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Article: Bacterial expression of the shrimp molt-inhibiting hormone (MIH): antibody production, immunocytochemical study and biological assay

TitleBacterial expression of the shrimp molt-inhibiting hormone (MIH): antibody production, immunocytochemical study and biological assay
Authors
Issue Date2001
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm
Citation
Cell and Tissue Research, 2001, v. 303 n. 1, p. 129-136 How to Cite?
AbstractMolting in shrimp is controlled by the molt-inhibiting hormone (MIH) and ecdysone. MIH inhibits the synthesis of ecdysone in the Y-organ, resulting in molt suppression; it is a neuropeptide member belonging to the eyestalk CHH/MIH/GIH family. The cloning of MIH (formerly MIH-like) of the shrimp Metapenaeus ensis has been reported in a previous study. To obtain a large quantity of fusion protein for antibody production and biological assay, the cDNA encoding the shrimp MIH was inserted into the pRSET bacterial expression vector. His-tagged fusion protein was produced and purified by an Ni2+-charged affinity column. Polyclonal antibody to rMIH was subsequently obtained by immunizing rabbits with purified recombinant proteins. Results from Western blot analysis indicated that the antibody was specific. Furthermore, results from immunocytochemical analysis showed that specific cells in three different clusters of the X-organ, the sinus gland and the axonal tract of the eyestalk contain MIH. To test for the molt-inhibiting activity of rMIH, shrimp at intermolt stage were injected with rMIH and the molt cycle duration of the injected shrimp was monitored. A significant increase in molt cycle duration was recorded for the shrimp injected with the recombinant protein.
Persistent Identifierhttp://hdl.handle.net/10722/222884
ISSN
2015 Impact Factor: 2.948
2015 SCImago Journal Rankings: 1.539

 

DC FieldValueLanguage
dc.contributor.authorGu, PL-
dc.contributor.authorChu, KH-
dc.contributor.authorChan, SM-
dc.date.accessioned2016-02-05T07:45:15Z-
dc.date.available2016-02-05T07:45:15Z-
dc.date.issued2001-
dc.identifier.citationCell and Tissue Research, 2001, v. 303 n. 1, p. 129-136-
dc.identifier.issn0302-766X-
dc.identifier.urihttp://hdl.handle.net/10722/222884-
dc.description.abstractMolting in shrimp is controlled by the molt-inhibiting hormone (MIH) and ecdysone. MIH inhibits the synthesis of ecdysone in the Y-organ, resulting in molt suppression; it is a neuropeptide member belonging to the eyestalk CHH/MIH/GIH family. The cloning of MIH (formerly MIH-like) of the shrimp Metapenaeus ensis has been reported in a previous study. To obtain a large quantity of fusion protein for antibody production and biological assay, the cDNA encoding the shrimp MIH was inserted into the pRSET bacterial expression vector. His-tagged fusion protein was produced and purified by an Ni2+-charged affinity column. Polyclonal antibody to rMIH was subsequently obtained by immunizing rabbits with purified recombinant proteins. Results from Western blot analysis indicated that the antibody was specific. Furthermore, results from immunocytochemical analysis showed that specific cells in three different clusters of the X-organ, the sinus gland and the axonal tract of the eyestalk contain MIH. To test for the molt-inhibiting activity of rMIH, shrimp at intermolt stage were injected with rMIH and the molt cycle duration of the injected shrimp was monitored. A significant increase in molt cycle duration was recorded for the shrimp injected with the recombinant protein.-
dc.languageeng-
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm-
dc.relation.ispartofCell and Tissue Research-
dc.rightsThe final publication is available at Springer via http://dx.doi.org/[insert DOI]-
dc.subject.meshAntibodies - isolation & purification-
dc.subject.meshDecapoda (Crustacea) - physiology-
dc.subject.meshEye - chemistry-
dc.subject.meshInvertebrate Hormones - analysis - genetics - immunology-
dc.subject.meshNeuropeptides - analysis - genetics - immunology-
dc.titleBacterial expression of the shrimp molt-inhibiting hormone (MIH): antibody production, immunocytochemical study and biological assay-
dc.typeArticle-
dc.identifier.emailChan, SM: chansm@hkucc.hku.hk-
dc.identifier.doi10.1007/s004410000299-
dc.identifier.pmid11236000-
dc.identifier.hkuros56556-
dc.identifier.volume303-
dc.identifier.issue1-
dc.identifier.spage129-
dc.identifier.epage136-
dc.publisher.placeGermany-

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