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Conference Paper: Immobilization of chondroitinase ABC-I and -II on chitosan microbeads improve neurite growth in CSPG-enriched astrocyte culture

TitleImmobilization of chondroitinase ABC-I and -II on chitosan microbeads improve neurite growth in CSPG-enriched astrocyte culture
Authors
Issue Date2015
Citation
The 10th International Symposium on Healthy Aging, Hong Kong, 7-8 March 2015. How to Cite?
AbstractChondroitin sulfate proteoglycans (CSPGs) are extra-cellular matrix molecules that are upregulated at lesion site restricting axonal regrowth after spinal cord injury. Recombinant chondroitinase ABC I and II (ChABC-I & -II) enhance prospects of axonal regrowth through the lesion by cleaving CS moieties of the PGs. We attempted to immobilize ChABC-I or -II separately on chitosan microbeads using glutaric dialdehyde to reduce ChABC activity decay in vivo. Immobilized ChABC-I demonstrated CS-cleaving activity both in biochemical assay and in CSPGs-enriched astrocyte cultures that had been activated by transforming growth factor beta (TGF-β). Neurite length was increased in co-cultures of TGF-!b activated astrocytes and cortical neurons mixed with immobilized ChABC-I or immobilized ChABC-II. Given CSPG enrichment at astrocyte-Schwann cell (A/S) encounters, A/S confrontation co-culture was used in a further bioassay of immobilized enzyme activity. Preliminary data showed that neurite length was increased in ChABC-I treated A/S co-culture. In future, the effect of immobilized ChABC-I and -II on neurite length will be tested in A/S co-culture.
DescriptionConference Theme: A Decade of Positive Aging
Persistent Identifierhttp://hdl.handle.net/10722/216630

 

DC FieldValueLanguage
dc.contributor.authorKwok, LF-
dc.contributor.authorTam, KW-
dc.contributor.authorChan, YS-
dc.contributor.authorShum, DKY-
dc.date.accessioned2015-09-18T05:33:58Z-
dc.date.available2015-09-18T05:33:58Z-
dc.date.issued2015-
dc.identifier.citationThe 10th International Symposium on Healthy Aging, Hong Kong, 7-8 March 2015.-
dc.identifier.urihttp://hdl.handle.net/10722/216630-
dc.descriptionConference Theme: A Decade of Positive Aging-
dc.description.abstractChondroitin sulfate proteoglycans (CSPGs) are extra-cellular matrix molecules that are upregulated at lesion site restricting axonal regrowth after spinal cord injury. Recombinant chondroitinase ABC I and II (ChABC-I & -II) enhance prospects of axonal regrowth through the lesion by cleaving CS moieties of the PGs. We attempted to immobilize ChABC-I or -II separately on chitosan microbeads using glutaric dialdehyde to reduce ChABC activity decay in vivo. Immobilized ChABC-I demonstrated CS-cleaving activity both in biochemical assay and in CSPGs-enriched astrocyte cultures that had been activated by transforming growth factor beta (TGF-β). Neurite length was increased in co-cultures of TGF-!b activated astrocytes and cortical neurons mixed with immobilized ChABC-I or immobilized ChABC-II. Given CSPG enrichment at astrocyte-Schwann cell (A/S) encounters, A/S confrontation co-culture was used in a further bioassay of immobilized enzyme activity. Preliminary data showed that neurite length was increased in ChABC-I treated A/S co-culture. In future, the effect of immobilized ChABC-I and -II on neurite length will be tested in A/S co-culture.-
dc.languageeng-
dc.relation.ispartofInternational Symposium on Healthy Aging-
dc.titleImmobilization of chondroitinase ABC-I and -II on chitosan microbeads improve neurite growth in CSPG-enriched astrocyte culture-
dc.typeConference_Paper-
dc.identifier.emailKwok, LF: lamfungs@hku.hk-
dc.identifier.emailTam, KW: tamkw@hku.hk-
dc.identifier.emailChan, YS: yschan@hku.hk-
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hk-
dc.identifier.authorityChan, YS=rp00318-
dc.identifier.authorityShum, DKY=rp00321-
dc.identifier.hkuros253273-

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