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Article: Abnormalities Of Nitric-Oxide-Producing Neurons In Hirschsprung's Disease: Morphology And Implications

TitleAbnormalities Of Nitric-Oxide-Producing Neurons In Hirschsprung's Disease: Morphology And Implications
Authors
Issue Date1994
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jpedsurg
Citation
Journal Of Pediatric Surgery, 1994, v. 29 n. 2, p. 294-300 How to Cite?
AbstractNitric Oxide (No) Is A Recently Discovered Neurotransmitter That Is Thought To Mediate Relaxation Of Gut Smooth Muscle During Peristalsis. To Assess Its Role In The Pathophysiology Of Hirschsprung's Disease, The Authors Examined The Distribution Of Neurons That Produce No In Specimens From Seven Infants With This Condition. Immunohistochemical Analysis Of Cryostat Sections For Nitric Oxide Synthase (Nos) Immunoreactivity (Nos Catalyzes The Production Of No) Showed That Nos Is Localized In A Substantial Subpopulation Of Enteric Neurons In Both The Myenteric And Submucosal Plexuses In The Ganglionated Gut, But It Was Completely Absent In Aganglionic Bowel. Nos Immunoreactivity Specifically Colocalizes In Neurons That Also Contain Nadph- Diaphorase Activity. This Finding Enabled The Distribution Of No-Producing Neurons To Be Determined Using Whole-Mount Histochemistry, A Technique That Allows The Enteric Neural Network To Be Examined Intact. In Normal Bowel, No- Producing Neurons Are Arranged In Star-Shaped Myenteric And Submucosal Ganglia, Which Are Joined To One Another By Nerve Fibers To Form A Meshwork Of Variable Geometry. Individual Neurons Exhibit Dogiel Type 1 Morphology. Axonal Processes Leave The Myenteric Plexuses And Lie Parallel To Muscle Bundles In Both Muscle Layers Of The Gut. In The Transition Zone, Ganglia Are Initially Present, But Their Orientation Changes So That Both They And The Internodal Strands That Connect Them Are Aligned Linearly Along The Craniocaudal Axis Of The Gut Tube. More Distal Still, Ganglia And Then All Nos Activity Disappear Completely. These Results Suggest That In Hirschsprung's Disease, The Failure Of Aganglionic Bowel To Relax During Peristalsis Might Be Caused By The Absence Of No-Producing Neurons.
Persistent Identifierhttp://hdl.handle.net/10722/220788
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.802
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorO'kelly, TJen_US
dc.contributor.authorDavies, JRen_US
dc.contributor.authorTam, PKHen_US
dc.contributor.authorBrading, AFen_US
dc.contributor.authorMortensen, NJMCen_US
dc.date.accessioned2015-10-19T03:43:04Z-
dc.date.available2015-10-19T03:43:04Z-
dc.date.issued1994-
dc.identifier.citationJournal Of Pediatric Surgery, 1994, v. 29 n. 2, p. 294-300en_US
dc.identifier.issn0022-3468-
dc.identifier.urihttp://hdl.handle.net/10722/220788-
dc.description.abstractNitric Oxide (No) Is A Recently Discovered Neurotransmitter That Is Thought To Mediate Relaxation Of Gut Smooth Muscle During Peristalsis. To Assess Its Role In The Pathophysiology Of Hirschsprung's Disease, The Authors Examined The Distribution Of Neurons That Produce No In Specimens From Seven Infants With This Condition. Immunohistochemical Analysis Of Cryostat Sections For Nitric Oxide Synthase (Nos) Immunoreactivity (Nos Catalyzes The Production Of No) Showed That Nos Is Localized In A Substantial Subpopulation Of Enteric Neurons In Both The Myenteric And Submucosal Plexuses In The Ganglionated Gut, But It Was Completely Absent In Aganglionic Bowel. Nos Immunoreactivity Specifically Colocalizes In Neurons That Also Contain Nadph- Diaphorase Activity. This Finding Enabled The Distribution Of No-Producing Neurons To Be Determined Using Whole-Mount Histochemistry, A Technique That Allows The Enteric Neural Network To Be Examined Intact. In Normal Bowel, No- Producing Neurons Are Arranged In Star-Shaped Myenteric And Submucosal Ganglia, Which Are Joined To One Another By Nerve Fibers To Form A Meshwork Of Variable Geometry. Individual Neurons Exhibit Dogiel Type 1 Morphology. Axonal Processes Leave The Myenteric Plexuses And Lie Parallel To Muscle Bundles In Both Muscle Layers Of The Gut. In The Transition Zone, Ganglia Are Initially Present, But Their Orientation Changes So That Both They And The Internodal Strands That Connect Them Are Aligned Linearly Along The Craniocaudal Axis Of The Gut Tube. More Distal Still, Ganglia And Then All Nos Activity Disappear Completely. These Results Suggest That In Hirschsprung's Disease, The Failure Of Aganglionic Bowel To Relax During Peristalsis Might Be Caused By The Absence Of No-Producing Neurons.en_US
dc.languageengen_US
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jpedsurgen_US
dc.relation.ispartofJournal Of Pediatric Surgeryen_US
dc.titleAbnormalities Of Nitric-Oxide-Producing Neurons In Hirschsprung's Disease: Morphology And Implicationsen_US
dc.typeArticleen_US
dc.identifier.emailTam, PKH:paultam@hkucc.hku.hk-
dc.identifier.authorityTam, PKH=rp00060-
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0022-3468(94)90335-2-
dc.identifier.pmid7513759-
dc.identifier.scopuseid_2-s2.0-0028011021-
dc.identifier.volume29-
dc.identifier.issue2-
dc.identifier.spage294-
dc.identifier.epage300-
dc.identifier.isiWOS:A1994MX66700030-

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