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Article: Monodisperse Emulsion Drop Microenvironments for Bacterial Biofilm Growth

TitleMonodisperse Emulsion Drop Microenvironments for Bacterial Biofilm Growth
Authors
Issue Date2015
PublisherWiley. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jabout/107640323/2421_info.html
Citation
Small, 2015, v. 11, p. 3954 How to Cite?
AbstractIn this work, microfluidic technology is used to rapidly create hundreds of thousands of monodisperse double and triple emulsion drops that serve as 3D microenvironments for the containment and growth of bacterial biofilms. The size of these drops, with diameters from tens to hundreds of micrometers, makes them amenable to rapid manipulation and analysis. This is demonstrated by using microscopy to visualize cellular differentiation of Bacillus subtilis biofilm communities within each drop and the bacterial biofilm microstructure. Biofilm growth is explored upon specific interfaces in double and triple emulsions and upon negative and positive radii of curvature. Biofilm attachment of matrix and flagella mutants is studied as well as biofilms of Pseudomonas aeruginosa. This is the first demonstration of biofilms grown in microscale emulsion drops, which serve as both templates and containers for biofilm growth and attachment. These microenvironments have the potential to transform existing high-throughput screening methods for bacterial biofilms.
Persistent Identifierhttp://hdl.handle.net/10722/217082

 

DC FieldValueLanguage
dc.contributor.authorChang, CB-
dc.contributor.authorWilking, JN-
dc.contributor.authorKim, SH-
dc.contributor.authorShum, HC-
dc.contributor.authorWeitz, DA-
dc.date.accessioned2015-09-18T05:47:41Z-
dc.date.available2015-09-18T05:47:41Z-
dc.date.issued2015-
dc.identifier.citationSmall, 2015, v. 11, p. 3954-
dc.identifier.urihttp://hdl.handle.net/10722/217082-
dc.description.abstractIn this work, microfluidic technology is used to rapidly create hundreds of thousands of monodisperse double and triple emulsion drops that serve as 3D microenvironments for the containment and growth of bacterial biofilms. The size of these drops, with diameters from tens to hundreds of micrometers, makes them amenable to rapid manipulation and analysis. This is demonstrated by using microscopy to visualize cellular differentiation of Bacillus subtilis biofilm communities within each drop and the bacterial biofilm microstructure. Biofilm growth is explored upon specific interfaces in double and triple emulsions and upon negative and positive radii of curvature. Biofilm attachment of matrix and flagella mutants is studied as well as biofilms of Pseudomonas aeruginosa. This is the first demonstration of biofilms grown in microscale emulsion drops, which serve as both templates and containers for biofilm growth and attachment. These microenvironments have the potential to transform existing high-throughput screening methods for bacterial biofilms.-
dc.languageeng-
dc.publisherWiley. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jabout/107640323/2421_info.html-
dc.relation.ispartofSmall-
dc.rightsPreprint This is the pre-peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article]. Authors are not required to remove preprints posted prior to acceptance of the submitted version. Postprint This is the accepted version of the following article: [full citation], which has been published in final form at [Link to final article]. In addition, authors may also transmit, print and share copies with colleagues, provided that there is no systematic distribution of the submitted version, e.g. posting on a listserve, network or automated delivery. -
dc.titleMonodisperse Emulsion Drop Microenvironments for Bacterial Biofilm Growth-
dc.typeArticle-
dc.identifier.emailShum, HC: ashum@hku.hk-
dc.identifier.authorityShum, HC=rp01439-
dc.identifier.hkuros250692-
dc.identifier.volume11-
dc.identifier.spage3954-
dc.identifier.epage3954-

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