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Conference Paper: Accelerated maturation of hESC-CM within collagen-based 3D matrix upon niche cell supplementation and mechanical stretch

TitleAccelerated maturation of hESC-CM within collagen-based 3D matrix upon niche cell supplementation and mechanical stretch
Authors
KeywordsBiology
Bioengineering
Issue Date2015
PublisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=263
Citation
The 4th World Congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS 2015), Boston, MA., 8-11 September 2015. In Tissue Engineering Part A, 2015, v. 21 n. S1, p. S326-S327 How to Cite?
AbstractRegenerate injured myocardium with cardiomyocytes derived from stem cells is a promising strategy formyocardial infarction patients, yet maturation of those stem cell derived cardiomyocytes is still one of the main obstacles for clinical application. Facilitated maturation in vitro could be achieved by tissue engineering technology, where stem cells further functionalize under physiological-mimicking stimuli within 3D matrix. Here we investigated the parameters with the potential to promote maturation of human embryonic stem cell derived cardiomyocytes (hESC-CM) within 3D collagen matrix. After harvest cardiospheres differentiated from hESCs between D20 and D30, engineered cardiac tissue (ECT) was fabricated by coencapsulating digested hESC-CMs with 5% hMSC/hFB in collagen gel and cast into customized PDMS mold, it was allowed to selfassemble into a solid tissue strip with fixed length for 7 days, static or cyclic stretch was applied afterwards. Maturation status of those ECTs was evaluated by qPCR, immunostaining and mechanical properties. Niche cell supplementation was found to augment expression of contractile proteins, hypertrophic genes as well as those in change with calcium handling. Moreover, spreading, modulus and beating metrics were boosted with auxiliary cells as well. Although to a less extent, stretching also significantly impact the maturation with upregulated expression of Cx45, BNP&SERCA2, better alignment and greater twitch force of ECTs. The current study demonstrated that maturation of hESC-CMECT could be accelerated by the addition of niche cells as low as 5%, while tensional loading could further strengthen the artificial tissue strip into better functional cardiac muscle for regenerative medicine.
DescriptionCongress Theme: Past, Present, Future: the evolution of Regenerative Medicine
Poster abstracts
Persistent Identifierhttp://hdl.handle.net/10722/217477
ISSN
2015 SCImago Journal Rankings: 1.500

 

DC FieldValueLanguage
dc.contributor.authorZHANG, W-
dc.contributor.authorKong, CW-
dc.contributor.authorLi, RA-
dc.contributor.authorChan, BP-
dc.date.accessioned2015-09-18T06:00:26Z-
dc.date.available2015-09-18T06:00:26Z-
dc.date.issued2015-
dc.identifier.citationThe 4th World Congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS 2015), Boston, MA., 8-11 September 2015. In Tissue Engineering Part A, 2015, v. 21 n. S1, p. S326-S327-
dc.identifier.issn1937-3341-
dc.identifier.urihttp://hdl.handle.net/10722/217477-
dc.descriptionCongress Theme: Past, Present, Future: the evolution of Regenerative Medicine-
dc.descriptionPoster abstracts-
dc.description.abstractRegenerate injured myocardium with cardiomyocytes derived from stem cells is a promising strategy formyocardial infarction patients, yet maturation of those stem cell derived cardiomyocytes is still one of the main obstacles for clinical application. Facilitated maturation in vitro could be achieved by tissue engineering technology, where stem cells further functionalize under physiological-mimicking stimuli within 3D matrix. Here we investigated the parameters with the potential to promote maturation of human embryonic stem cell derived cardiomyocytes (hESC-CM) within 3D collagen matrix. After harvest cardiospheres differentiated from hESCs between D20 and D30, engineered cardiac tissue (ECT) was fabricated by coencapsulating digested hESC-CMs with 5% hMSC/hFB in collagen gel and cast into customized PDMS mold, it was allowed to selfassemble into a solid tissue strip with fixed length for 7 days, static or cyclic stretch was applied afterwards. Maturation status of those ECTs was evaluated by qPCR, immunostaining and mechanical properties. Niche cell supplementation was found to augment expression of contractile proteins, hypertrophic genes as well as those in change with calcium handling. Moreover, spreading, modulus and beating metrics were boosted with auxiliary cells as well. Although to a less extent, stretching also significantly impact the maturation with upregulated expression of Cx45, BNP&SERCA2, better alignment and greater twitch force of ECTs. The current study demonstrated that maturation of hESC-CMECT could be accelerated by the addition of niche cells as low as 5%, while tensional loading could further strengthen the artificial tissue strip into better functional cardiac muscle for regenerative medicine.-
dc.languageeng-
dc.publisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=263-
dc.relation.ispartofTissue Engineering Part A: Tissue Engineering-
dc.rightsTissue Engineering Part A: Tissue Engineering. Copyright © Mary Ann Liebert, Inc. Publishers.-
dc.subjectBiology-
dc.subjectBioengineering-
dc.titleAccelerated maturation of hESC-CM within collagen-based 3D matrix upon niche cell supplementation and mechanical stretch-
dc.typeConference_Paper-
dc.identifier.emailKong, CW: mkcwkong@hku.hk-
dc.identifier.emailLi, RA: ronaldli@hkucc.hku.hk-
dc.identifier.emailChan, BP: bpchan@hku.hk-
dc.identifier.authorityKong, CW=rp01563-
dc.identifier.authorityLi, RA=rp01352-
dc.identifier.authorityChan, BP=rp00087-
dc.identifier.hkuros251893-
dc.identifier.volume21-
dc.identifier.issueS1-
dc.identifier.spageS-326-
dc.identifier.epageS-327-
dc.publisher.placeUnited States-

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