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Article: Nonmuscle myosin heavy chain IIA mediates Epstein-Barr virus infection of nasopharyngeal epithelial cells

TitleNonmuscle myosin heavy chain IIA mediates Epstein-Barr virus infection of nasopharyngeal epithelial cells
Authors
Issue Date2015
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings of the National Academy of Sciences, 2015, v. 112 n. 35, p. 11036-11041 How to Cite?
AbstractEBV causes B lymphomas and undifferentiated nasopharyngeal carcinoma (NPC). Although the mechanisms by which EBV infects B lymphocytes have been extensively studied, investigation of the mechanisms by which EBV infects nasopharyngeal epithelial cells (NPECs) has only recently been enabled by the successful growth of B lymphoma Mo-MLV insertion region 1 homolog (BMI1)-immortalized NPECs in vitro and the discovery that neuropilin 1 expression positively affects EBV glycoprotein B (gB)-mediated infection and tyrosine kinase activations in enhancing EBV infection of BMI1-immortalized NPECs. We have now found that even though EBV infected NPECs grown as a monolayer at extremely low efficiency (<3%), close to 30% of NPECs grown as sphere-like cells (SLCs) were infected by EBV. We also identified nonmuscle myosin heavy chain IIA (NMHC-IIA) as another NPEC protein important for efficient EBV infection. EBV gH/gL specifically interacted with NMHC-IIA both in vitro and in vivo. NMHC-IIA densely aggregated on the surface of NPEC SLCs and colocalized with EBV. EBV infection of NPEC SLCs was significantly reduced by NMHC-IIA siRNA knock-down. NMHC-IIA antisera also efficiently blocked EBV infection. These data indicate that NMHC-IIA is an important factor for EBV NPEC infection. © 2015 National Academy of Sciences. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/220049
ISSN
2015 Impact Factor: 9.423
2015 SCImago Journal Rankings: 6.883
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorXiong, D-
dc.contributor.authorDu, YL-
dc.contributor.authorWang, HB-
dc.contributor.authorZhao, B-
dc.contributor.authorZhang, HL-
dc.contributor.authorLi, Y-
dc.contributor.authorHu, LJ-
dc.contributor.authorCao, JY-
dc.contributor.authorZhong, Q-
dc.contributor.authorLiu, WL-
dc.contributor.authorLi, MZ-
dc.contributor.authorZhu, XF-
dc.contributor.authorTsao, GSW-
dc.contributor.authorHutt-Fletcher, LM-
dc.contributor.authorSong, E-
dc.contributor.authorZeng, YX-
dc.contributor.authorKieff, E-
dc.contributor.authorZeng, MS-
dc.date.accessioned2015-10-16T06:27:52Z-
dc.date.available2015-10-16T06:27:52Z-
dc.date.issued2015-
dc.identifier.citationProceedings of the National Academy of Sciences, 2015, v. 112 n. 35, p. 11036-11041-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/220049-
dc.description.abstractEBV causes B lymphomas and undifferentiated nasopharyngeal carcinoma (NPC). Although the mechanisms by which EBV infects B lymphocytes have been extensively studied, investigation of the mechanisms by which EBV infects nasopharyngeal epithelial cells (NPECs) has only recently been enabled by the successful growth of B lymphoma Mo-MLV insertion region 1 homolog (BMI1)-immortalized NPECs in vitro and the discovery that neuropilin 1 expression positively affects EBV glycoprotein B (gB)-mediated infection and tyrosine kinase activations in enhancing EBV infection of BMI1-immortalized NPECs. We have now found that even though EBV infected NPECs grown as a monolayer at extremely low efficiency (<3%), close to 30% of NPECs grown as sphere-like cells (SLCs) were infected by EBV. We also identified nonmuscle myosin heavy chain IIA (NMHC-IIA) as another NPEC protein important for efficient EBV infection. EBV gH/gL specifically interacted with NMHC-IIA both in vitro and in vivo. NMHC-IIA densely aggregated on the surface of NPEC SLCs and colocalized with EBV. EBV infection of NPEC SLCs was significantly reduced by NMHC-IIA siRNA knock-down. NMHC-IIA antisera also efficiently blocked EBV infection. These data indicate that NMHC-IIA is an important factor for EBV NPEC infection. © 2015 National Academy of Sciences. All rights reserved.-
dc.languageeng-
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org-
dc.relation.ispartofProceedings of the National Academy of Sciences-
dc.rightsProceedings of the National Academy of Sciences. Copyright © National Academy of Sciences.-
dc.titleNonmuscle myosin heavy chain IIA mediates Epstein-Barr virus infection of nasopharyngeal epithelial cells-
dc.typeArticle-
dc.identifier.emailTsao, GSW: gswtsao@hku.hk-
dc.identifier.authorityTsao, GSW=rp00399-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1073/pnas.1513359112-
dc.identifier.pmid26290577-
dc.identifier.pmcidPMC4577136-
dc.identifier.scopuseid_2-s2.0-84941013212-
dc.identifier.hkuros255637-
dc.identifier.volume112-
dc.identifier.issue35-
dc.identifier.spage11036-
dc.identifier.epage11041-
dc.identifier.isiWOS:000360383200064-
dc.publisher.placeUnited States-

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