File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Conversion of non-adipogenic fibroblasts into adipocytes by a defined hormone mixture

TitleConversion of non-adipogenic fibroblasts into adipocytes by a defined hormone mixture
Authors
Issue Date2015
Citation
Biochemical Journal, 2015, v. 467 n. 3, p. 487-494 How to Cite?
AbstractObesity is accompanied by an increase in the size and the number of adipocytes. As adipocytes are thought to differentiate from pre-adipocytes, we postulate that non-adipogenic fibroblasts contribute to adipocyte formation under certain conditions such as obesity. We report for the first time that NIH-3T3 fibroblasts, which are generally considered to be non-adipogenic, can be converted into mature adipocytes by treatment with a defined hormone mixture comprising EGF (epidermal growth factor), HGF (hepatocyte growth factor), Dex (dexamethasone) and insulin. Furthermore, NIH-3T3 cells transplanted into obese immunodeficient NOD/SCID (non-obese diabetic/severe combined immunodeficient) mice formed adipocytes in vivo. Interestingly, the mixture elicited conversion of NIH-3T3 cells directly into adipocytes without a preceding pre-adipocyte stage. Functional analysis revealed that each component of the mixture was necessary for the induction of adipogenesis, including Dex which inhibited the cell proliferation stimulated by EGF. Upon profiling the signalling pathways employed by EGF and HGF, we found STAT5 (signal transducer and activator of transcription 5) signalling to be activated, predominantly at the levels of both transcription and post-translational modification. Inhibition of the STAT5 pathway, either by genetic knockdown or a specific pharmacological agent, blocked adipogenesis in NIH-3T3 cells. Taken together, these data not only establish a newly recognized grouping of factors that can induce trans-differentiation of non-adipogenic fibroblasts into adipocytes, but also give us a greater understanding of obesity.
Persistent Identifierhttp://hdl.handle.net/10722/214325

 

DC FieldValueLanguage
dc.contributor.authorNie, T-
dc.contributor.authorHui, X-
dc.contributor.authorGao, X-
dc.contributor.authorNie, B-
dc.contributor.authorMao, L-
dc.contributor.authorTang, X-
dc.contributor.authorYuan, R-
dc.contributor.authorLi, K-
dc.contributor.authorLi, P-
dc.contributor.authorXu, A-
dc.date.accessioned2015-08-21T11:14:47Z-
dc.date.available2015-08-21T11:14:47Z-
dc.date.issued2015-
dc.identifier.citationBiochemical Journal, 2015, v. 467 n. 3, p. 487-494-
dc.identifier.urihttp://hdl.handle.net/10722/214325-
dc.description.abstractObesity is accompanied by an increase in the size and the number of adipocytes. As adipocytes are thought to differentiate from pre-adipocytes, we postulate that non-adipogenic fibroblasts contribute to adipocyte formation under certain conditions such as obesity. We report for the first time that NIH-3T3 fibroblasts, which are generally considered to be non-adipogenic, can be converted into mature adipocytes by treatment with a defined hormone mixture comprising EGF (epidermal growth factor), HGF (hepatocyte growth factor), Dex (dexamethasone) and insulin. Furthermore, NIH-3T3 cells transplanted into obese immunodeficient NOD/SCID (non-obese diabetic/severe combined immunodeficient) mice formed adipocytes in vivo. Interestingly, the mixture elicited conversion of NIH-3T3 cells directly into adipocytes without a preceding pre-adipocyte stage. Functional analysis revealed that each component of the mixture was necessary for the induction of adipogenesis, including Dex which inhibited the cell proliferation stimulated by EGF. Upon profiling the signalling pathways employed by EGF and HGF, we found STAT5 (signal transducer and activator of transcription 5) signalling to be activated, predominantly at the levels of both transcription and post-translational modification. Inhibition of the STAT5 pathway, either by genetic knockdown or a specific pharmacological agent, blocked adipogenesis in NIH-3T3 cells. Taken together, these data not only establish a newly recognized grouping of factors that can induce trans-differentiation of non-adipogenic fibroblasts into adipocytes, but also give us a greater understanding of obesity.-
dc.languageeng-
dc.relation.ispartofBiochemical Journal-
dc.titleConversion of non-adipogenic fibroblasts into adipocytes by a defined hormone mixture-
dc.typeArticle-
dc.identifier.emailHui, X: hannahui@hkucc.hku.hk-
dc.identifier.emailXu, A: amxu@hkucc.hku.hk-
dc.identifier.authorityXu, A=rp00485-
dc.identifier.doi10.1042/BJ20140727-
dc.identifier.hkuros246869-
dc.identifier.volume467-
dc.identifier.issue3-
dc.identifier.spage487-
dc.identifier.epage494-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats