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Article: A Comprehensive Analysis of Replicative Lifespan in 4,698 Single-Gene Deletion Strains Uncovers Conserved Mechanisms of Aging

TitleA Comprehensive Analysis of Replicative Lifespan in 4,698 Single-Gene Deletion Strains Uncovers Conserved Mechanisms of Aging
Authors
Issue Date2015
Citation
Cell Metabolism, 2015, v. 22, p. 1-12 How to Cite?
AbstractMany genes that affect replicative lifespan (RLS) in the budding yeast Saccharomyces cerevisiae also affect aging in other organisms such as C. elegans and M. musculus. We performed a systematic analysis of yeast RLS in a set of 4,698 viable single-gene deletion strains. Multiple functional gene clusters were identified, and full genome-to-genome comparison demonstrated a significant conservation in longevity pathways between yeast and C. elegans. Among the mechanisms of aging identified, deletion of tRNA exporter LOS1 robustly extended lifespan. Dietary restriction (DR) and inhibition of mechanistic Target of Rapamycin (mTOR) exclude Los1 from the nucleus in a Rad53-dependent manner. Moreover, lifespan extension from deletion of LOS1 is nonadditive with DR or mTOR inhibition, and results in Gcn4 transcription factor activation. Thus, the DNA damage response and mTOR converge on Los1-mediated nuclear tRNA export to regulate Gcn4 activity and aging.
Persistent Identifierhttp://hdl.handle.net/10722/220090

 

DC FieldValueLanguage
dc.contributor.authorMcCormick, M-
dc.contributor.authorDelaney, J-
dc.contributor.authorTsuchiya, M-
dc.contributor.authorTsuchiyama, S-
dc.contributor.authorShemorry, A-
dc.contributor.authorSim, S-
dc.contributor.authorChau, A-
dc.contributor.authorAhmed, U-
dc.contributor.authorCARR, D-
dc.contributor.authorMurakami, C-
dc.contributor.authorSchleit, J-
dc.contributor.authorSutphin, G-
dc.contributor.authorWasko, B-
dc.contributor.authorBennett, C-
dc.contributor.authorWang, A-
dc.contributor.authorOlsen, B-
dc.contributor.authorBeyer, R-
dc.contributor.authorBammler, T-
dc.contributor.authorPrunkard, D-
dc.contributor.authorJohnson, S-
dc.contributor.authorPennypacker, J-
dc.contributor.authorAn, E-
dc.contributor.authorAnies, A-
dc.contributor.authorCastanza, A-
dc.contributor.authorChoi, E-
dc.contributor.authorDang, N-
dc.contributor.authorEnerio, S-
dc.contributor.authorFletcher, M-
dc.contributor.authorFox, L-
dc.contributor.authorGoswami, S-
dc.contributor.authorHiggins, S-
dc.contributor.authorHolmberg, M-
dc.contributor.authorHu, D-
dc.contributor.authorHui, J-
dc.contributor.authorJelic, M-
dc.contributor.authorJeong, K-
dc.contributor.authorJohnston, E-
dc.contributor.authorKerr, E-
dc.contributor.authorKim, J-
dc.contributor.authorKim, D-
dc.contributor.authorKirkland, K-
dc.contributor.authorKlum, S-
dc.contributor.authorKotireddy, S-
dc.contributor.authorLiao, E-
dc.contributor.authorLim, M-
dc.contributor.authorLin, M-
dc.contributor.authorLo, W-
dc.contributor.authorLockshon, D-
dc.contributor.authorMiller, H-
dc.contributor.authorMoller, R-
dc.contributor.authorMuller, B-
dc.contributor.authorOakes, J-
dc.contributor.authorPak, D-
dc.contributor.authorPeng, Z-
dc.contributor.authorPham, K-
dc.contributor.authorPollard, T-
dc.contributor.authorPradeep, P-
dc.contributor.authorPruett, D-
dc.contributor.authorRai, D-
dc.contributor.authorRobison, B-
dc.contributor.authorRodriguez, A-
dc.contributor.authorRos, B-
dc.contributor.authorSage, M-
dc.contributor.authorSingh, M-
dc.contributor.authorSmith, E-
dc.contributor.authorSnead, K-
dc.contributor.authorSolanky, A-
dc.contributor.authorSpector, B-
dc.contributor.authorSteffen, K-
dc.contributor.authorTchao, B-
dc.contributor.authorTing, M-
dc.contributor.authorVander Wender, H-
dc.contributor.authorWang, D-
dc.contributor.authorWelton, K-
dc.contributor.authorWestman, E-
dc.contributor.authorBrem, R-
dc.contributor.authorLiu, X-
dc.contributor.authorSuh, Y-
dc.contributor.authorZhou, Z-
dc.contributor.authorKaeberlain, M-
dc.contributor.authorKennedy, B-
dc.date.accessioned2015-10-16T06:28:47Z-
dc.date.available2015-10-16T06:28:47Z-
dc.date.issued2015-
dc.identifier.citationCell Metabolism, 2015, v. 22, p. 1-12-
dc.identifier.urihttp://hdl.handle.net/10722/220090-
dc.description.abstractMany genes that affect replicative lifespan (RLS) in the budding yeast Saccharomyces cerevisiae also affect aging in other organisms such as C. elegans and M. musculus. We performed a systematic analysis of yeast RLS in a set of 4,698 viable single-gene deletion strains. Multiple functional gene clusters were identified, and full genome-to-genome comparison demonstrated a significant conservation in longevity pathways between yeast and C. elegans. Among the mechanisms of aging identified, deletion of tRNA exporter LOS1 robustly extended lifespan. Dietary restriction (DR) and inhibition of mechanistic Target of Rapamycin (mTOR) exclude Los1 from the nucleus in a Rad53-dependent manner. Moreover, lifespan extension from deletion of LOS1 is nonadditive with DR or mTOR inhibition, and results in Gcn4 transcription factor activation. Thus, the DNA damage response and mTOR converge on Los1-mediated nuclear tRNA export to regulate Gcn4 activity and aging.-
dc.languageeng-
dc.relation.ispartofCell Metabolism-
dc.titleA Comprehensive Analysis of Replicative Lifespan in 4,698 Single-Gene Deletion Strains Uncovers Conserved Mechanisms of Aging-
dc.typeArticle-
dc.identifier.emailZhou, Z: zhongjun@hku.hk-
dc.identifier.authorityZhou, Z=rp00503-
dc.identifier.doi10.1016/j.cmet.2015.09.008-
dc.identifier.hkuros256137-
dc.identifier.volume22-
dc.identifier.spage1-
dc.identifier.epage12-

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