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Conference Paper: DNA aptamers for malaria diagnosis - from crystal structure to clinical application

TitleDNA aptamers for malaria diagnosis - from crystal structure to clinical application
Authors
KeywordsBiology
Biochemistry
Issue Date2015
PublisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.febsjournal.org/
Citation
The 40th FEBS Congress 2015, Berlin, Germany, 4-9 July 2015. In FEBS Journal, 2015, v. 282 suppl. S1, p. 344-345, abstract no. P36-023 How to Cite?
AbstractDNA aptamers have the potential to disrupt medical diagnostics by replacing antibodies for molecular recognition. However, few aptamers have reached the stage of clinical application. A key challenge is discovery of suitable aptamer-target pairs and how best to link the binding event to a diagnostic signal particularly for point-of-care tests. Here, we present our work to develop DNA aptamers for point-of-care malaria diagnosis. We discovered aptamers against Plasmodium lactate dehydrogenase as a general Plasmodium biomarker and against histidine-rich protein 2 as a Plasmodium falciparum specific biomarker. We have solved the crystal structure of Plasmodium lactate dehydrogenase aptamer in complex with its target, making this one of the best characterized aptamer-target pairs. Furthermore, we have integrated aptamers into an assay termed aptamer-tethered enzyme capture (APTEC) which is able to diagnose malaria in stored clinical patient blood samples. Work is ongoing using microarrays to optimize aptamer affinity and we are rapid prototyping 3D printed aptamer-enabled devices with a view to developing a point-of-care diagnostic test that is inexpensive, robust, sensitive and specific for the developing world.
DescriptionCongress Theme: The Biochemical Basis of Life
Poster Sessions: P36-023
This free journal suppl. entitled: Special Issue: 40th FEBS Congress, The Biochemical Basis of Life, Berlin, Germany, July 4-9, 2015
Persistent Identifierhttp://hdl.handle.net/10722/216626
ISSN
2015 Impact Factor: 4.237
2015 SCImago Journal Rankings: 2.141

 

DC FieldValueLanguage
dc.contributor.authorCheung, YW-
dc.contributor.authorDirkzwager, RM-
dc.contributor.authorKinghorn, AB-
dc.contributor.authorFraser, LA-
dc.contributor.authorLiang, S-
dc.contributor.authorTang, MSL-
dc.contributor.authorKotaka, M-
dc.contributor.authorRichards, JS-
dc.contributor.authorTanner, JA-
dc.date.accessioned2015-09-18T05:33:53Z-
dc.date.available2015-09-18T05:33:53Z-
dc.date.issued2015-
dc.identifier.citationThe 40th FEBS Congress 2015, Berlin, Germany, 4-9 July 2015. In FEBS Journal, 2015, v. 282 suppl. S1, p. 344-345, abstract no. P36-023-
dc.identifier.issn1742-464X-
dc.identifier.urihttp://hdl.handle.net/10722/216626-
dc.descriptionCongress Theme: The Biochemical Basis of Life-
dc.descriptionPoster Sessions: P36-023-
dc.descriptionThis free journal suppl. entitled: Special Issue: 40th FEBS Congress, The Biochemical Basis of Life, Berlin, Germany, July 4-9, 2015-
dc.description.abstractDNA aptamers have the potential to disrupt medical diagnostics by replacing antibodies for molecular recognition. However, few aptamers have reached the stage of clinical application. A key challenge is discovery of suitable aptamer-target pairs and how best to link the binding event to a diagnostic signal particularly for point-of-care tests. Here, we present our work to develop DNA aptamers for point-of-care malaria diagnosis. We discovered aptamers against Plasmodium lactate dehydrogenase as a general Plasmodium biomarker and against histidine-rich protein 2 as a Plasmodium falciparum specific biomarker. We have solved the crystal structure of Plasmodium lactate dehydrogenase aptamer in complex with its target, making this one of the best characterized aptamer-target pairs. Furthermore, we have integrated aptamers into an assay termed aptamer-tethered enzyme capture (APTEC) which is able to diagnose malaria in stored clinical patient blood samples. Work is ongoing using microarrays to optimize aptamer affinity and we are rapid prototyping 3D printed aptamer-enabled devices with a view to developing a point-of-care diagnostic test that is inexpensive, robust, sensitive and specific for the developing world.-
dc.languageeng-
dc.publisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.febsjournal.org/-
dc.relation.ispartofThe FEBS Journal-
dc.rightsPreprint This is the pre-peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article]. Authors are not required to remove preprints posted prior to acceptance of the submitted version. Postprint This is the accepted version of the following article: [full citation], which has been published in final form at [Link to final article].-
dc.subjectBiology-
dc.subjectBiochemistry-
dc.titleDNA aptamers for malaria diagnosis - from crystal structure to clinical application-
dc.typeConference_Paper-
dc.identifier.emailCheung, YW: cheungw@hku.hk-
dc.identifier.emailTanner, JA: jatanner@hkucc.hku.hk-
dc.identifier.authorityTanner, JA=rp00495-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1111/febs.13339-
dc.identifier.hkuros253080-
dc.identifier.volume282-
dc.identifier.issuesuppl. S1-
dc.identifier.spage344, abstract no. P36-
dc.identifier.epage345-
dc.publisher.placeUnited Kingdom-

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