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Article: Keratinocyte-fibroblast paracrine interaction: The effects of substrate and culture condition

TitleKeratinocyte-fibroblast paracrine interaction: The effects of substrate and culture condition
Authors
KeywordsPEG
Hydrogels
huKGF
Fibroblast co-culture
Cell adhesion
Issue Date2005
Citation
Biomaterials, 2005, v. 26, n. 17, p. 3673-3682 How to Cite?
AbstractInteractions between epidermal-dermal cells via soluble factors provide important signals in regulating the reepithelialization of wounded skin. For example, keratinocytes regulate the expression of keratinocyte growth factor (KGF) in fibroblasts through the release of interleukin-1beta (IL-1β). In this study, a previously developed polyethyleneglycol-based interpenetrating network (IPN) system was utilized as a platform for the delivery of keratinocyte-active factors. The effect of substrate chemistry, culture condition, and the delivery of exogenous keratinocyte-active factors on the keratinocyte behavior and the keratinocyte-fibroblast paracrine relationship was delineated. Adherent keratinocyte density on TCPS and glutaraldehyde-fixed gelatin hydrogels but not on IPN was significantly increased with culture time in the presence of growth supplements independent of the released KGF from the gelatin hydrogel and IPN. In the presence of fibroblasts, adherent keratinocyte density on gelatin hydrogels was higher than that without fibroblasts. This phenomenon was not observed on IPN and polycarbonate membrane. In summary, the delivered exogenous huKGF (i.e., released from a biomaterial matrix) operates in tandem with fibroblasts in regulating keratinocyte activation (i.e., IL-lβ release and adhesion) in a surface-dependent manner. Immunoassay analysis of cell culture keratinocyte-fibroblast paracrine relationship as characterized by IL-1β and KGF could not be established in the presence of IPNs, 0.1% glutaraldehyde-fixed gelatin hydrogels, and polycarbonate membranes. © 2004 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/216172
ISSN
2015 Impact Factor: 8.387
2015 SCImago Journal Rankings: 3.565

 

DC FieldValueLanguage
dc.contributor.authorWitte, Richard P.-
dc.contributor.authorKao, Weiyuan John-
dc.date.accessioned2015-08-25T10:22:10Z-
dc.date.available2015-08-25T10:22:10Z-
dc.date.issued2005-
dc.identifier.citationBiomaterials, 2005, v. 26, n. 17, p. 3673-3682-
dc.identifier.issn0142-9612-
dc.identifier.urihttp://hdl.handle.net/10722/216172-
dc.description.abstractInteractions between epidermal-dermal cells via soluble factors provide important signals in regulating the reepithelialization of wounded skin. For example, keratinocytes regulate the expression of keratinocyte growth factor (KGF) in fibroblasts through the release of interleukin-1beta (IL-1β). In this study, a previously developed polyethyleneglycol-based interpenetrating network (IPN) system was utilized as a platform for the delivery of keratinocyte-active factors. The effect of substrate chemistry, culture condition, and the delivery of exogenous keratinocyte-active factors on the keratinocyte behavior and the keratinocyte-fibroblast paracrine relationship was delineated. Adherent keratinocyte density on TCPS and glutaraldehyde-fixed gelatin hydrogels but not on IPN was significantly increased with culture time in the presence of growth supplements independent of the released KGF from the gelatin hydrogel and IPN. In the presence of fibroblasts, adherent keratinocyte density on gelatin hydrogels was higher than that without fibroblasts. This phenomenon was not observed on IPN and polycarbonate membrane. In summary, the delivered exogenous huKGF (i.e., released from a biomaterial matrix) operates in tandem with fibroblasts in regulating keratinocyte activation (i.e., IL-lβ release and adhesion) in a surface-dependent manner. Immunoassay analysis of cell culture keratinocyte-fibroblast paracrine relationship as characterized by IL-1β and KGF could not be established in the presence of IPNs, 0.1% glutaraldehyde-fixed gelatin hydrogels, and polycarbonate membranes. © 2004 Elsevier Ltd. All rights reserved.-
dc.languageeng-
dc.relation.ispartofBiomaterials-
dc.subjectPEG-
dc.subjectHydrogels-
dc.subjecthuKGF-
dc.subjectFibroblast co-culture-
dc.subjectCell adhesion-
dc.titleKeratinocyte-fibroblast paracrine interaction: The effects of substrate and culture condition-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.biomaterials.2004.09.054-
dc.identifier.pmid15621258-
dc.identifier.scopuseid_2-s2.0-11144250764-
dc.identifier.volume26-
dc.identifier.issue17-
dc.identifier.spage3673-
dc.identifier.epage3682-

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