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postgraduate thesis: Molecular detection of Streptobacillus hongkongensis in human nasopharynx in Hong Kong

TitleMolecular detection of Streptobacillus hongkongensis in human nasopharynx in Hong Kong
Authors
Issue Date2015
PublisherThe University of Hong Kong (Pokfulam, Hong Kong)
Citation
Ho, M. [何敏玲]. (2015). Molecular detection of Streptobacillus hongkongensis in human nasopharynx in Hong Kong. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5626195
AbstractStreptobacillus hongkongensis is a novel member of the genus Streptobacillus. It is a fastidious and pleomorphic Gram-negative bacillus which has been isolated recently in two streptobacillary like infections reported in Hong Kong. Bacterial strain 〖 HKU33〗^T was firstly isolated from the peritonsillar abscess while strain HKU34 was isolated from the joint fluid of another individual. Regarding the phylogenetic study of the complete 16S rRNA gene, partial groEL, gyrB and recA gene sequences, 〖 HKU33〗^T and HKU34 relate most closely to Streptobacillus moniliformis, which is one of the etiological agent of widespread rat bite fever. Nevertheless, there is no information on the prevalence of S. hongkongensis and its role in human. As the nasopharynx of rats is one of the dominant natural reservoir of S. moniliformis, a bacterium highly related species to S. hongkongensis, whether S. hongkongensis also naturally resides in the nasopharynx of rats or other animals, such as human, is of research interest. In this study, human nasopharyngeal swabs were collected for bacterial culture. Total bacterial DNA were then collected and the presence of S. hongkongensis genomic DNA in the samples were screened by PCR-amplification, using the 16 rRNA gene as the DNA marker and specific primers. The partial bacterial 16S rRNA gene sequences obtained were compared with those of other bacteria in the GenBank database. Out of 277 samples, 45 yielded DNA fragments which shared > 97% partial 16S rRNA gene sequence homology with S. hongkongensis〖 HKU33〗^T, indicating that the prevalence of S. hongkongensis in human nasopharynx is 16.2%
DegreeMaster of Medical Sciences
SubjectStreptobacillus
Dept/ProgramMicrobiology
Persistent Identifierhttp://hdl.handle.net/10722/221467

 

DC FieldValueLanguage
dc.contributor.authorHo, Man-ling-
dc.contributor.author何敏玲-
dc.date.accessioned2015-11-26T23:34:47Z-
dc.date.available2015-11-26T23:34:47Z-
dc.date.issued2015-
dc.identifier.citationHo, M. [何敏玲]. (2015). Molecular detection of Streptobacillus hongkongensis in human nasopharynx in Hong Kong. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5626195-
dc.identifier.urihttp://hdl.handle.net/10722/221467-
dc.description.abstractStreptobacillus hongkongensis is a novel member of the genus Streptobacillus. It is a fastidious and pleomorphic Gram-negative bacillus which has been isolated recently in two streptobacillary like infections reported in Hong Kong. Bacterial strain 〖 HKU33〗^T was firstly isolated from the peritonsillar abscess while strain HKU34 was isolated from the joint fluid of another individual. Regarding the phylogenetic study of the complete 16S rRNA gene, partial groEL, gyrB and recA gene sequences, 〖 HKU33〗^T and HKU34 relate most closely to Streptobacillus moniliformis, which is one of the etiological agent of widespread rat bite fever. Nevertheless, there is no information on the prevalence of S. hongkongensis and its role in human. As the nasopharynx of rats is one of the dominant natural reservoir of S. moniliformis, a bacterium highly related species to S. hongkongensis, whether S. hongkongensis also naturally resides in the nasopharynx of rats or other animals, such as human, is of research interest. In this study, human nasopharyngeal swabs were collected for bacterial culture. Total bacterial DNA were then collected and the presence of S. hongkongensis genomic DNA in the samples were screened by PCR-amplification, using the 16 rRNA gene as the DNA marker and specific primers. The partial bacterial 16S rRNA gene sequences obtained were compared with those of other bacteria in the GenBank database. Out of 277 samples, 45 yielded DNA fragments which shared > 97% partial 16S rRNA gene sequence homology with S. hongkongensis〖 HKU33〗^T, indicating that the prevalence of S. hongkongensis in human nasopharynx is 16.2%-
dc.languageeng-
dc.publisherThe University of Hong Kong (Pokfulam, Hong Kong)-
dc.relation.ispartofHKU Theses Online (HKUTO)-
dc.rightsThe author retains all proprietary rights, (such as patent rights) and the right to use in future works.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.lcshStreptobacillus-
dc.titleMolecular detection of Streptobacillus hongkongensis in human nasopharynx in Hong Kong-
dc.typePG_Thesis-
dc.identifier.hkulb5626195-
dc.description.thesisnameMaster of Medical Sciences-
dc.description.thesislevelMaster-
dc.description.thesisdisciplineMicrobiology-
dc.description.naturepublished_or_final_version-

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