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Article: Affinity purification in target identification: the specificity challenge

TitleAffinity purification in target identification: the specificity challenge
Authors
KeywordsTarget identification
Affinity purification
Small molecule
Phenotypic screening
Protein–ligand interaction
Issue Date2015
PublisherPharmaceutical Society of Korea. The Journal's web site is located at http://www.springer.com/biomed/pharmaceutical+science/journal/12272
Citation
Archives of Pharmacal Research, 2015, v. 38 n. 9, p. 1661-1685 How to Cite?
AbstractSince phenotype-based screening directly evaluates capability of small molecules for modulating biology in actual biological systems, it has become an important discover modality in modern pharmaceutical sciences. However, in order to fully elucidate the molecular mechanism underlying the bioactivity of small molecules, identification of their biological targets is an indispensable step. Among the many target identification strategies developed during the past several decades, affinity purification remains to be one of the most important and powerful approaches, as it can directly reveal the physical interactions between small molecules and their biomolecular targets. However, due to the complexity of the proteome and the diversity of small molecule-protein interactions, affinity purification faces the specificity challenge: how to identify the true specific targets from the non-specific background? Focusing on this challenge, in this review, we briefly introduce the history and background of affinity purification, and then we discussed the major technological developments aiming to address this challenge. We have summarized these approaches in two categories: noise reduction and comparative distinction. This review also highlights the importance of choosing an integrated approach combining multiple methods to achieving success in target identification. © 2015 The Pharmaceutical Society of Korea.
Persistent Identifierhttp://hdl.handle.net/10722/221111
ISSN
2015 Impact Factor: 2.49
2015 SCImago Journal Rankings: 0.666
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZheng, W-
dc.contributor.authorLi, G-
dc.contributor.authorLi, X-
dc.date.accessioned2015-10-27T06:58:55Z-
dc.date.available2015-10-27T06:58:55Z-
dc.date.issued2015-
dc.identifier.citationArchives of Pharmacal Research, 2015, v. 38 n. 9, p. 1661-1685-
dc.identifier.issn0253-6269-
dc.identifier.urihttp://hdl.handle.net/10722/221111-
dc.description.abstractSince phenotype-based screening directly evaluates capability of small molecules for modulating biology in actual biological systems, it has become an important discover modality in modern pharmaceutical sciences. However, in order to fully elucidate the molecular mechanism underlying the bioactivity of small molecules, identification of their biological targets is an indispensable step. Among the many target identification strategies developed during the past several decades, affinity purification remains to be one of the most important and powerful approaches, as it can directly reveal the physical interactions between small molecules and their biomolecular targets. However, due to the complexity of the proteome and the diversity of small molecule-protein interactions, affinity purification faces the specificity challenge: how to identify the true specific targets from the non-specific background? Focusing on this challenge, in this review, we briefly introduce the history and background of affinity purification, and then we discussed the major technological developments aiming to address this challenge. We have summarized these approaches in two categories: noise reduction and comparative distinction. This review also highlights the importance of choosing an integrated approach combining multiple methods to achieving success in target identification. © 2015 The Pharmaceutical Society of Korea.-
dc.languageeng-
dc.publisherPharmaceutical Society of Korea. The Journal's web site is located at http://www.springer.com/biomed/pharmaceutical+science/journal/12272-
dc.relation.ispartofArchives of Pharmacal Research-
dc.subjectTarget identification-
dc.subjectAffinity purification-
dc.subjectSmall molecule-
dc.subjectPhenotypic screening-
dc.subjectProtein–ligand interaction-
dc.titleAffinity purification in target identification: the specificity challenge-
dc.typeArticle-
dc.identifier.emailLi, X: xiaoyuli@hku.hk-
dc.identifier.authorityLi, X=rp02080-
dc.identifier.doi10.1007/s12272-015-0635-2-
dc.identifier.pmid26248768-
dc.identifier.scopuseid_2-s2.0-84942193082-
dc.identifier.volume38-
dc.identifier.issue9-
dc.identifier.spage1661-
dc.identifier.epage1685-
dc.identifier.isiWOS:000361445000011-
dc.publisher.placeKorea, Republic of-

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