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Conference Paper: Immobilization of chondroitinase ABC-I and -II on chitosan microbeads improve neurite growth in CSPG-enriched astrocyte culture
Title | Immobilization of chondroitinase ABC-I and -II on chitosan microbeads improve neurite growth in CSPG-enriched astrocyte culture |
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Authors | |
Issue Date | 2015 |
Citation | The 10th International Symposium on Healthy Aging (ISHA 2015), Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 7-8 March 2015. How to Cite? |
Abstract | Chondroitin sulfate proteoglycans (CSPGs) are extra-cellular matrix molecules that are upregulated at lesion site restricting axonal regrowth after spinal cord injury. Recombinant chondroitinase ABC I and II (ChABC-I & -II) enhance prospects of axonal regrowth through the lesion by cleaving CS moieties of the PGs. We attempted to immobilize ChABC-I or -II separately on chitosan microbeads using glutaric dialdehyde to reduce ChABC activity decay in vivo. Immobilized ChABC-I demonstrated CS-cleaving activity both in biochemical assay and in CSPGs-enriched astrocyte cultures that had been activated by transforming growth factor beta (TGF-β). Neurite length was increased in co-cultures of TGF-!b activated astrocytes and cortical neurons mixed with immobilized ChABC-I or immobilized ChABC-II. Given CSPG enrichment at astrocyte-Schwann cell (A/S) encounters, A/S confrontation co-culture was used in a further bioassay of immobilized enzyme activity. Preliminary data showed that neurite length was increased in ChABC-I treated A/S co-culture. In future, the effect of immobilized ChABC-I and -II on neurite length will be tested in A/S co-culture. |
Description | Conference Theme: A Decade of Positive Aging |
Persistent Identifier | http://hdl.handle.net/10722/216630 |
DC Field | Value | Language |
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dc.contributor.author | Kwok, LF | - |
dc.contributor.author | Tam, KW | - |
dc.contributor.author | Chan, YS | - |
dc.contributor.author | Shum, DKY | - |
dc.date.accessioned | 2015-09-18T05:33:58Z | - |
dc.date.available | 2015-09-18T05:33:58Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | The 10th International Symposium on Healthy Aging (ISHA 2015), Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 7-8 March 2015. | - |
dc.identifier.uri | http://hdl.handle.net/10722/216630 | - |
dc.description | Conference Theme: A Decade of Positive Aging | - |
dc.description.abstract | Chondroitin sulfate proteoglycans (CSPGs) are extra-cellular matrix molecules that are upregulated at lesion site restricting axonal regrowth after spinal cord injury. Recombinant chondroitinase ABC I and II (ChABC-I & -II) enhance prospects of axonal regrowth through the lesion by cleaving CS moieties of the PGs. We attempted to immobilize ChABC-I or -II separately on chitosan microbeads using glutaric dialdehyde to reduce ChABC activity decay in vivo. Immobilized ChABC-I demonstrated CS-cleaving activity both in biochemical assay and in CSPGs-enriched astrocyte cultures that had been activated by transforming growth factor beta (TGF-β). Neurite length was increased in co-cultures of TGF-!b activated astrocytes and cortical neurons mixed with immobilized ChABC-I or immobilized ChABC-II. Given CSPG enrichment at astrocyte-Schwann cell (A/S) encounters, A/S confrontation co-culture was used in a further bioassay of immobilized enzyme activity. Preliminary data showed that neurite length was increased in ChABC-I treated A/S co-culture. In future, the effect of immobilized ChABC-I and -II on neurite length will be tested in A/S co-culture. | - |
dc.language | eng | - |
dc.relation.ispartof | International Symposium on Healthy Aging, ISHA 2015 | - |
dc.title | Immobilization of chondroitinase ABC-I and -II on chitosan microbeads improve neurite growth in CSPG-enriched astrocyte culture | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Kwok, LF: lamfungs@hku.hk | - |
dc.identifier.email | Tam, KW: tamkw@hku.hk | - |
dc.identifier.email | Chan, YS: yschan@hku.hk | - |
dc.identifier.email | Shum, DKY: shumdkhk@hkucc.hku.hk | - |
dc.identifier.authority | Chan, YS=rp00318 | - |
dc.identifier.authority | Shum, DKY=rp00321 | - |
dc.identifier.hkuros | 253273 | - |
dc.identifier.hkuros | 254778 | - |