File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Tetherin/BST-2 is essential for the formation of the intracellular virus-containing compartment in HIV-infected macrophages

TitleTetherin/BST-2 is essential for the formation of the intracellular virus-containing compartment in HIV-infected macrophages
Authors
Issue Date2012
Citation
Cell Host and Microbe, 2012, v. 12, n. 3, p. 360-372 How to Cite?
AbstractHIV-1 assembly and release occur at the plasma membrane in T lymphocytes, while intracellular sites of virus assembly or accumulation are apparent in macrophages. The host protein tetherin (BST-2) inhibits HIV release from the plasma membrane by retaining viral particles at the cell surface, but the role of tetherin at intracellular HIV assembly sites is unclear. We determined that tetherin is significantly upregulated upon macrophage infection and localizes to an intracellular virus-containing compartment (VCC). Tetherin localized at the virus-VCC membrane interface, suggesting that tetherin physically tethers virions in VCCs. Tetherin knockdown diminished and redistributed VCCs within macrophages and promoted HIV release and cell-cell transmission. The HIV Vpu protein, which downregulates tetherin from the plasma membrane, did not fully overcome tetherin-mediated restriction of particle release in macrophages. Thus, tetherin is essential for VCC formation and may account for morphologic differences in the apparent HIV assembly sites in macrophages versus T cells. © 2012 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/222652
ISSN
2015 Impact Factor: 12.552
2015 SCImago Journal Rankings: 8.283

 

DC FieldValueLanguage
dc.contributor.authorChu, Hin-
dc.contributor.authorWang, Jaang Jiun-
dc.contributor.authorQi, Mingli-
dc.contributor.authorYoon, Jeong Joong-
dc.contributor.authorChen, Xuemin-
dc.contributor.authorWen, Xiaoyun-
dc.contributor.authorHammonds, Jason-
dc.contributor.authorDing, Lingmei-
dc.contributor.authorSpearman, Paul-
dc.date.accessioned2016-01-19T03:36:45Z-
dc.date.available2016-01-19T03:36:45Z-
dc.date.issued2012-
dc.identifier.citationCell Host and Microbe, 2012, v. 12, n. 3, p. 360-372-
dc.identifier.issn1931-3128-
dc.identifier.urihttp://hdl.handle.net/10722/222652-
dc.description.abstractHIV-1 assembly and release occur at the plasma membrane in T lymphocytes, while intracellular sites of virus assembly or accumulation are apparent in macrophages. The host protein tetherin (BST-2) inhibits HIV release from the plasma membrane by retaining viral particles at the cell surface, but the role of tetherin at intracellular HIV assembly sites is unclear. We determined that tetherin is significantly upregulated upon macrophage infection and localizes to an intracellular virus-containing compartment (VCC). Tetherin localized at the virus-VCC membrane interface, suggesting that tetherin physically tethers virions in VCCs. Tetherin knockdown diminished and redistributed VCCs within macrophages and promoted HIV release and cell-cell transmission. The HIV Vpu protein, which downregulates tetherin from the plasma membrane, did not fully overcome tetherin-mediated restriction of particle release in macrophages. Thus, tetherin is essential for VCC formation and may account for morphologic differences in the apparent HIV assembly sites in macrophages versus T cells. © 2012 Elsevier Inc.-
dc.languageeng-
dc.relation.ispartofCell Host and Microbe-
dc.titleTetherin/BST-2 is essential for the formation of the intracellular virus-containing compartment in HIV-infected macrophages-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.chom.2012.07.011-
dc.identifier.pmid22980332-
dc.identifier.scopuseid_2-s2.0-84866372498-
dc.identifier.volume12-
dc.identifier.issue3-
dc.identifier.spage360-
dc.identifier.epage372-
dc.identifier.eissn1934-6069-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats