File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: A bioengineered heterotypic stroma-cancer microenvironment model to study pancreatic ductal adenocarcinoma

TitleA bioengineered heterotypic stroma-cancer microenvironment model to study pancreatic ductal adenocarcinoma
Authors
Issue Date2013
Citation
Lab on a Chip - Miniaturisation for Chemistry and Biology, 2013, v. 13, n. 19, p. 3965-3975 How to Cite?
AbstractInteractions between neoplastic epithelial cells and components of a reactive stroma in pancreatic ductal adenocarcinoma (PDAC) are of key significance behind the disease's dismal prognosis. Despite extensive published research in the importance of stroma-cancer interactions in other cancers and experimental evidence supporting the importance of the microenvironment in PDAC progression, a reproducible three-dimensional (3D) in vitro model for exploring stroma-cancer interplay and evaluating therapeutics in a physiologically relevant context has been lacking. We introduce a humanized microfluidic model of the PDAC microenvironment incorporating multicellularity, extracellular matrix (ECM) components, and a spatially defined 3D microarchitecture. Pancreatic stellate cells (PSCs) isolated from clinically-evaluated human tissue specimens were co-cultured with pancreatic ductal adenocarcinoma cells as an accessible 3D construct that maintained important tissue features and disease behavior. Multiphoton excitation (MPE) and Second Harmonic Generation (SHG) imaging techniques were utilized to image the intrinsic signal of stromal collagen in human pancreatic tissues and live cell-collagen interactions within the optically-accessible microfluidic tissue model. We further evaluated the dose-response of the model with the anticancer agent paclitaxel. This bioengineered model of the PDAC stroma-cancer microenvironment provides a complementary platform to elucidate the complex stroma-cancer interrelationship and to evaluate the efficacy of potential therapeutics in a humanized system that closely recapitulates key PDAC microenvironment characteristics. © 2013 The Royal Society of Chemistry.
Persistent Identifierhttp://hdl.handle.net/10722/216122
ISSN
2015 Impact Factor: 5.586
2015 SCImago Journal Rankings: 2.373

 

DC FieldValueLanguage
dc.contributor.authorDrifka, Cole R.-
dc.contributor.authorEliceiri, Kevin W.-
dc.contributor.authorWeber, Sharon M.-
dc.contributor.authorKao, W. John-
dc.date.accessioned2015-08-25T10:20:58Z-
dc.date.available2015-08-25T10:20:58Z-
dc.date.issued2013-
dc.identifier.citationLab on a Chip - Miniaturisation for Chemistry and Biology, 2013, v. 13, n. 19, p. 3965-3975-
dc.identifier.issn1473-0197-
dc.identifier.urihttp://hdl.handle.net/10722/216122-
dc.description.abstractInteractions between neoplastic epithelial cells and components of a reactive stroma in pancreatic ductal adenocarcinoma (PDAC) are of key significance behind the disease's dismal prognosis. Despite extensive published research in the importance of stroma-cancer interactions in other cancers and experimental evidence supporting the importance of the microenvironment in PDAC progression, a reproducible three-dimensional (3D) in vitro model for exploring stroma-cancer interplay and evaluating therapeutics in a physiologically relevant context has been lacking. We introduce a humanized microfluidic model of the PDAC microenvironment incorporating multicellularity, extracellular matrix (ECM) components, and a spatially defined 3D microarchitecture. Pancreatic stellate cells (PSCs) isolated from clinically-evaluated human tissue specimens were co-cultured with pancreatic ductal adenocarcinoma cells as an accessible 3D construct that maintained important tissue features and disease behavior. Multiphoton excitation (MPE) and Second Harmonic Generation (SHG) imaging techniques were utilized to image the intrinsic signal of stromal collagen in human pancreatic tissues and live cell-collagen interactions within the optically-accessible microfluidic tissue model. We further evaluated the dose-response of the model with the anticancer agent paclitaxel. This bioengineered model of the PDAC stroma-cancer microenvironment provides a complementary platform to elucidate the complex stroma-cancer interrelationship and to evaluate the efficacy of potential therapeutics in a humanized system that closely recapitulates key PDAC microenvironment characteristics. © 2013 The Royal Society of Chemistry.-
dc.languageeng-
dc.relation.ispartofLab on a Chip - Miniaturisation for Chemistry and Biology-
dc.titleA bioengineered heterotypic stroma-cancer microenvironment model to study pancreatic ductal adenocarcinoma-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1039/c3lc50487e-
dc.identifier.pmid23959166-
dc.identifier.scopuseid_2-s2.0-84883267761-
dc.identifier.volume13-
dc.identifier.issue19-
dc.identifier.spage3965-
dc.identifier.epage3975-
dc.identifier.eissn1473-0189-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats