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Article: Full-Genome Deep Sequencing and Phylogenetic Analysis of Novel Human Betacoronavirus

TitleFull-Genome Deep Sequencing and Phylogenetic Analysis of Novel Human Betacoronavirus
Authors
KeywordsDisease transmission
Infectious
Coronavirus infections
Evolution
Molecular
Issue Date2013
PublisherUS Department of Health and Human Services, Centers for Disease Control and Prevention. The Journal's web site is located at http://www.cdc.gov/ncidod/EID/index.htm
Citation
Emerging Infectious Diseases, 2013, v. 19 n. 5, p. 736-742 How to Cite?
AbstractA novel betacoronavirus associated with lethal respiratory and renal complications was recently identified in patients from several countries in the Middle East. We report the deep genome sequencing of the virus directly from a patient’s sputum sample. Our high-throughput sequencing yielded a substantial depth of genome sequence assembly and showed the minority viral variants in the specimen. Detailed phylogenetic analysis of the virus genome (England/Qatar/2012) revealed its close relationship to European bat coronaviruses circulating among the bat species of the Vespertilionidae family. Molecular clock analysis showed that the 2 human infections of this betacoronavirus in June 2012 (EMC/2012) and September 2012 (England/Qatar/2012) share a common virus ancestor most likely considerably before early 2012, suggesting the human diversity is the result of multiple zoonotic events.
Persistent Identifierhttp://hdl.handle.net/10722/221824
ISSN
2015 Impact Factor: 6.994
2015 SCImago Journal Rankings: 3.023
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCotten, M-
dc.contributor.authorLam, TY-
dc.contributor.authorWatson, SJ-
dc.contributor.authorPalser, AL-
dc.contributor.authorPetrova, V-
dc.contributor.authorGrant, P-
dc.contributor.authorPybus, OG-
dc.contributor.authorRambaut, A-
dc.contributor.authorGuan, Y-
dc.contributor.authorPillay, D-
dc.contributor.authorKellam, P-
dc.contributor.authorNastouli, E-
dc.date.accessioned2015-12-10T03:15:33Z-
dc.date.available2015-12-10T03:15:33Z-
dc.date.issued2013-
dc.identifier.citationEmerging Infectious Diseases, 2013, v. 19 n. 5, p. 736-742-
dc.identifier.issn1080-6040-
dc.identifier.urihttp://hdl.handle.net/10722/221824-
dc.description.abstractA novel betacoronavirus associated with lethal respiratory and renal complications was recently identified in patients from several countries in the Middle East. We report the deep genome sequencing of the virus directly from a patient’s sputum sample. Our high-throughput sequencing yielded a substantial depth of genome sequence assembly and showed the minority viral variants in the specimen. Detailed phylogenetic analysis of the virus genome (England/Qatar/2012) revealed its close relationship to European bat coronaviruses circulating among the bat species of the Vespertilionidae family. Molecular clock analysis showed that the 2 human infections of this betacoronavirus in June 2012 (EMC/2012) and September 2012 (England/Qatar/2012) share a common virus ancestor most likely considerably before early 2012, suggesting the human diversity is the result of multiple zoonotic events.-
dc.languageeng-
dc.publisherUS Department of Health and Human Services, Centers for Disease Control and Prevention. The Journal's web site is located at http://www.cdc.gov/ncidod/EID/index.htm-
dc.relation.ispartofEmerging Infectious Diseases-
dc.subjectDisease transmission-
dc.subjectInfectious-
dc.subjectCoronavirus infections-
dc.subjectEvolution-
dc.subjectMolecular-
dc.titleFull-Genome Deep Sequencing and Phylogenetic Analysis of Novel Human Betacoronavirus-
dc.typeArticle-
dc.identifier.emailLam, TY: ttylam@hku.hk-
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hk-
dc.identifier.authorityLam, TY=rp01733-
dc.identifier.authorityGuan, Y=rp00397-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.3201/eid1905.130057-
dc.identifier.pmcidPMC3647518-
dc.identifier.volume19-
dc.identifier.issue5-
dc.identifier.spage736-
dc.identifier.epage742-
dc.identifier.isiWOS:000328173400005-
dc.publisher.placeUnited States-

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