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- Publisher Website: 10.1016/j.jpedsurg.2012.08.026
- Scopus: eid_2-s2.0-84875184851
- PMID: 23480922
- WOS: WOS:000316470100033
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Article: Potential use of skin-derived precursors (SKPs) in establishing a cell-based treatment model for Hirschsprung's disease
Title | Potential use of skin-derived precursors (SKPs) in establishing a cell-based treatment model for Hirschsprung's disease |
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Authors | |
Keywords | Hirschsprung's (HSCR) disease Stem cell Skin-derived precursor cells (SKPs) Neural differentiation Neural crest Cell-based therapy |
Issue Date | 2013 |
Citation | Journal of Pediatric Surgery, 2013, v. 48, n. 3, p. 619-628 How to Cite? |
Abstract | Background: Hirschsprung's (HSCR) disease is characterized by absence of ganglia in the distant bowel. Skin-derived precursor cells (SKPs) are somatic stem cells located in the bulge of hair follicles with high neural plasticity. In this study, we elucidated the therapeutic potential of SKPs for replenishing absent ganglia in HSCR bowel. Methods: SKPs were isolated from mouse or human skin and cultured in neural differentiation medium to generate various types of neural cells. Expression of stem cell and neural differentiation markers were monitored by reverse-transcription polymerase chain reaction and immunocytochemistry, respectively. Engraftment and differentiation potentials of SKPs were further assessed using ex vivo gut culture with Retk/k aganglionic gut. Results: Expression studies revealed that SKPs express a panel of neural crest markers and three key stemness factors (Klf4, c-Myc and Sox2), which may account for the multipotency of these cells. Subsequent differentiation assays directly demonstrated that both mouse and human SKPs retain high differentiation capacities to form enteric neurons, and glia. Importantly, with ex vivo gut explants assay, we further showed that SKPs colonize and differentiate in the Retk/k aganglionic hindgut explants. Conclusion: Our data suggest that SKPs may represent an alternative source of stem cells for the study of cell-based therapy for HSCR. © 2013 Elsevier Inc. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/220747 |
ISSN | 2023 Impact Factor: 2.4 2023 SCImago Journal Rankings: 0.949 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Kwok, Carmen Ka Man | - |
dc.contributor.author | Tam, Paul Kwong Hang | - |
dc.contributor.author | Ngan, Elly Sau Wai | - |
dc.date.accessioned | 2015-10-16T06:50:26Z | - |
dc.date.available | 2015-10-16T06:50:26Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Journal of Pediatric Surgery, 2013, v. 48, n. 3, p. 619-628 | - |
dc.identifier.issn | 0022-3468 | - |
dc.identifier.uri | http://hdl.handle.net/10722/220747 | - |
dc.description.abstract | Background: Hirschsprung's (HSCR) disease is characterized by absence of ganglia in the distant bowel. Skin-derived precursor cells (SKPs) are somatic stem cells located in the bulge of hair follicles with high neural plasticity. In this study, we elucidated the therapeutic potential of SKPs for replenishing absent ganglia in HSCR bowel. Methods: SKPs were isolated from mouse or human skin and cultured in neural differentiation medium to generate various types of neural cells. Expression of stem cell and neural differentiation markers were monitored by reverse-transcription polymerase chain reaction and immunocytochemistry, respectively. Engraftment and differentiation potentials of SKPs were further assessed using ex vivo gut culture with Retk/k aganglionic gut. Results: Expression studies revealed that SKPs express a panel of neural crest markers and three key stemness factors (Klf4, c-Myc and Sox2), which may account for the multipotency of these cells. Subsequent differentiation assays directly demonstrated that both mouse and human SKPs retain high differentiation capacities to form enteric neurons, and glia. Importantly, with ex vivo gut explants assay, we further showed that SKPs colonize and differentiate in the Retk/k aganglionic hindgut explants. Conclusion: Our data suggest that SKPs may represent an alternative source of stem cells for the study of cell-based therapy for HSCR. © 2013 Elsevier Inc. All rights reserved. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Pediatric Surgery | - |
dc.subject | Hirschsprung's (HSCR) disease | - |
dc.subject | Stem cell | - |
dc.subject | Skin-derived precursor cells (SKPs) | - |
dc.subject | Neural differentiation | - |
dc.subject | Neural crest | - |
dc.subject | Cell-based therapy | - |
dc.title | Potential use of skin-derived precursors (SKPs) in establishing a cell-based treatment model for Hirschsprung's disease | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.jpedsurg.2012.08.026 | - |
dc.identifier.pmid | 23480922 | - |
dc.identifier.scopus | eid_2-s2.0-84875184851 | - |
dc.identifier.hkuros | 214472 | - |
dc.identifier.volume | 48 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 619 | - |
dc.identifier.epage | 628 | - |
dc.identifier.eissn | 1531-5037 | - |
dc.identifier.isi | WOS:000316470100033 | - |
dc.identifier.issnl | 0022-3468 | - |