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Conference Paper: Long-chain inorganic polyphosphate is highly enriched in osteoblastic matrix vesicles

TitleLong-chain inorganic polyphosphate is highly enriched in osteoblastic matrix vesicles
Authors
KeywordsBiology
Biochemistry
Issue Date2015
PublisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.febsjournal.org/
Citation
The 40th FEBS Congress 2015, Berlin, Germany, 4-9 July 2015. In FEBS Journal, 2015, v. 282 suppl. S1, p. 101-102, abstract no. P08-016 How to Cite?
AbstractEvidence is emerging that long-chain inorganic polyphosphates play a variety of critical roles in mammalian cells that remain little understood. In particular it has been posited that polyphosphate is found at a relatively high concentration in osteoblasts but many unanswered questions remain regarding polyphosphate function and synthesis. One significant challenge is the lack of tools to observe and analyse polyphosphate function. Here, we report evidence that polyphosphate is highly enriched in SaOS-2 osteosarcoma osteoblastic matrix vesicles. In one line of evidence, we use various chromatographic approaches coupled to use of DAPI as a wavelength-dependent polyphosphate specific dye. In a second line of evidence, we use a heavy metal labelled polyphosphate binding domain (PPBD) of Escherichia coli exopolyphosphatase (PPX) as a tool for the observation of polyphosphate by transmission electron microscopy. Results correlated in observing a particularly strong enrichment of polyphosphate in osteoblastic matrix vesicles. Polyphosphates likely play important roles in mineralization in matrix vesicles, suggesting polyphosphate could be an overlooked key molecule in extracellular matrix biology.
DescriptionCongress Theme: The Biochemical Basis of Life
Poster Sessions: P08-016
This free journal suppl. entitled: Special Issue: 40th FEBS Congress, The Biochemical Basis of Life, Berlin, Germany, July 4-9, 2015
Persistent Identifierhttp://hdl.handle.net/10722/216627
ISSN
2015 Impact Factor: 4.237
2015 SCImago Journal Rankings: 2.141

 

DC FieldValueLanguage
dc.contributor.authorLi, L-
dc.contributor.authorKhong, ML-
dc.contributor.authorLu, B-
dc.contributor.authorWatt, RM-
dc.contributor.authorTanner, JA-
dc.date.accessioned2015-09-18T05:33:54Z-
dc.date.available2015-09-18T05:33:54Z-
dc.date.issued2015-
dc.identifier.citationThe 40th FEBS Congress 2015, Berlin, Germany, 4-9 July 2015. In FEBS Journal, 2015, v. 282 suppl. S1, p. 101-102, abstract no. P08-016-
dc.identifier.issn1742-464X-
dc.identifier.urihttp://hdl.handle.net/10722/216627-
dc.descriptionCongress Theme: The Biochemical Basis of Life-
dc.descriptionPoster Sessions: P08-016-
dc.descriptionThis free journal suppl. entitled: Special Issue: 40th FEBS Congress, The Biochemical Basis of Life, Berlin, Germany, July 4-9, 2015-
dc.description.abstractEvidence is emerging that long-chain inorganic polyphosphates play a variety of critical roles in mammalian cells that remain little understood. In particular it has been posited that polyphosphate is found at a relatively high concentration in osteoblasts but many unanswered questions remain regarding polyphosphate function and synthesis. One significant challenge is the lack of tools to observe and analyse polyphosphate function. Here, we report evidence that polyphosphate is highly enriched in SaOS-2 osteosarcoma osteoblastic matrix vesicles. In one line of evidence, we use various chromatographic approaches coupled to use of DAPI as a wavelength-dependent polyphosphate specific dye. In a second line of evidence, we use a heavy metal labelled polyphosphate binding domain (PPBD) of Escherichia coli exopolyphosphatase (PPX) as a tool for the observation of polyphosphate by transmission electron microscopy. Results correlated in observing a particularly strong enrichment of polyphosphate in osteoblastic matrix vesicles. Polyphosphates likely play important roles in mineralization in matrix vesicles, suggesting polyphosphate could be an overlooked key molecule in extracellular matrix biology.-
dc.languageeng-
dc.publisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.febsjournal.org/-
dc.relation.ispartofThe FEBS Journal-
dc.rightsPreprint This is the pre-peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article]. Authors are not required to remove preprints posted prior to acceptance of the submitted version. Postprint This is the accepted version of the following article: [full citation], which has been published in final form at [Link to final article].-
dc.subjectBiology-
dc.subjectBiochemistry-
dc.titleLong-chain inorganic polyphosphate is highly enriched in osteoblastic matrix vesicles-
dc.typeConference_Paper-
dc.identifier.emailLi, L: linali@hku.hk-
dc.identifier.emailWatt, RM: rmwatt@hku.hk-
dc.identifier.emailTanner, JA: jatanner@hkucc.hku.hk-
dc.identifier.authorityWatt, RM=rp00043-
dc.identifier.authorityTanner, JA=rp00495-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1111/febs.13339-
dc.identifier.hkuros253081-
dc.identifier.volume282-
dc.identifier.issuesuppl. S1-
dc.identifier.spage101, abstract no. P08-
dc.identifier.epage102-
dc.publisher.placeUnited Kingdom-

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