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Article: Selective interaction of Hpn-like protein with nickel, zinc and bismuth in vitro and in cells by FRET
Title | Selective interaction of Hpn-like protein with nickel, zinc and bismuth in vitro and in cells by FRET |
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Authors | |
Keywords | FRET Metal selectivity Metallodrugs Metalloproteins Nickel storage |
Issue Date | 2015 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/jinorgbio |
Citation | Journal of Inorganic Biochemistry, 2015, v. 142, p. 8-14 How to Cite? |
Abstract | Hpn-like (Hpnl) is a unique histidine- and glutamine-rich protein found only in Helicobacter pylori and plays a role on nickel homeostasis.Weconstructed the fluorescent sensor proteins CYHpnl and CYHpnl_1-48 (C-terminal glutamine-rich region truncated) using enhanced cyan and yellow fluorescent proteins (eCFP and eYFP) as the donor–acceptor pair to monitor the interactions of Hpnl with metal ions and to elucidate the role of conserved Glu-rich sequence in Hpnl by fluorescence resonance energy transfer (FRET). CYHpnl and CYHpnl_1-48 exhibited largest responses towards Ni(II) and Zn(II) over other metals studied and the binding of Bi(III) to CYHpnl was observed in the presence of an excess amount of Bi(III) ions (Kd =115±4.8 μM). Moreover, both CYHpnl and CYHpnl_1-48 showed positive FRET responses towards the binding to Ni(II) and Zn(II) in Escherichia coli cells overexpressing CYHpnl and CYHpnl_1-48, whereas a decrease in FRET upon Bi(III)-binding in E. coli cells overexpressing the latter. Our study provides clear evidence on Hpnl binding to nickel in cells, and intracellular interaction of Hpnl with Bi(III) could disrupt the protein function, thus probably contributing to the efficacy of Bi(III) drugs against H. pylori. |
Persistent Identifier | http://hdl.handle.net/10722/215150 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.614 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chang, YY | - |
dc.contributor.author | Lai, YT | - |
dc.contributor.author | Cheng, T | - |
dc.contributor.author | Wang, H | - |
dc.contributor.author | Yang, Y | - |
dc.contributor.author | Sun, H | - |
dc.date.accessioned | 2015-08-21T13:16:09Z | - |
dc.date.available | 2015-08-21T13:16:09Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Journal of Inorganic Biochemistry, 2015, v. 142, p. 8-14 | - |
dc.identifier.issn | 0162-0134 | - |
dc.identifier.uri | http://hdl.handle.net/10722/215150 | - |
dc.description.abstract | Hpn-like (Hpnl) is a unique histidine- and glutamine-rich protein found only in Helicobacter pylori and plays a role on nickel homeostasis.Weconstructed the fluorescent sensor proteins CYHpnl and CYHpnl_1-48 (C-terminal glutamine-rich region truncated) using enhanced cyan and yellow fluorescent proteins (eCFP and eYFP) as the donor–acceptor pair to monitor the interactions of Hpnl with metal ions and to elucidate the role of conserved Glu-rich sequence in Hpnl by fluorescence resonance energy transfer (FRET). CYHpnl and CYHpnl_1-48 exhibited largest responses towards Ni(II) and Zn(II) over other metals studied and the binding of Bi(III) to CYHpnl was observed in the presence of an excess amount of Bi(III) ions (Kd =115±4.8 μM). Moreover, both CYHpnl and CYHpnl_1-48 showed positive FRET responses towards the binding to Ni(II) and Zn(II) in Escherichia coli cells overexpressing CYHpnl and CYHpnl_1-48, whereas a decrease in FRET upon Bi(III)-binding in E. coli cells overexpressing the latter. Our study provides clear evidence on Hpnl binding to nickel in cells, and intracellular interaction of Hpnl with Bi(III) could disrupt the protein function, thus probably contributing to the efficacy of Bi(III) drugs against <i>H. pylori</i>. | - |
dc.language | eng | - |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/jinorgbio | - |
dc.relation.ispartof | Journal of Inorganic Biochemistry | - |
dc.rights | © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | FRET | - |
dc.subject | Metal selectivity | - |
dc.subject | Metallodrugs | - |
dc.subject | Metalloproteins | - |
dc.subject | Nickel storage | - |
dc.title | Selective interaction of Hpn-like protein with nickel, zinc and bismuth in vitro and in cells by FRET | - |
dc.type | Article | - |
dc.identifier.email | Sun, H: hsun@hku.hk | - |
dc.identifier.authority | Sun, H=rp00777 | - |
dc.description.nature | postprint | - |
dc.identifier.doi | 10.1016/j.jinorgbio.2014.09.010 | - |
dc.identifier.scopus | eid_2-s2.0-84908576822 | - |
dc.identifier.hkuros | 247650 | - |
dc.identifier.volume | 142 | - |
dc.identifier.spage | 8 | - |
dc.identifier.epage | 14 | - |
dc.identifier.isi | WOS:000346880400002 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0162-0134 | - |