amplified microsatelites (RAMS)
Title | Genetic variation of Alternaria alternata, an endophytic fungus isolated from Pinus tabulaeformis as determined by random amplified microsatelites (RAMS) |
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Authors | |
Keywords | Endophyte Genotype Pine RAMS |
Issue Date | 2004 |
Publisher | Fungal Diversity Press. The Journal's web site is located at http://www.fungaldiversity.org/fdp/jumble.php |
Citation | Fungal Diversity, 2004, v. 16, p. 53-65 How to Cite? |
Abstract | Genetic variation of 112 isolates of Alternaria alternata, an endophytic fungus isolated from
Pinus tabulaeformis, was carried out using two RAMS primers (CCA and CGA). All 1273
bands produced were clear and reproducible and ranged from 200 to 2000 base pairs. Analysis
of the two primers revealed a high level of genetic diversity (H’ = 2.79) among the 112 isolates
tested. A total of 20 markers were scored. Two markers were present in all isolates, while the
other 18 (90%) markers occurred in combinations of 105 (93.8%) different genotypes among
the 112 isolates. Genetic similarity coefficients between pairwise isolates varied from 0.3 to 1
based on an unweighted paired group method of arithmetic average (UPGMA) cluster analysis.
There was no relationship between fungal genotypes and host tissue ages, and the endophytic
fungus A. alternata appears to have the potential to evolve relatively quickly and maintain
significant genetic variation. |
Persistent Identifier | http://hdl.handle.net/10722/223487 |
ISSN | 2023 Impact Factor: 24.5 2023 SCImago Journal Rankings: 6.787 |
DC Field | Value | Language |
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dc.contributor.author | Guo, L | - |
dc.contributor.author | Xu, L | - |
dc.contributor.author | Zheng, WH | - |
dc.contributor.author | Hyde, KD | - |
dc.date.accessioned | 2016-03-01T01:44:23Z | - |
dc.date.available | 2016-03-01T01:44:23Z | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | Fungal Diversity, 2004, v. 16, p. 53-65 | - |
dc.identifier.issn | 1560-2745 | - |
dc.identifier.uri | http://hdl.handle.net/10722/223487 | - |
dc.description.abstract | Genetic variation of 112 isolates of Alternaria alternata, an endophytic fungus isolated from Pinus tabulaeformis, was carried out using two RAMS primers (CCA and CGA). All 1273 bands produced were clear and reproducible and ranged from 200 to 2000 base pairs. Analysis of the two primers revealed a high level of genetic diversity (H’ = 2.79) among the 112 isolates tested. A total of 20 markers were scored. Two markers were present in all isolates, while the other 18 (90%) markers occurred in combinations of 105 (93.8%) different genotypes among the 112 isolates. Genetic similarity coefficients between pairwise isolates varied from 0.3 to 1 based on an unweighted paired group method of arithmetic average (UPGMA) cluster analysis. There was no relationship between fungal genotypes and host tissue ages, and the endophytic fungus A. alternata appears to have the potential to evolve relatively quickly and maintain significant genetic variation. | - |
dc.language | eng | - |
dc.publisher | Fungal Diversity Press. The Journal's web site is located at http://www.fungaldiversity.org/fdp/jumble.php | - |
dc.relation.ispartof | Fungal Diversity | - |
dc.subject | Endophyte | - |
dc.subject | Genotype | - |
dc.subject | Pine | - |
dc.subject | RAMS | - |
dc.title | Genetic variation of Alternaria alternata, an endophytic fungus isolated from Pinus tabulaeformis as determined by random amplified microsatelites (RAMS) | - |
dc.type | Article | - |
dc.identifier.email | Guo, L: guold@sun.im.ac.cn | - |
dc.identifier.email | Hyde, KD: kdhyde@hkucc.hku.hk | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.scopus | eid_2-s2.0-9144237095 | - |
dc.identifier.hkuros | 93005 | - |
dc.identifier.volume | 16 | - |
dc.identifier.spage | 53 | - |
dc.identifier.epage | 65 | - |
dc.publisher.place | Hong Kong | - |
dc.identifier.issnl | 1560-2745 | - |