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Article: Fibronectin modulates macrophage adhesion and FBGC formation: The role of RGD, PHSRN, and PRRARV domains

TitleFibronectin modulates macrophage adhesion and FBGC formation: The role of RGD, PHSRN, and PRRARV domains
Authors
KeywordsExtracellular matrix proteins
Polyethylene glycols
Leukocytes
Integrins
Inflammation
Issue Date2001
Citation
Journal of Biomedical Materials Research, 2001, v. 55, n. 1, p. 79-88 How to Cite?
AbstractTo probe the role of human plasma fibronectin in modulating human blood-derived macrophage adhesion and fusion to form multinucleated foreign-body giant cells (FBGC), a series of biomimetic oligopeptides based on the functional structure of fibronectin was designed and synthesized. Peptides incorporated the RGD and PHSRN integrin-binding sequences from FIII-10 and FIII-9 modules, respectively, and the PRRARV sequence from the C-terminal heparin-binding domain, either alone or in combination. Peptides were immobilized onto a polyethyleneglycol-based polymer substrate. The following conclusions were reached. Fibronectin modulated macrophage adhesion and the extent (i.e., size) of FBGC formation on control surfaces in the presence of serum proteins. Macrophages adhered to all substrates with relatively subtle differences between adhesion mediated by RGD, PHSRN, PRRARV, or combinations thereof. β1-integrin subunit was essential in macrophage adhesion to peptide-grafted networks in a receptor-peptide specific manner, whereas β3-integrin subunit was less important. Macrophage adhesion to PRRARV was mediated primarily by the direct interaction with integrins. RGD or PHSRN alone did not provide an adequate substrate for macrophage fusion to form FBGCs. However, the PHSRN synergistic site and the RGD site in a single oligopeptide provided a substrate for FBGC formation that was statistically comparable to that on the positive control material in the presence of serum proteins. This response was highly dependent upon the relative orientation between RGD and PHSRN. PRRARV did not support FBGC formation. These results demonstrate the importance of fibronectin and, specifically, the synergy between RGD and PHSRN domains, in supporting macrophage fusion to form FBGCs. © 2001 John Wiley & Sons, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/216160
ISSN
2004 Impact Factor: 3.652
2006 SCImago Journal Rankings: 0.474

 

DC FieldValueLanguage
dc.contributor.authorKao, Weiyuan John-
dc.contributor.authorLee, Damian-
dc.contributor.authorSchense, Jason C.-
dc.contributor.authorHubbell, Jeffrey A.-
dc.date.accessioned2015-08-25T10:22:05Z-
dc.date.available2015-08-25T10:22:05Z-
dc.date.issued2001-
dc.identifier.citationJournal of Biomedical Materials Research, 2001, v. 55, n. 1, p. 79-88-
dc.identifier.issn0021-9304-
dc.identifier.urihttp://hdl.handle.net/10722/216160-
dc.description.abstractTo probe the role of human plasma fibronectin in modulating human blood-derived macrophage adhesion and fusion to form multinucleated foreign-body giant cells (FBGC), a series of biomimetic oligopeptides based on the functional structure of fibronectin was designed and synthesized. Peptides incorporated the RGD and PHSRN integrin-binding sequences from FIII-10 and FIII-9 modules, respectively, and the PRRARV sequence from the C-terminal heparin-binding domain, either alone or in combination. Peptides were immobilized onto a polyethyleneglycol-based polymer substrate. The following conclusions were reached. Fibronectin modulated macrophage adhesion and the extent (i.e., size) of FBGC formation on control surfaces in the presence of serum proteins. Macrophages adhered to all substrates with relatively subtle differences between adhesion mediated by RGD, PHSRN, PRRARV, or combinations thereof. β1-integrin subunit was essential in macrophage adhesion to peptide-grafted networks in a receptor-peptide specific manner, whereas β3-integrin subunit was less important. Macrophage adhesion to PRRARV was mediated primarily by the direct interaction with integrins. RGD or PHSRN alone did not provide an adequate substrate for macrophage fusion to form FBGCs. However, the PHSRN synergistic site and the RGD site in a single oligopeptide provided a substrate for FBGC formation that was statistically comparable to that on the positive control material in the presence of serum proteins. This response was highly dependent upon the relative orientation between RGD and PHSRN. PRRARV did not support FBGC formation. These results demonstrate the importance of fibronectin and, specifically, the synergy between RGD and PHSRN domains, in supporting macrophage fusion to form FBGCs. © 2001 John Wiley & Sons, Inc.-
dc.languageeng-
dc.relation.ispartofJournal of Biomedical Materials Research-
dc.subjectExtracellular matrix proteins-
dc.subjectPolyethylene glycols-
dc.subjectLeukocytes-
dc.subjectIntegrins-
dc.subjectInflammation-
dc.titleFibronectin modulates macrophage adhesion and FBGC formation: The role of RGD, PHSRN, and PRRARV domains-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1002/1097-4636(200104)55:1<79::AID-JBM110>3.0.CO;2-Z-
dc.identifier.pmid11426401-
dc.identifier.scopuseid_2-s2.0-0035135285-
dc.identifier.volume55-
dc.identifier.issue1-
dc.identifier.spage79-
dc.identifier.epage88-

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