File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Role for interleukin-4 in foreign-body giant cell formation on a poly(etherurethane urea) in vivo

TitleRole for interleukin-4 in foreign-body giant cell formation on a poly(etherurethane urea) in vivo
Authors
Issue Date1995
Citation
Journal of Biomedical Materials Research, 1995, v. 29, n. 10, p. 1267-1275 How to Cite?
AbstractInterleukin-4 (IL-4) was previously shown to induce extensive macrophage fusion to form foreign-body giant cells (FBGCs) in vitro. In the present study, our goal was to extend these findings to an in vivo test environment on biomaterials. The subcutaneous cage-implant system was modified for mice to elucidate IL-4 participation in mediating FBGC formation in vivo. Exudate leukocyte concentrations from cages containing poly(etherurethane urea) (PEUU A') and empty cage controls indicated a similar inflammatory response that turned toward resolution by 14 days postimplantation, thus confirming the applicability of the cage-implant system in mice. FBGC kinetic analysis showed that the formation of mouse FBGCs occurs through the fusion of adherent macrophages at a constant rate up to 14 days of implantation. Purified goat anti-mouse IL-4 neutralizing antibody (IL4Ab) or normal goat nonspecific control IgG (gtlgG) at various concentrations, or recombinant murine IL-4 (muIL4) was injected into the implanted cages containing PEUU A' every 2 days for 7 days. The injection of IL4Ab significantly decreased the FBGC density on PEUU A' cage-implanted in mice, when compared with the nonspecific IgG or PBS injection controls. Conversely, the FBGC density was significantly increased by the injection of muIL4 when compared with nonspecific IgG and PBS injection controls. Adherent macrophage density, FBGC morphology, FBGC average size, and size distribution were not significantly different among IL4Ab, nonspecific control gtlgG, muIL4, and PBS control groups. Our data suggest that IL-4 participates in FBGC formation on biomaterials in vivo. | Interleukin-4 (IL-4) has been shown previously to induce extensive macrophage fusion to form foreign-body giant cells (FBGCs) in vitro. In this study, previous findings are extended to an in vivo test environment on biomaterials. The subcutaneous cage-implant system is modified for mice to elucidate IL-4 participation in mediating FBGC formation in vivo. FBGC kinetic analysis shows that the formation of mouse FBGC occurs through the fusion of adherent macrophages at a constant rate up to 14 days of implantation. Purified goat anti-mouse IL-4 neutralizing antibody or normal goat nonspecific control IgG at various concentrations, or recombinant murine IL-4 is injected into the implanted cages containing poly(etherurethane urea) every 2 days for 7 days. The data suggest that IL-4 participates in FBGC formation on biomaterials in vivo.
Persistent Identifierhttp://hdl.handle.net/10722/216145
ISSN
2004 Impact Factor: 3.652
2006 SCImago Journal Rankings: 0.474

 

DC FieldValueLanguage
dc.contributor.authorKao, W. J.-
dc.contributor.authorMcNally, A. K.-
dc.contributor.authorHiltner, A.-
dc.contributor.authorAnderson, J. M.-
dc.date.accessioned2015-08-25T10:21:46Z-
dc.date.available2015-08-25T10:21:46Z-
dc.date.issued1995-
dc.identifier.citationJournal of Biomedical Materials Research, 1995, v. 29, n. 10, p. 1267-1275-
dc.identifier.issn0021-9304-
dc.identifier.urihttp://hdl.handle.net/10722/216145-
dc.description.abstractInterleukin-4 (IL-4) was previously shown to induce extensive macrophage fusion to form foreign-body giant cells (FBGCs) in vitro. In the present study, our goal was to extend these findings to an in vivo test environment on biomaterials. The subcutaneous cage-implant system was modified for mice to elucidate IL-4 participation in mediating FBGC formation in vivo. Exudate leukocyte concentrations from cages containing poly(etherurethane urea) (PEUU A') and empty cage controls indicated a similar inflammatory response that turned toward resolution by 14 days postimplantation, thus confirming the applicability of the cage-implant system in mice. FBGC kinetic analysis showed that the formation of mouse FBGCs occurs through the fusion of adherent macrophages at a constant rate up to 14 days of implantation. Purified goat anti-mouse IL-4 neutralizing antibody (IL4Ab) or normal goat nonspecific control IgG (gtlgG) at various concentrations, or recombinant murine IL-4 (muIL4) was injected into the implanted cages containing PEUU A' every 2 days for 7 days. The injection of IL4Ab significantly decreased the FBGC density on PEUU A' cage-implanted in mice, when compared with the nonspecific IgG or PBS injection controls. Conversely, the FBGC density was significantly increased by the injection of muIL4 when compared with nonspecific IgG and PBS injection controls. Adherent macrophage density, FBGC morphology, FBGC average size, and size distribution were not significantly different among IL4Ab, nonspecific control gtlgG, muIL4, and PBS control groups. Our data suggest that IL-4 participates in FBGC formation on biomaterials in vivo. | Interleukin-4 (IL-4) has been shown previously to induce extensive macrophage fusion to form foreign-body giant cells (FBGCs) in vitro. In this study, previous findings are extended to an in vivo test environment on biomaterials. The subcutaneous cage-implant system is modified for mice to elucidate IL-4 participation in mediating FBGC formation in vivo. FBGC kinetic analysis shows that the formation of mouse FBGC occurs through the fusion of adherent macrophages at a constant rate up to 14 days of implantation. Purified goat anti-mouse IL-4 neutralizing antibody or normal goat nonspecific control IgG at various concentrations, or recombinant murine IL-4 is injected into the implanted cages containing poly(etherurethane urea) every 2 days for 7 days. The data suggest that IL-4 participates in FBGC formation on biomaterials in vivo.-
dc.languageeng-
dc.relation.ispartofJournal of Biomedical Materials Research-
dc.titleRole for interleukin-4 in foreign-body giant cell formation on a poly(etherurethane urea) in vivo-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jbm.820291014-
dc.identifier.pmid8557729-
dc.identifier.scopuseid_2-s2.0-0029380449-
dc.identifier.volume29-
dc.identifier.issue10-
dc.identifier.spage1267-
dc.identifier.epage1275-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats