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Article: Neuropilin 1 is an entry factor that promotes EBV infection of nasopharyngeal epithelial cells

TitleNeuropilin 1 is an entry factor that promotes EBV infection of nasopharyngeal epithelial cells
Authors
Issue Date2015
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/ncomms/index.html
Citation
Nature Communications, 2015, v. 6, p. Article no. 6240 How to Cite?
AbstractEpstein-Barr virus (EBV) is implicated as an aetiological factor in B lymphomas and nasopharyngeal carcinoma. The mechanisms of cell-free EBV infection of nasopharyngeal epithelial cells remain elusive. EBV glycoprotein B (gB) is the critical fusion protein for infection of both B and epithelial cells, and determines EBV susceptibility of non-B cells. Here we show that neuropilin 1 (NRP1) directly interacts with EBV gB 23-431. Either knockdown of NRP1 or pretreatment of EBV with soluble NRP1 suppresses EBV infection. Upregulation of NRP1 by overexpression or EGF treatment enhances EBV infection. However, NRP2, the homologue of NRP1, impairs EBV infection. EBV enters nasopharyngeal epithelial cells through NRP1-facilitated internalization and fusion, and through macropinocytosis and lipid raft-dependent endocytosis. NRP1 partially mediates EBV-activated EGFR/RAS/ERK signalling, and NRP1-dependent receptor tyrosine kinase (RTK) signalling promotes EBV infection. Taken together, NRP1 is identified as an EBV entry factor that cooperatively activates RTK signalling, which subsequently promotes EBV infection in nasopharyngeal epithelial cells. © 2014 Macmillan Publishers Limited. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/220087
ISSN
2015 Impact Factor: 11.329
2015 SCImago Journal Rankings: 6.539
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, HB-
dc.contributor.authorZhang, H-
dc.contributor.authorZhang, JP-
dc.contributor.authorLi, YL-
dc.contributor.authorZhao, B-
dc.contributor.authorFeng, GK-
dc.contributor.authorDu, Y-
dc.contributor.authorXiong, D-
dc.contributor.authorZhong, Q-
dc.contributor.authorLiu, WL-
dc.contributor.authorDu, H-
dc.contributor.authorLi, MZ-
dc.contributor.authorHuang, WL-
dc.contributor.authorTsao, GSW-
dc.contributor.authorHutt-Fletcher, L-
dc.contributor.authorZeng, YX-
dc.contributor.authorKieff, E-
dc.contributor.authorZeng, MS-
dc.date.accessioned2015-10-16T06:28:44Z-
dc.date.available2015-10-16T06:28:44Z-
dc.date.issued2015-
dc.identifier.citationNature Communications, 2015, v. 6, p. Article no. 6240-
dc.identifier.issn2041-1723-
dc.identifier.urihttp://hdl.handle.net/10722/220087-
dc.description.abstractEpstein-Barr virus (EBV) is implicated as an aetiological factor in B lymphomas and nasopharyngeal carcinoma. The mechanisms of cell-free EBV infection of nasopharyngeal epithelial cells remain elusive. EBV glycoprotein B (gB) is the critical fusion protein for infection of both B and epithelial cells, and determines EBV susceptibility of non-B cells. Here we show that neuropilin 1 (NRP1) directly interacts with EBV gB 23-431. Either knockdown of NRP1 or pretreatment of EBV with soluble NRP1 suppresses EBV infection. Upregulation of NRP1 by overexpression or EGF treatment enhances EBV infection. However, NRP2, the homologue of NRP1, impairs EBV infection. EBV enters nasopharyngeal epithelial cells through NRP1-facilitated internalization and fusion, and through macropinocytosis and lipid raft-dependent endocytosis. NRP1 partially mediates EBV-activated EGFR/RAS/ERK signalling, and NRP1-dependent receptor tyrosine kinase (RTK) signalling promotes EBV infection. Taken together, NRP1 is identified as an EBV entry factor that cooperatively activates RTK signalling, which subsequently promotes EBV infection in nasopharyngeal epithelial cells. © 2014 Macmillan Publishers Limited. All rights reserved.-
dc.languageeng-
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/ncomms/index.html-
dc.relation.ispartofNature Communications-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleNeuropilin 1 is an entry factor that promotes EBV infection of nasopharyngeal epithelial cells-
dc.typeArticle-
dc.identifier.emailTsao, GSW: gswtsao@hku.hk-
dc.identifier.authorityTsao, GSW=rp00399-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/ncomms7240-
dc.identifier.pmid25670642-
dc.identifier.pmcidPMC4339892-
dc.identifier.scopuseid_2-s2.0-84923108419-
dc.identifier.hkuros255640-
dc.identifier.volume6-
dc.identifier.spageArticle no. 6240-
dc.identifier.epageArticle no. 6240-
dc.identifier.isiWOS:000350202400004-
dc.publisher.placeUnited Kingdom-
dc.identifier.f1000725353089-

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