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Article: Origin, Course, And Endings Of Abnormal Enteric Nerve Fibres In Hirschsprung's Disease Defined Of Whole-Mount Immunohistochemistry

TitleOrigin, Course, And Endings Of Abnormal Enteric Nerve Fibres In Hirschsprung's Disease Defined Of Whole-Mount Immunohistochemistry
Authors
Issue Date1990
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jpedsurg
Citation
Journal Of Pediatric Surgery, 1990, v. 25 n. 4, p. 457-461 How to Cite?
AbstractAccurate Delineation Of The Intramural Pathway Of Abnormal Enteric Nerve Fibres In Hirschsprung's Disease Has Previously Proved Impossible Because The Neural Network Is Invariably Transected In Conventional Histological Sections. With The Technique Of Wholemount Immunohistochemistry (Wi), The Bowel Segment Is Converted Into A Rectangular Sheet And The Serosa, Long Muscle (Lm), Circular Muscle (Cm), Submucosa, And Mucosa Are Separated Into Layers To Allow Each Nerve Plexus To Be Examined Intact And Neural Pathways Traced. The Entire Resected Bowel Specimens Of Nine Hd Infants And Five Infants Serving As Controls Were Investigated, Using Neuron-Specific Enolase And Vasoactive Intestinal Peptide (Vip) For Wi. The Major New Findings Are (1) More Vip Fibres Were Observed In Aganglionic Bowel With Wi Than With Conventional Sections; (2) Thick Nerve Trunks In Aganglionic Bowel Do Not Descend From Intrinsic Neurons Of Oligoganglionic Bowel As Previously Suggested, But Have An Extrinsic Origin, Accompanying Blood Vessels As Small Nerves Initially, Expanding Subsequently, And Ending Blindly In Submucosa; (3) Cm Nerve Fibres Follow Muscle Fibres Concentrically For Long Distances In Aganglionic Bowel; And (4) Lm Nerve Fibres Meander In Spirals In Aganglionic Bowel Instead Of Running Straight. This Study Shows That (1) Wi Is Highly Sensitive; (2) Nerve Fibres In Aganglionic Bowel Have An Extrinsic Origin; And (3) Innervation Abnormalities In Hirschsprung's Disease Are Not Only Quantitative But Qualitative.
Persistent Identifierhttp://hdl.handle.net/10722/220806
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.802
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTam, PKHen_US
dc.contributor.authorBoyd, GPen_US
dc.date.accessioned2015-10-19T04:01:40Z-
dc.date.available2015-10-19T04:01:40Z-
dc.date.issued1990-
dc.identifier.citationJournal Of Pediatric Surgery, 1990, v. 25 n. 4, p. 457-461en_US
dc.identifier.issn0022-3468-
dc.identifier.urihttp://hdl.handle.net/10722/220806-
dc.description.abstractAccurate Delineation Of The Intramural Pathway Of Abnormal Enteric Nerve Fibres In Hirschsprung's Disease Has Previously Proved Impossible Because The Neural Network Is Invariably Transected In Conventional Histological Sections. With The Technique Of Wholemount Immunohistochemistry (Wi), The Bowel Segment Is Converted Into A Rectangular Sheet And The Serosa, Long Muscle (Lm), Circular Muscle (Cm), Submucosa, And Mucosa Are Separated Into Layers To Allow Each Nerve Plexus To Be Examined Intact And Neural Pathways Traced. The Entire Resected Bowel Specimens Of Nine Hd Infants And Five Infants Serving As Controls Were Investigated, Using Neuron-Specific Enolase And Vasoactive Intestinal Peptide (Vip) For Wi. The Major New Findings Are (1) More Vip Fibres Were Observed In Aganglionic Bowel With Wi Than With Conventional Sections; (2) Thick Nerve Trunks In Aganglionic Bowel Do Not Descend From Intrinsic Neurons Of Oligoganglionic Bowel As Previously Suggested, But Have An Extrinsic Origin, Accompanying Blood Vessels As Small Nerves Initially, Expanding Subsequently, And Ending Blindly In Submucosa; (3) Cm Nerve Fibres Follow Muscle Fibres Concentrically For Long Distances In Aganglionic Bowel; And (4) Lm Nerve Fibres Meander In Spirals In Aganglionic Bowel Instead Of Running Straight. This Study Shows That (1) Wi Is Highly Sensitive; (2) Nerve Fibres In Aganglionic Bowel Have An Extrinsic Origin; And (3) Innervation Abnormalities In Hirschsprung's Disease Are Not Only Quantitative But Qualitative.en_US
dc.languageengen_US
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jpedsurgen_US
dc.relation.ispartofJournal Of Pediatric Surgeryen_US
dc.titleOrigin, Course, And Endings Of Abnormal Enteric Nerve Fibres In Hirschsprung's Disease Defined Of Whole-Mount Immunohistochemistryen_US
dc.typeArticleen_US
dc.identifier.emailTam, PKH:paultam@hkucc.hku.hk-
dc.identifier.authorityTam, PKH=rp00060-
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0022-3468(90)90394-O-
dc.identifier.pmid2329466-
dc.identifier.scopuseid_2-s2.0-0025305714-
dc.identifier.volume25-
dc.identifier.issue4-
dc.identifier.spage457-
dc.identifier.epage461-
dc.identifier.isiWOS:A1990CX79100021-

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