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Conference Paper: Screening and mechanistic study of estradiol-stimulating fractions derived from erxian decoction in aged sprague-dawley rats

TitleScreening and mechanistic study of estradiol-stimulating fractions derived from erxian decoction in aged sprague-dawley rats
促進老年SD 大鼠卵巢雌激素合成的二仙湯活性組分篩選和機理研究
Authors
Issue Date2014
Citation
The 8th Pong Ding Yuen International Symposium on Traditional Chinese Medicine cum The 2nd International Chinese Symposium on Free Radical Research & The 6th Symposium for Three Districts of Cross-straits on Free Radical Research, The University of Hong Kong, Hong Kong, 15-16 November 2014. How to Cite?
AbstractBACKGROUND: Erxian Decoction (EXD), a famous herbal formula, has been used to relieve menopausal syndrome for more than 60 years. Our previous study firstly reported that EXD could promote estradiol biosynthesis in vivo via up-regulation of ovarian aromatase, but it is still unknown which components from EXD promote estradiol biosynthesis. OBJECTIVES: To screen the effective fraction extracted from EXD which promotes estradiol biosynthesis in vivo. METHODS: Four bioactive fractions derived from aqueous EXD decoction, i.e. polysaccharide fraction (PS), ethyl acetate fraction (EA), n-butanol fraction (BU) and water faction (WA), were subsequently extracted by solvent partition method. The polysaccharide fraction (PS) was obtained from the total aqueous decoction (TW) of EXD by precipitation with 75% ethanol overnight at 4℃. After filtration, the residue was the total EXD ethanol extract. The total EXD ethanol extract was extracted with three volumes of ethyl acetate at room temperature to yield the EA. The fraction which remained was processed with three volumes of n-butanol at room temperature to yield the BU and the fraction which remained was WA. Each of the above fractions (PS, EA, BU and WA) together with the EXD (TW) were dried to powder form using a freezer dryer. Then the powders from the five fractions were dissolved in Milli-Q water for oral administration into SD rats (≧ 18 months) for 4 weeks. The concentrations of powders per body weight of SD-rat were calculated according to their recovery rates, they are shown as follows: TW = 4.10g/kg, PS = 1.64g/kg, EA = 0.11g/kg, BU = 0.47g/kg and WA = 2.34g/kg. RESULTS: The serum estradiol level in PS-treated aged SD-rats was the highest compared with other groups. PS also enhanced the protein expression of FSHR and aromatase in the ovaries of SD rats. CONCLUSION: Polysaccharide is the main component from EXD which stimulated estradiol biosynthesis. Its mechanism is mainly via up-regulating the FSHR and aromatase expression in the ovary. Thus polysaccharide from EXD could be used as an alternative treatment for relieving menopausal syndrome.
DescriptionCode: YI-12/P-53
Persistent Identifierhttp://hdl.handle.net/10722/218060

 

DC FieldValueLanguage
dc.contributor.authorWang, S-
dc.contributor.authorCheung, HP-
dc.contributor.authorNg, TB-
dc.contributor.authorLao, L-
dc.contributor.authorZhang, Y-
dc.contributor.authorSze, CWS-
dc.date.accessioned2015-09-18T06:21:52Z-
dc.date.available2015-09-18T06:21:52Z-
dc.date.issued2014-
dc.identifier.citationThe 8th Pong Ding Yuen International Symposium on Traditional Chinese Medicine cum The 2nd International Chinese Symposium on Free Radical Research & The 6th Symposium for Three Districts of Cross-straits on Free Radical Research, The University of Hong Kong, Hong Kong, 15-16 November 2014.-
dc.identifier.urihttp://hdl.handle.net/10722/218060-
dc.descriptionCode: YI-12/P-53-
dc.description.abstractBACKGROUND: Erxian Decoction (EXD), a famous herbal formula, has been used to relieve menopausal syndrome for more than 60 years. Our previous study firstly reported that EXD could promote estradiol biosynthesis in vivo via up-regulation of ovarian aromatase, but it is still unknown which components from EXD promote estradiol biosynthesis. OBJECTIVES: To screen the effective fraction extracted from EXD which promotes estradiol biosynthesis in vivo. METHODS: Four bioactive fractions derived from aqueous EXD decoction, i.e. polysaccharide fraction (PS), ethyl acetate fraction (EA), n-butanol fraction (BU) and water faction (WA), were subsequently extracted by solvent partition method. The polysaccharide fraction (PS) was obtained from the total aqueous decoction (TW) of EXD by precipitation with 75% ethanol overnight at 4℃. After filtration, the residue was the total EXD ethanol extract. The total EXD ethanol extract was extracted with three volumes of ethyl acetate at room temperature to yield the EA. The fraction which remained was processed with three volumes of n-butanol at room temperature to yield the BU and the fraction which remained was WA. Each of the above fractions (PS, EA, BU and WA) together with the EXD (TW) were dried to powder form using a freezer dryer. Then the powders from the five fractions were dissolved in Milli-Q water for oral administration into SD rats (≧ 18 months) for 4 weeks. The concentrations of powders per body weight of SD-rat were calculated according to their recovery rates, they are shown as follows: TW = 4.10g/kg, PS = 1.64g/kg, EA = 0.11g/kg, BU = 0.47g/kg and WA = 2.34g/kg. RESULTS: The serum estradiol level in PS-treated aged SD-rats was the highest compared with other groups. PS also enhanced the protein expression of FSHR and aromatase in the ovaries of SD rats. CONCLUSION: Polysaccharide is the main component from EXD which stimulated estradiol biosynthesis. Its mechanism is mainly via up-regulating the FSHR and aromatase expression in the ovary. Thus polysaccharide from EXD could be used as an alternative treatment for relieving menopausal syndrome.-
dc.languageeng-
dc.relation.ispartof8th Pong Ding Yuen International Symposium on Traditional Chinese Medicine, 2nd International Chinese Symposium on Free Radical Research & 6th Symposium for Three Districts of Cross-straits on Free Radical Research-
dc.relation.ispartof第八屇龐鼎元國際中醫藥研討會暨第二屇世界華人自由基生物學-
dc.titleScreening and mechanistic study of estradiol-stimulating fractions derived from erxian decoction in aged sprague-dawley rats-
dc.title促進老年SD 大鼠卵巢雌激素合成的二仙湯活性組分篩選和機理研究-
dc.typeConference_Paper-
dc.identifier.emailLao, L: lxlao1@hku.hk-
dc.identifier.emailZhang, Y: ybzhang@hku.hk-
dc.identifier.emailSze, CWS: stephens@hku.hk-
dc.identifier.authorityLao, L=rp01784-
dc.identifier.authorityZhang, Y=rp01410-
dc.identifier.authoritySze, CWS=rp00514-
dc.identifier.hkuros253097-

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